Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F17 I0 R2	159	90.8	4763082	92.7%	4415377	60.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,605,853	T→C	7.1%	V14V (GTT→GTC)	yhiO →	predicted universal stress (ethanol tolerance) protein B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,605,853	0	T	C	7.1%	330.8 / 12.8	112	V14V (GTT→GTC)	yhiO	predicted universal stress (ethanol tolerance) protein B
Reads supporting (aligned to +/- strand): ref base T (49/55); new base C (2/6); total (51/61)
Fisher's exact test for biased strand distribution p-value = 2.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 117 total aligned reads displayed.

CGCGGGCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATC  >  minE/2605786‑2605923

cgcgGGCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGttt                                                                       <  1:3695629/69‑1 (MQ=255)

cgcgGGCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGc                                                                     <  1:2699819/71‑1 (MQ=255)

cgcgGGCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTg                                                                      <  1:1399040/70‑1 (MQ=255)

 gcgGGCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCa                                                                    >  1:2226124/1‑71 (MQ=255)

 gcgGGCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCa                                                                    >  1:1496111/1‑71 (MQ=255)

     gCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGt                                                                <  1:704888/71‑1 (MQ=255)

       aGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCa                                                                    >  1:3168320/1‑65 (MQ=255)

        ggTCGCCGGGGAGGATGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTaa                                                             <  1:1150468/71‑1 (MQ=255)

         gTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAAc                                                            <  1:3941710/71‑1 (MQ=255)

            gCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATg                                                         <  1:2939025/71‑1 (MQ=255)

            gCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATg                                                         <  1:4475722/71‑1 (MQ=255)

            gCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATg                                                         <  1:3649621/71‑1 (MQ=255)

               ggggAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGgc                                                       >  1:1823195/1‑70 (MQ=255)

                 ggaggaGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGgcgcg                                                    >  1:1495798/1‑71 (MQ=255)

                   aggaggTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACAt                                                          <  1:3100342/63‑1 (MQ=255)

                      aggTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTAtt                                               >  1:4231353/1‑71 (MQ=255)

                      aggTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGAGCTAtt                                               >  1:3844417/1‑71 (MQ=255)

                      aggTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTAtt                                               <  1:41619/71‑1 (MQ=255)

                       ggTATGATAAGCACCGTCGCATTATTCTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTAttt                                              <  1:4362680/71‑1 (MQ=255)

                        gTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTAttt                                              <  1:2823284/70‑1 (MQ=255)

                        gTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTAttt                                              <  1:3913248/70‑1 (MQ=255)

                        gTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTAttt                                              <  1:3141313/70‑1 (MQ=255)

                        gTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTAttt                                              <  1:3082478/70‑1 (MQ=255)

                        gTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTAttt                                              <  1:1993955/70‑1 (MQ=255)

                          aTGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTtct                                            >  1:2602106/1‑70 (MQ=255)

                           tGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCtca                                          <  1:2459954/71‑1 (MQ=255)

                            gATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTaa                                                             <  1:4574915/51‑1 (MQ=255)

                            gATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGgcgcgc                                                   <  1:397494/61‑1 (MQ=255)

                            gATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGgcgcgc                                                   <  1:4071931/61‑1 (MQ=255)

                            gATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCtcat                                         >  1:4341451/1‑71 (MQ=255)

                            gATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCtcat                                         >  1:590313/1‑71 (MQ=255)

                            gATAAGAACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGgcgcgc                                                   <  1:1686343/61‑1 (MQ=255)

                             aTAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGttt                                                                       >  1:1152582/1‑40 (MQ=255)

                             aTAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCa                                                                    >  1:379477/1‑43 (MQ=255)

                             aTAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACa                                                           >  1:712891/1‑52 (MQ=255)

                             aTAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGgcgc                                                     >  1:484582/1‑58 (MQ=255)

                             aTAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGgcgc                                                     >  1:3094702/1‑58 (MQ=255)

                             aTAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGgcg                                                      >  1:2762519/1‑57 (MQ=255)

                              tAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTa                                                              <  1:61033/48‑1 (MQ=255)

                              tAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTa                                                              <  1:617763/48‑1 (MQ=255)

                              tAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTa                                                              <  1:709791/48‑1 (MQ=255)

                              tAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACa                                                           >  1:2014679/1‑51 (MQ=255)

                                aGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCAc                                      >  1:3376771/1‑70 (MQ=255)

                                aGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCAc                                      <  1:3269238/70‑1 (MQ=255)

                                aGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCAc                                      <  1:2600549/70‑1 (MQ=255)

                                aGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCAc                                      <  1:2220569/70‑1 (MQ=255)

                                  cACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTa                                    >  1:1140202/1‑70 (MQ=255)

                                     cGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTAcgcg                                >  1:3716225/1‑71 (MQ=255)

                                      gTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTAcgcgc                               >  1:2695303/1‑71 (MQ=255)

                                       tCGCATTATTTTGGGCTTTATGTGTCGTTTGCAtt                                                                  <  1:621678/35‑1 (MQ=255)

                                         gCATTATTTTGGGCTTTATGTGTCGTTTGCATTGt                                                                >  1:4411437/1‑35 (MQ=255)

                                         gCATTATTTTGGGCTTTATGTGTCGTTTGCATTGt                                                                >  1:3066167/1‑35 (MQ=255)

                                         gCATTATTTTGGGCTTTATGTGTCGTTTGCATTGt                                                                >  1:3471892/1‑35 (MQ=255)

                                          cATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttg                           <  1:1847372/71‑1 (MQ=255)

                                           attattTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttgt                          <  1:537777/71‑1 (MQ=255)

                                           attattTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttgt                          <  1:2200962/71‑1 (MQ=255)

                                           attattTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCtca                                          <  1:4171367/55‑1 (MQ=255)

                                           attattTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttgt                          <  1:1845271/71‑1 (MQ=255)

                                            ttattTTTGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttgtt                         <  1:1903260/71‑1 (MQ=255)

                                            ttattTTGGGCTTTATGTGTCGTTTGCATTTTTAACATGGCGCGCTATTTCTCATCACTa                                    <  1:1614093/60‑1 (MQ=255)

                                            ttattTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTa                                    <  1:2268498/60‑1 (MQ=255)

                                            ttattTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTa                                    <  1:1939821/60‑1 (MQ=255)

                                            ttattTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttgtt                         <  1:858640/71‑1 (MQ=255)

                                             tattTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTAGGCGCgttgtt                         <  1:3364387/70‑1 (MQ=255)

                                             tattTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttgtt                         <  1:1382189/70‑1 (MQ=255)

                                             tattTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttgt                          >  1:1418480/1‑69 (MQ=255)

                                             tattTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttgt                          >  1:4528177/1‑69 (MQ=255)

                                              attTTGGGCTTTATGTGTCGTTTGCATTGTTAACa                                                           >  1:1206025/1‑35 (MQ=255)

                                              attTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTAcg                                  >  1:1639777/1‑60 (MQ=255)

                                              attTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTa                                    >  1:4022085/1‑58 (MQ=255)

                                                    ggCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTAc                 <  1:2883911/71‑1 (MQ=255)

                                                     gCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACt                <  1:2699538/71‑1 (MQ=255)

                                                      cTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTGATCACTACGCGCGTTGTTAGTGGTACTg               >  1:1401170/1‑71 (MQ=255)

                                                      cTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTg               >  1:1106465/1‑71 (MQ=255)

                                                      cTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTg               >  1:2628150/1‑71 (MQ=255)

                                                        ttATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCtcat                                         <  1:2400953/43‑1 (MQ=255)

                                                        ttATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCtcat                                         <  1:459902/43‑1 (MQ=255)

                                                        ttATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCg             >  1:1594219/1‑71 (MQ=255)

                                                         tATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCg             >  1:456655/1‑70 (MQ=255)

                                                          aTGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGt            >  1:2886211/1‑70 (MQ=255)

                                                           tgtgTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTa           <  1:4400157/70‑1 (MQ=255)

                                                             tgtCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTAcgcgc                               <  1:1859372/48‑1 (MQ=255)

                                                             tgtCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTAcgcgc                               <  1:2593608/48‑1 (MQ=255)

                                                             tgtCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTAc                 >  1:420523/1‑62 (MQ=255)

                                                             tgtCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTAc                 >  1:863874/1‑62 (MQ=255)

                                                             tgtCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTAc                 >  1:1080752/1‑62 (MQ=255)

                                                               tCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTg       <  1:3893072/70‑1 (MQ=255)

                                                                cGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGc      >  1:543716/1‑70 (MQ=255)

                                                                 gTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttgtt                         <  1:4412603/50‑1 (MQ=255)

                                                                 gTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCg     <  1:4606365/70‑1 (MQ=255)

                                                                 gTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCg     <  1:1607173/70‑1 (MQ=255)

                                                                 gTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGa    >  1:2872250/1‑71 (MQ=255)

                                                                 gTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGa    >  1:2898839/1‑71 (MQ=255)

                                                                 gTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGa    >  1:182782/1‑71 (MQ=255)

                                                                 gTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGa    >  1:1663187/1‑71 (MQ=255)

                                                                 gTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGa    <  1:4034060/71‑1 (MQ=255)

                                                                 gTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGa    >  1:4053040/1‑71 (MQ=255)

                                                                  tttGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGAt   >  1:2893718/1‑71 (MQ=255)

                                                                   ttGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc  >  1:2920243/1‑71 (MQ=255)

                                                                   ttGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc  >  1:370315/1‑71 (MQ=255)

CGCGGGCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATC  >  minE/2605786‑2605923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A