Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F17 I0 R2
|
159 |
90.8 |
4763082 |
92.7% |
4415377 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,824,288 |
G→A |
8.1% |
D452D (GAC→GAT) |
hypF ← |
carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,824,288 | 0 | G | A | 8.1%
| 234.1
/ 8.7
| 74 | D452D (GAC→GAT) | hypF | carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases |
| Reads supporting (aligned to +/- strand): ref base G (29/39); new base A (3/3); total (32/42) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01 |
ATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGC > minE/1824219‑1824349
|
atgCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGt < 1:845121/71‑1 (MQ=255)
gCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCa > 1:1109051/1‑71 (MQ=255)
gCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCa > 1:720887/1‑71 (MQ=255)
cAGTACCGTTTTCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCa < 1:4606304/70‑1 (MQ=255)
aGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATg > 1:2077876/1‑71 (MQ=255)
aGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATg > 1:162727/1‑71 (MQ=255)
aCCGTTTGCGTAGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCATCATGca > 1:2617037/1‑70 (MQ=255)
ccGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGcaga < 1:1331171/71‑3 (MQ=255)
ccGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGcaca < 1:3210985/71‑1 (MQ=255)
ccGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGcaca < 1:2415235/71‑1 (MQ=255)
ccGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGcac < 1:1376538/70‑1 (MQ=255)
ccGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGcac < 1:230331/70‑1 (MQ=255)
ccGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGcac < 1:1158461/70‑1 (MQ=255)
ccGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGca > 1:1153447/1‑69 (MQ=255)
ccGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGca > 1:4301469/1‑69 (MQ=255)
tGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGt < 1:2299363/70‑1 (MQ=255)
tGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGt < 1:4631037/70‑1 (MQ=255)
cGTCGGCAGATTCATTTCACGCGCCCACTGGCTTGAGACATAGCCCGGATGCGCGTCATGCACAACGTAt < 1:1767945/70‑1 (MQ=255)
cGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTAtt > 1:632069/1‑71 (MQ=255)
cGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCAGGATGCGCGTCATGCACAACGTAtt < 1:198718/71‑1 (MQ=255)
tCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGc < 1:4398569/71‑1 (MQ=255)
tCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGc < 1:4244354/71‑1 (MQ=255)
tCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGc < 1:3046037/71‑1 (MQ=255)
ggCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCgg > 1:3083874/1‑71 (MQ=255)
ggCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCg < 1:1039020/70‑1 (MQ=255)
aTTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTa > 1:1450710/1‑70 (MQ=255)
tCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCg < 1:2928106/63‑1 (MQ=255)
tCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCg < 1:3287459/63‑1 (MQ=255)
tCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCg < 1:3219127/63‑1 (MQ=255)
tCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCg < 1:4409102/63‑1 (MQ=255)
tCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTaaaa > 1:3745033/1‑71 (MQ=255)
tCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTaa < 1:2288248/69‑1 (MQ=255)
tCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTaa < 1:3126627/69‑1 (MQ=255)
cATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGt < 1:2465780/56‑1 (MQ=255)
cATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGt < 1:4502781/56‑1 (MQ=255)
tCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCg > 1:4366508/1‑69 (MQ=255)
cACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCg > 1:113487/1‑68 (MQ=255)
cgcgCCCACTGGCTGGAGACATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGAt > 1:2231729/1‑71 (MQ=255)
gcgcCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATg < 1:4426735/71‑1 (MQ=255)
gcgcCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATg < 1:3321476/71‑1 (MQ=255)
cgcCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGt < 1:4258615/71‑1 (MQ=255)
cccACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTaaaa > 1:3580727/1‑59 (MQ=255)
ccACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGtttt > 1:4468023/1‑71 (MQ=255)
aCTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTg > 1:1496647/1‑70 (MQ=255)
aCTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTg > 1:4745326/1‑70 (MQ=255)
gtggctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATg < 1:893082/63‑1 (MQ=255)
ggctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCAt > 1:3746463/1‑70 (MQ=255)
gctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCAt < 1:4662884/69‑1 (MQ=255)
gctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCAt > 1:3049647/1‑69 (MQ=255)
gctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCAt < 1:2591730/69‑1 (MQ=255)
gctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATc < 1:1175588/70‑1 (MQ=255)
gctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATc < 1:2062473/70‑1 (MQ=255)
gctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATc < 1:397388/70‑1 (MQ=255)
gctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATc < 1:2118032/70‑1 (MQ=255)
gagaCATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCg > 1:3802699/1‑71 (MQ=255)
gagaCATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCg > 1:3983930/1‑71 (MQ=255)
aCATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTaa > 1:3111742/1‑71 (MQ=255)
aCATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTTGATGttt < 1:3424063/57‑1 (MQ=255)
aCATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGttt < 1:2575433/57‑1 (MQ=255)
aCATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGttt < 1:3171997/57‑1 (MQ=255)
cATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGCTGtt > 1:1491502/1‑55 (MQ=255)
cATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGt < 1:2495351/54‑1 (MQ=255)
tAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGttt > 1:2758085/1‑54 (MQ=255)
tAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGttt > 1:2952266/1‑54 (MQ=255)
tAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGttt > 1:571452/1‑54 (MQ=255)
tAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGc < 1:2082073/71‑1 (MQ=255)
gCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAg > 1:1174192/1‑46 (MQ=255)
gCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGa > 1:4271988/1‑47 (MQ=255)
gCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGa > 1:2965329/1‑47 (MQ=255)
cGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTcgc < 1:4310852/71‑1 (MQ=255)
cGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTcgc < 1:2734229/71‑1 (MQ=255)
ggATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGAt > 1:108199/1‑44 (MQ=255)
ggATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGAt > 1:1213881/1‑44 (MQ=255)
tGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGt < 1:2852777/43‑1 (MQ=255)
cgcgTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGt > 1:2046531/1‑55 (MQ=255)
cgcgTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGt > 1:867957/1‑55 (MQ=255)
gTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGc < 1:839674/57‑1 (MQ=255)
|
ATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGC > minE/1824219‑1824349
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
