Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,523,621 |
C→T |
71.0% |
G32S (GGT→AGT) |
dinD ← |
DNA‑damage‑inducible protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,523,621 | 0 | C | T | 71.0%
| 102.2
/ 52.0
| 69 | G32S (GGT→AGT) | dinD | DNA‑damage‑inducible protein |
| Reads supporting (aligned to +/- strand): ref base C (6/14); new base T (31/18); total (37/32) |
| Fisher's exact test for biased strand distribution p-value = 1.68e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACCCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCATTAATCAGTTTTATCTCTT > minE/2523554‑2523689
|
ccACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATGCAGGTATAAAGTGACGATACTGTGAATAACTCag > 1:2324409/1‑71 (MQ=255)
ccACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCag > 1:837556/1‑71 (MQ=255)
ccACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCag > 1:2280899/1‑71 (MQ=255)
ccACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCag > 1:2960825/1‑71 (MQ=255)
ccACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCag > 1:806298/1‑71 (MQ=255)
aCTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCag > 1:431958/1‑69 (MQ=255)
cTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCagta > 1:581922/1‑70 (MQ=255)
ttCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACCCAGTAGttt < 1:1064709/71‑1 (MQ=255)
ttCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACCCAGTAGttt < 1:1467708/71‑1 (MQ=255)
ttCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACCCAGTAGtt < 1:2914408/70‑1 (MQ=255)
ctcACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACCCAGTAGTTTcc > 1:2312310/1‑71 (MQ=255)
caGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAg > 1:2563758/1‑71 (MQ=255)
cttcttTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCagtagt < 1:2884031/59‑1 (MQ=255)
cttTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACCCAGTAGTTTCCCAAGTTGTc < 1:1569145/69‑1 (MQ=255)
tttGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCt < 1:3674503/69‑1 (MQ=255)
tGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGt > 1:2514407/1‑65 (MQ=255)
ggCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACCCAGTAGTTTCCCAAGTTGTCTTGCt > 1:2494199/1‑70 (MQ=255)
gcgTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCAt > 1:3725351/1‑71 (MQ=255)
gcgTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCAt < 1:681036/71‑1 (MQ=255)
gcgTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCAt < 1:1248674/71‑1 (MQ=255)
cgTTAATACAGGTATAAAGTGACGATACTCTGAATAACCCAGTAGTTTCCCAAGTTGTCTTGCTGACCAtt < 1:2831537/71‑1 (MQ=255)
gTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCagtag < 1:2979419/44‑1 (MQ=255)
gTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCagtag < 1:2343150/44‑1 (MQ=255)
gTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCagtag < 1:1040900/44‑1 (MQ=255)
gTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCagtag < 1:2202235/44‑1 (MQ=255)
ttAATACAGGTATAAAGTGACGATACTCTGAATAACCCagt < 1:556554/41‑1 (MQ=255)
aaTACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGtt < 1:1147867/70‑1 (MQ=255)
aaTACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTc > 1:2500943/1‑71 (MQ=255)
aaTACAGGTATAAAGTGACGATACTCTGAATAACCCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTg > 1:90895/1‑68 (MQ=255)
aaTACAGGTATAAAGTGACGATACTCTGAATAACCCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGtt < 1:3135897/70‑1 (MQ=255)
aaTACAGGTATAAAGTGACGATACTCTGAATAACCCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGtt < 1:2854430/70‑1 (MQ=255)
aTACAGGTATAAAGTGACGATACTCTGAATAACCCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGtt < 1:1155778/69‑1 (MQ=255)
cAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCt > 1:155570/1‑71 (MQ=255)
cAGGTATAAAGTGACGATACTCTGAATAACCCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGc < 1:2816591/70‑1 (MQ=255)
ggTATAAAGTGACGATACTCTGAATAACCCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTcc > 1:2894489/1‑71 (MQ=255)
taAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCtt < 1:2201582/47‑1 (MQ=255)
taAAGTGACGATACTCTGAATAACCCAGTAGTTTCCCAAGTTGTCTTGCt < 1:1715478/50‑1 (MQ=255)
aaaGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGc > 1:2332736/1‑48 (MQ=255)
aaaGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGc > 1:394971/1‑48 (MQ=255)
aaGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCt > 1:2540686/1‑44 (MQ=255)
aaGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCt > 1:1057492/1‑44 (MQ=255)
aaGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCt > 1:630101/1‑44 (MQ=255)
aaGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCt > 1:2989914/1‑44 (MQ=255)
aGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCa > 1:2185977/1‑71 (MQ=255)
gTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCAt > 1:1736119/1‑71 (MQ=255)
gTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCAt > 1:2013025/1‑71 (MQ=255)
tgccGAAACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCa > 1:8561/4‑69 (MQ=255)
tGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGt < 1:1875701/54‑1 (MQ=255)
tGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGt < 1:1720011/54‑1 (MQ=255)
tGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGc < 1:522853/59‑1 (MQ=255)
tGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGc < 1:1639329/59‑1 (MQ=255)
gACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCAtt > 1:1453923/1‑70 (MQ=255)
gACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCAtt > 1:1378911/1‑70 (MQ=255)
gACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCAt > 1:3619432/1‑69 (MQ=255)
aCGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTc > 1:570173/1‑59 (MQ=255)
aCGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCATTa < 1:3069784/70‑1 (MQ=255)
gATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTg > 1:3397890/1‑63 (MQ=255)
ctctGAATAACCCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCATTAATCAGt < 1:2626431/70‑1 (MQ=255)
tctGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTg < 1:2367939/44‑1 (MQ=255)
tctGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTg < 1:3042568/44‑1 (MQ=255)
tctGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTg < 1:232513/44‑1 (MQ=255)
tctGAATAACCCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTg < 1:2765517/44‑1 (MQ=255)
tctGAATAACCCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTg < 1:2630760/44‑1 (MQ=255)
tGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGtt > 1:2194078/1‑44 (MQ=255)
tAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGtt > 1:1015319/1‑50 (MQ=255)
tAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCATTAATCAGTTTTAtctc > 1:3733987/1‑71 (MQ=255)
tAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCATTAATCAGTTTTAtctc > 1:2795224/1‑71 (MQ=255)
aCCCAGTAGTTTCCCAAGTTTTCTTGCTGACCATTGTTCTGCTCCGTTTGCATTAATCAGTTTTATCTCtt > 1:902543/1‑71 (MQ=255)
aCCCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCATTAATCAGTTTTATCTCtt > 1:10991/1‑71 (MQ=255)
|
CCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACCCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCATTAATCAGTTTTATCTCTT > minE/2523554‑2523689
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
