Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F17 I0 R2	159	90.8	4763082	92.7%	4415377	60.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,489,124	G→A	5.2%	T41T (ACG→ACA)	dnaN →	DNA polymerase III, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,489,124	0	G	A	5.2%	456.5 / 9.6	134	T41T (ACG→ACA)	dnaN	DNA polymerase III, beta subunit
Reads supporting (aligned to +/- strand): ref base G (62/65); new base A (3/4); total (65/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 135 total aligned reads displayed.

CGGTCCGTTAGGTGGTCGTCCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTGCGCTGGTTCAGCCACACGA  >  minE/2489055‑2489192

cGGTCCGTTAGGTGGTCGTCCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGt                                                                     <  1:4355740/71‑1 (MQ=255)

   tCCGTTAGGTGGTCGTCCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACg                                                                      <  1:4171994/67‑1 (MQ=255)

     cGTTAGGTGGTCGTCCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTc                                                                 <  1:2215348/70‑1 (MQ=255)

     cGTTAGGTGGTCGTCCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTc                                                                 >  1:1297371/1‑70 (MQ=255)

       ttAGGTGGTCGTCCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCt                                                              <  1:965601/71‑1 (MQ=255)

       ttAGGTGGTCGTCCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCt                                                              >  1:1709463/1‑71 (MQ=255)

         aGGTGGTCGTCCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTg                                                             <  1:1662524/70‑1 (MQ=255)

         aGGTGGTCGTCCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTg                                                             <  1:248281/70‑1 (MQ=255)

         aGGTGGTCGTCCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTg                                                             <  1:2965230/70‑1 (MQ=255)

         aGGTGGTCGTCCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTg                                                             <  1:674860/70‑1 (MQ=255)

              gtcgtcCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCgg                                                        >  1:3891875/1‑70 (MQ=255)

              gtcgtcCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCgg                                                        <  1:3114725/70‑1 (MQ=255)

              gtcgtcCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCgg                                                        >  1:4442342/1‑70 (MQ=255)

              ggcgtcCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCgg                                                        <  1:458633/68‑1 (MQ=255)

               tcgtcCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGtt                                                                    <  1:2743387/57‑1 (MQ=255)

               tcgtcCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGtt                                                                    <  1:493330/57‑1 (MQ=255)

               tcgtcCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGtt                                                                    <  1:1057618/57‑1 (MQ=255)

               tcgtcCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGtt                                                                    <  1:1369516/57‑1 (MQ=255)

               tcgtcCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTa                                                      <  1:3569362/71‑1 (MQ=255)

                cgtcCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTa                                                      >  1:249559/1‑70 (MQ=255)

                 gtcCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGt                                                                  >  1:3381253/1‑57 (MQ=255)

                 gtcCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGt                                                                  >  1:2816530/1‑57 (MQ=255)

                    cTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGa                                                  >  1:1441239/1‑70 (MQ=255)

                    cTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGAt                                                 >  1:3159919/1‑71 (MQ=255)

                       cGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTa                                                      >  1:1594964/1‑63 (MQ=255)

                       cGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTa                                                      >  1:3830943/1‑63 (MQ=255)

                       cGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTa                                                      >  1:2416651/1‑63 (MQ=255)

                        gCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGAc                                                           <  1:456731/57‑1 (MQ=255)

                          tACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCg                                                         >  1:4675872/1‑57 (MQ=255)

                          tACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCg                                                         >  1:3086941/1‑57 (MQ=255)

                          tACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCg                                                         >  1:1519938/1‑57 (MQ=255)

                          tACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCg                                                         >  1:477109/1‑57 (MQ=255)

                          tACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACATTGTCGCTGACCGGTACTg                                                   <  1:897893/63‑1 (MQ=255)

                          tACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACATTGTCGCTGACCGGTACTg                                                   <  1:4232329/63‑1 (MQ=255)

                            ccGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCgaga                                          <  1:3085716/70‑1 (MQ=255)

                            ccGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCgag                                           <  1:1722721/69‑1 (MQ=255)

                            ccGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACATTGTCGCTGACCGGTACTGAt                                                 >  1:733206/1‑63 (MQ=255)

                            ccGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACATTGTCGCTGACCGGTACTGAt                                                 >  1:2197051/1‑63 (MQ=255)

                             cGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTg                                                             <  1:2273271/50‑1 (MQ=255)

                                ttCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGAtc                                                <  1:2697632/60‑1 (MQ=255)

                                 tctcGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCg                                                         <  1:4175446/50‑1 (MQ=255)

                                 tctcGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGaaa                                    >  1:4211143/1‑71 (MQ=255)

                                 tctcGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGaaa                                    >  1:2975426/1‑71 (MQ=255)

                                   tcGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCg                                             <  1:1189971/60‑1 (MQ=255)

                                   tcGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCg                                             <  1:4703307/60‑1 (MQ=255)

                                   tcGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATgg                                       >  1:837692/1‑66 (MQ=255)

                                   tcGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGaaa                                    >  1:1090294/1‑69 (MQ=255)

                                     ggTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAAtggt                                <  1:1187497/71‑1 (MQ=255)

                                     ggTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAAtggt                                <  1:2062100/71‑1 (MQ=255)

                                     ggTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAAtg                                  <  1:3729841/69‑1 (MQ=255)

                                      gTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCg                                                                <  1:4316105/38‑1 (MQ=255)

                                      gTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCg                                                                <  1:3688147/38‑1 (MQ=255)

                                        aaTCTGCTGTTACAGGTTGCTGACGGTATGTTGTCGCTGACCGGTa                                                      >  1:1729116/1‑46 (MQ=255)

                                        aaTCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAAtggtgg                              >  1:803677/1‑70 (MQ=255)

                                        aaTCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAAtggt                                <  1:109844/68‑1 (MQ=255)

                                         aTCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCa                            >  1:4394251/1‑71 (MQ=255)

                                         aTCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCa                            >  1:4291963/1‑71 (MQ=255)

                                          tCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCgg                                                        <  1:200478/42‑1 (MQ=255)

                                          tCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCgg                                                        <  1:2654329/42‑1 (MQ=255)

                                           ctgctgTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACg                          >  1:1179379/1‑71 (MQ=255)

                                           ctgctgTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACg                          >  1:4230264/1‑71 (MQ=255)

                                            tgctgTTACAGGTTGCTGACGGTACGTTGTCGCTGa                                                            >  1:4045093/1‑36 (MQ=255)

                                            tgctgTTACAGGTTGCTGACGGTACGTTGTCGCTGa                                                            >  1:2528845/1‑36 (MQ=255)

                                              ctgTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACgtgt                       <  1:3421824/71‑1 (MQ=255)

                                              ctgTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACgt                         <  1:954698/69‑1 (MQ=255)

                                              ctgTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACgt                         <  1:4055294/69‑1 (MQ=255)

                                               tgTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGtt                      >  1:4245553/1‑71 (MQ=255)

                                                 ttACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCgag                                           <  1:1070585/48‑1 (MQ=255)

                                                 ttACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTgc                    <  1:666985/71‑1 (MQ=255)

                                                 ttACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTgc                    <  1:2470861/71‑1 (MQ=255)

                                                 ttACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTg                     <  1:525490/70‑1 (MQ=255)

                                                   aCAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGaaa                                    >  1:1909858/1‑53 (MQ=255)

                                                   aCAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGaaa                                    >  1:3045047/1‑53 (MQ=255)

                                                   aCAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTgcgc                  >  1:190361/1‑71 (MQ=255)

                                                     aGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTGCGCTg                >  1:136281/1‑71 (MQ=255)

                                                        ttGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTGCGCTGGt              <  1:4446931/70‑1 (MQ=255)

                                                           cTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTGCGCTGGTTCa           <  1:4566780/70‑1 (MQ=255)

                                                            tGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGaaa                                    >  1:512780/1‑44 (MQ=255)

                                                            tGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTGCGCTGGTTCAGc         >  1:3578178/1‑71 (MQ=255)

                                                            tGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTGCGCTGGTTCAGc         >  1:2110156/1‑71 (MQ=255)

                                                             gACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGGAATGGTGGCACGTGTTGCGCTGGTTCAGc         >  1:2910985/1‑70 (MQ=255)

                                                              aCGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACg                          >  1:4451154/1‑52 (MQ=255)

                                                              aCGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTGCGCTGGTTCAGc         <  1:1025406/69‑1 (MQ=255)

                                                              aCGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTGCGCTGGTTCAGCca       >  1:1543750/1‑71 (MQ=255)

                                                              aCGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTGCGCTGGTTCAGCca       <  1:4268525/71‑1 (MQ=255)

                                                              aCGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTGCGCTGGTTCAGCca       >  1:4053822/1‑71 (MQ=255)

                                                              aCGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTGCGCTGGTTCAGCca       >  1:2077484/1‑71 (MQ=255)

                                                              aCGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTGCGCTGGTTCAGCca       >  1:1467511/1‑71 (MQ=255)

                                                              aCGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTGCGATGGTTCAGCca       >  1:1902153/1‑71 (MQ=255)

                                                                ggTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTGCGCTGGTTCAGCcaca     >  1:1650877/1‑71 (MQ=255)

                                                                 gTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGaaa                                    <  1:4582771/39‑1 (MQ=255)

                                                                 gTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTGCGCTGGTTCAGCCacac    <  1:702563/71‑1 (MQ=255)

                                                                 gTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTGCGCTGGTTCAGCCacac    <  1:1575264/71‑1 (MQ=255)

                                                                 gTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTGCGCTGGTTCAGCCacac    <  1:4610076/71‑1 (MQ=255)

                                                                  tACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTGCGCTGGtt             <  1:4405365/61‑1 (MQ=255)

                                                                  tACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTGCGCTGGtt             <  1:1110505/61‑1 (MQ=255)

                                                                  tACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTGCGCTGGTTCAGCCacac    <  1:2633089/70‑1 (MQ=255)

                                                                   aCGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGATGCGCTGGTTCAGCCacac    >  1:944602/1‑69 (MQ=255)

                                                                   aCGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGATGCGCTGGTTCAGCCacac    >  1:950063/1‑69 (MQ=255)

                                                                    cGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAAtg                                  >  1:3770198/1‑38 (MQ=255)

CGGTCCGTTAGGTGGTCGTCCTACGCTACCGATTCTCGGTAATCTGCTGTTACAGGTTGCTGACGGTACGTTGTCGCTGACCGGTACTGATCTCGAGATGGAAATGGTGGCACGTGTTGCGCTGGTTCAGCCACACGA  >  minE/2489055‑2489192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A