Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,390,615 |
A→G |
7.6% |
Q249Q (CAA→CAG) |
nfo → |
endonuclease IV with intrinsic 3'‑5' exonuclease activity |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,390,615 | 0 | A | G | 7.6%
| 386.1
/ 17.7
| 132 | Q249Q (CAA→CAG) | nfo | endonuclease IV with intrinsic 3'‑5' exonuclease activity |
| Reads supporting (aligned to +/- strand): ref base A (60/62); new base G (2/8); total (62/70) |
| Fisher's exact test for biased strand distribution p-value = 1.02e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 |
Only 100 of 138 total aligned reads displayed.
GCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATC > minE/1390545‑1390684
|
gCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCaa < 1:1097718/71‑1 (MQ=255)
ccGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAg < 1:2729056/71‑1 (MQ=255)
ccGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAg < 1:2090965/71‑1 (MQ=255)
ccGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAg < 1:3685389/71‑1 (MQ=255)
gTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAgacga < 1:2706011/71‑1 (MQ=255)
gTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAgac > 1:1360636/1‑69 (MQ=255)
gACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCg > 1:3123553/1‑71 (MQ=255)
gACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCg > 1:2959623/1‑71 (MQ=255)
aCCGCCATCATAGCCTCGGTGAAGGCAATATCGTTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGt > 1:2928315/1‑71 (MQ=255)
aCCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGt > 1:1582249/1‑71 (MQ=255)
aCCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGt < 1:3113875/71‑1 (MQ=255)
cGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGttt > 1:3448315/1‑71 (MQ=255)
cGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGttt > 1:519994/1‑71 (MQ=255)
cGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGtt > 1:3162511/1‑70 (MQ=255)
gCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTc < 1:1253868/71‑1 (MQ=255)
gCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTc < 1:672887/71‑1 (MQ=255)
ccATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTc > 1:1486726/1‑70 (MQ=255)
ccATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCg < 1:1176563/71‑1 (MQ=255)
ccATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCg < 1:1640257/71‑1 (MQ=255)
ccATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCg < 1:520104/71‑1 (MQ=255)
catcatAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGa < 1:1814213/71‑1 (MQ=255)
atcatAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGAc < 1:3187645/71‑1 (MQ=255)
atcatAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGAc < 1:2572562/71‑1 (MQ=255)
catAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACgg > 1:2677675/1‑71 (MQ=255)
tAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCa < 1:2768596/71‑1 (MQ=255)
gcgtCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCAt < 1:403662/67‑1 (MQ=255)
gCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCAt < 1:2474625/70‑1 (MQ=255)
gCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCAt < 1:2700673/70‑1 (MQ=255)
gCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCAt < 1:3076411/70‑1 (MQ=255)
gCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCAt < 1:2016601/70‑1 (MQ=255)
ccTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGa < 1:1876822/63‑1 (MQ=255)
ccTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGa < 1:1217558/63‑1 (MQ=255)
ccTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTc > 1:2551911/1‑71 (MQ=255)
cTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCg < 1:2161016/61‑1 (MQ=255)
tCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGAc > 1:2113032/1‑62 (MQ=255)
tCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACg > 1:1602330/1‑63 (MQ=255)
tCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCg > 1:389904/1‑71 (MQ=255)
tCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCg > 1:297626/1‑71 (MQ=255)
tCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCg > 1:605427/1‑71 (MQ=255)
tCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCg > 1:856022/1‑71 (MQ=255)
ggTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCAtt > 1:2971645/1‑66 (MQ=255)
ggTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCg < 1:801347/69‑1 (MQ=255)
ggTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCt < 1:1493583/71‑1 (MQ=255)
gTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCt < 1:1115071/70‑1 (MQ=255)
gTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTg < 1:3409718/71‑1 (MQ=255)
gTGAAGG‑AATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCg < 1:2284072/56‑1 (MQ=255)
tGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGa < 1:1215368/71‑1 (MQ=255)
gAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCg < 1:3547037/55‑1 (MQ=255)
aGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATcc < 1:2660079/71‑1 (MQ=255)
aGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATca < 1:293695/71‑2 (MQ=255)
ggCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCt < 1:142821/71‑1 (MQ=255)
ggCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGtt > 1:1613684/1‑49 (MQ=255)
ggCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCa > 1:1629714/1‑58 (MQ=255)
gCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTc < 1:703883/71‑1 (MQ=255)
cAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTc > 1:2016046/1‑70 (MQ=255)
aaTATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGa < 1:1017209/71‑1 (MQ=255)
tatCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCAt < 1:906043/54‑1 (MQ=255)
tatCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGt < 1:3703413/43‑1 (MQ=255)
atCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAAc > 1:2419125/1‑71 (MQ=255)
atCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTc > 1:2534242/1‑66 (MQ=255)
tCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAAcc < 1:2673935/71‑1 (MQ=255)
cGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAAcc < 1:1336291/70‑1 (MQ=255)
ggTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCAt < 1:1770976/71‑1 (MQ=255)
gTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGa > 1:1161223/1‑43 (MQ=255)
gTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAAc > 1:1374109/1‑67 (MQ=255)
tCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGa > 1:1746374/1‑56 (MQ=255)
tCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCa < 1:2525183/71‑1 (MQ=255)
cATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCg > 1:3210654/1‑51 (MQ=255)
atgCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCg < 1:2934682/71‑1 (MQ=255)
tgCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCgg < 1:397683/71‑1 (MQ=255)
tgCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCgg < 1:937381/71‑1 (MQ=255)
cGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGa > 1:1410493/1‑70 (MQ=255)
ttCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCgg < 1:767221/67‑1 (MQ=255)
ttCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGatat < 1:715274/71‑1 (MQ=255)
ccGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGc > 1:85562/1‑41 (MQ=255)
ccGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGc > 1:35051/1‑41 (MQ=255)
ccGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGc > 1:775101/1‑41 (MQ=255)
ccGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGc > 1:1523469/1‑41 (MQ=255)
ccGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGc > 1:1307764/1‑41 (MQ=255)
ccGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGa > 1:2424908/1‑66 (MQ=255)
ccGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATc < 1:2777701/70‑1 (MQ=255)
cGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCt < 1:3243179/70‑1 (MQ=255)
cGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCt < 1:772573/70‑1 (MQ=255)
cGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCt < 1:1733489/70‑1 (MQ=255)
cGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCt < 1:839473/70‑1 (MQ=255)
cGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTg > 1:1444903/1‑71 (MQ=255)
cGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTg > 1:258848/1‑71 (MQ=255)
gCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCa < 1:2498410/57‑1 (MQ=255)
ggATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTggg > 1:602820/1‑69 (MQ=255)
gATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTggg > 1:1676911/1‑68 (MQ=255)
tCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCagaa < 1:3368714/71‑1 (MQ=255)
tCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCag > 1:1860340/1‑69 (MQ=255)
tCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCag > 1:3043975/1‑69 (MQ=255)
tCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTCGGCag > 1:800313/1‑69 (MQ=255)
gCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATc > 1:1383369/1‑46 (MQ=255)
gCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCagaa > 1:1047062/1‑67 (MQ=255)
cAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAgaga < 1:52554/70‑1 (MQ=255)
cAAGACGACCGTTTCGACGGCATTC‑‑CTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATc < 1:1048384/70‑1 (MQ=255)
aGACGACCGTTTCGACGGCATTCCGCTGATCCTCGaa < 1:165957/37‑1 (MQ=255)
aGACGACCGTTTCGACGGCATTCCGCTGATCCTCGaa < 1:1111864/37‑1 (MQ=255)
|
GCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATC > minE/1390545‑1390684
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
