Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,526,945 |
C→T |
62.7% |
E13K (GAG→AAG) |
ttk ← |
division inhibitor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,526,945 | 0 | C | T | 62.7%
| 46.4
/ 61.6
| 59 | E13K (GAG→AAG) | ttk | division inhibitor |
| Reads supporting (aligned to +/- strand): ref base C (15/7); new base T (13/24); total (28/31) |
| Fisher's exact test for biased strand distribution p-value = 1.74e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01 |
GCCGTCGTGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATAAGCACTT > minE/2526878‑2527011
|
gCCGTCGTGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTtgc < 1:3195920/70‑1 (MQ=255)
gCCGTCGTGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTcgc < 1:887072/70‑1 (MQ=255)
gCCGTCGTGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTcgc < 1:1160287/70‑1 (MQ=255)
gtcgtGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACg > 1:957764/1‑71 (MQ=255)
gtcgtGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACg > 1:2654009/1‑71 (MQ=255)
tcgtGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACgg > 1:1092342/1‑71 (MQ=255)
tcgtGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACgg > 1:3430017/1‑71 (MQ=255)
tcgtGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACgg > 1:2799708/1‑71 (MQ=255)
tcgtGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACgg > 1:3357788/1‑71 (MQ=255)
cgtGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGt > 1:3476055/1‑71 (MQ=255)
cgtGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGt > 1:2960472/1‑71 (MQ=255)
gtGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGtt > 1:824244/1‑71 (MQ=255)
gtGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGt < 1:3439298/70‑1 (MQ=255)
gtGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGt < 1:941603/70‑1 (MQ=255)
aTACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTcgc < 1:2004091/61‑1 (MQ=255)
aTACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTcgc < 1:163281/61‑1 (MQ=255)
aTACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCt > 1:2211026/1‑71 (MQ=255)
cGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCttt < 1:1557918/70‑1 (MQ=255)
cGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCttt < 1:1781739/70‑1 (MQ=255)
ttGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCg < 1:1412364/71‑1 (MQ=255)
cTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgt < 1:117116/71‑1 (MQ=255)
cTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgt > 1:1916627/1‑71 (MQ=255)
cTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgt < 1:2074228/71‑1 (MQ=255)
cATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAgtttgt < 1:2614003/71‑1 (MQ=255)
cATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAgtttg < 1:2649733/70‑1 (MQ=255)
aTCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCa > 1:2078079/1‑64 (MQ=255)
tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAgtttgttt < 1:904327/71‑1 (MQ=255)
tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAgtttgttt < 1:1188105/71‑1 (MQ=255)
tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAgtttgttt < 1:1413189/71‑1 (MQ=255)
gCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGtttt < 1:582727/70‑1 (MQ=255)
cTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTc > 1:147066/1‑71 (MQ=255)
cTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTc > 1:573738/1‑71 (MQ=255)
cTGGATTCCAGCATCAGAGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTc < 1:2004282/71‑1 (MQ=255)
tGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTtgc < 1:1745243/42‑1 (MQ=255)
tGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTc < 1:1051137/70‑1 (MQ=255)
gATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGc < 1:2293376/71‑1 (MQ=255)
ttCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCATTTCGCAGTTTGTTTTTCTGCCa > 1:1197096/1‑71 (MQ=255)
ccAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTc > 1:3659298/1‑50 (MQ=255)
ccAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTcc > 1:2408431/1‑45 (MQ=255)
aGCATCAGCGCCAGAGCCTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGt > 1:1790786/1‑70 (MQ=255)
cATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCtt < 1:3447283/43‑1 (MQ=255)
cATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTa > 1:780626/1‑70 (MQ=255)
ccAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAgt < 1:3087082/43‑1 (MQ=255)
cAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATAcg < 1:1686168/70‑2 (MQ=255)
cAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATAccc < 1:2607271/71‑1 (MQ=255)
cAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATAccc < 1:1094081/71‑1 (MQ=255)
gagaCTGAAGTATTTCCTTGCGACGGTTCCTTTTCGc > 1:497423/1‑37 (MQ=255)
gagaCTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGGCATGTTACAAAATAccc < 1:3072212/69‑1 (MQ=255)
gagaCTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCt > 1:1682802/1‑71 (MQ=255)
gagaCTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCt > 1:2034655/1‑71 (MQ=255)
gagaCTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGACATGTTACAAAATAccc > 1:145442/1‑69 (MQ=255)
agaCTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGGTTGttttt < 1:2731384/47‑1 (MQ=255)
gaCTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTg > 1:317030/1‑70 (MQ=255)
cTGAAGTATTTCCTCGCGGCGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGaaa < 1:3591955/71‑1 (MQ=255)
aaGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAAt < 1:1554937/70‑1 (MQ=255)
aaGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATa < 1:781019/71‑1 (MQ=255)
aaGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATa < 1:125599/71‑1 (MQ=255)
aaGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAAt > 1:907378/1‑70 (MQ=255)
aGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATaa < 1:2869149/71‑1 (MQ=255)
tATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACaa < 1:394307/52‑1 (MQ=255)
tATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACaa < 1:3722103/52‑1 (MQ=255)
tttCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATAAGCAc > 1:1153279/1‑71 (MQ=255)
tCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATAAGCACt < 1:275252/70‑1 (MQ=255)
tCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATAAGCACtt > 1:3680184/1‑71 (MQ=255)
tCCTCGCGACGGTTCCTTTTCGCAGTTTGTATTTCTGCCATGTTACAAAATACACCTGAAAATAAGCACt > 1:661454/1‑70 (MQ=255)
|
GCCGTCGTGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATAAGCACTT > minE/2526878‑2527011
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
