Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F17 I0 R2 159 90.8 4763082 92.7% 4415377 60.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,526,945 C→T 8.4% E13K (GAG→AAG)  ttk ← division inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,526,9450CT 8.4% 297.2 / 12.9 95E13K (GAG→AAG) ttkdivision inhibitor
Reads supporting (aligned to +/- strand):  ref base C (48/38);  new base T (2/6);  total (51/44)
Fisher's exact test for biased strand distribution p-value = 1.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGTCGTGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATAAGCACTT  >  minE/2526878‑2527011
                                                                   |                                                                  
gCCGTCGTGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTcgc                                                                  <  1:962632/70‑1 (MQ=255)
   gtcgtGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACg                                                              >  1:1285826/1‑71 (MQ=255)
    tcgtGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACgg                                                             >  1:532681/1‑71 (MQ=255)
    tcgtGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTAGCGACg                                                              >  1:2584043/1‑70 (MQ=255)
     cgtGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTcg                                                                   >  1:1193401/1‑64 (MQ=255)
      gtGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGt                                                            <  1:2758625/70‑1 (MQ=255)
          tACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCtt                                                       >  1:1800783/1‑71 (MQ=255)
          tACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCtt                                                       >  1:4744467/1‑71 (MQ=255)
          tACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCtt                                                       >  1:7802/1‑71 (MQ=255)
          tACGTTGGCTTCCATCGCTGGATTCCAGAATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCtt                                                       <  1:3194507/71‑1 (MQ=255)
            cGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACg                                                              <  1:4352532/62‑1 (MQ=255)
            cGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCttt                                                      <  1:3431908/70‑1 (MQ=255)
                ggCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCg                                                   >  1:3018574/1‑69 (MQ=255)
                 gCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAg                                                <  1:2661752/71‑1 (MQ=255)
                     ccATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAgtttg                                            >  1:1060711/1‑71 (MQ=255)
                     ccATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAgttt                                             >  1:1041672/1‑70 (MQ=255)
                     ccATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTcc                                                         >  1:4074351/1‑58 (MQ=255)
                     ccATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttg                                            >  1:4068764/1‑71 (MQ=255)
                     ccATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttg                                            >  1:4608987/1‑71 (MQ=255)
                      cATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgt                                           <  1:1734409/71‑1 (MQ=255)
                       aTCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGa                                                                >  1:2233394/1‑49 (MQ=255)
                       aTCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGa                                                                >  1:940453/1‑49 (MQ=255)
                       aTCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGa                                                                >  1:2639744/1‑49 (MQ=255)
                       aTCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCa                                                 >  1:2275305/1‑64 (MQ=255)
                       aTCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAg                                                >  1:2085002/1‑65 (MQ=255)
                        tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGa                                                                >  1:1925519/1‑48 (MQ=255)
                        tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGa                                                                >  1:4427027/1‑48 (MQ=255)
                        tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGa                                                                >  1:4715429/1‑48 (MQ=255)
                        tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGa                                                                >  1:2734439/1‑48 (MQ=255)
                        tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGa                                                                >  1:1609454/1‑48 (MQ=255)
                        tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         <  1:4367988/71‑1 (MQ=255)
                        tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         <  1:7312/71‑1 (MQ=255)
                        tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         >  1:3464937/1‑71 (MQ=255)
                        tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         <  1:3768998/71‑1 (MQ=255)
                        tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         <  1:2527534/71‑1 (MQ=255)
                        tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         <  1:1047476/71‑1 (MQ=255)
                        tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         >  1:1145110/1‑71 (MQ=255)
                        tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         <  1:1966736/71‑1 (MQ=255)
                        tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         <  1:1486042/71‑1 (MQ=255)
                         cGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGtttt                                        >  1:2374243/1‑71 (MQ=255)
                         cGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGtttt                                        >  1:4237596/1‑71 (MQ=255)
                         cGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGtttt                                        >  1:2183139/1‑71 (MQ=255)
                         cGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGtttt                                        >  1:4602865/1‑71 (MQ=255)
                          gCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGttttt                                       <  1:2955906/71‑1 (MQ=255)
                          gCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGttttt                                       <  1:2179143/71‑1 (MQ=255)
                          gCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGttttt                                       >  1:1297243/1‑71 (MQ=255)
                          gCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGttttt                                       <  1:3262430/71‑1 (MQ=255)
                          gCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGtttt                                        >  1:376724/1‑70 (MQ=255)
                          gCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGtttt                                        <  1:4392501/70‑1 (MQ=255)
                          gCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGtttt                                        <  1:2263185/70‑1 (MQ=255)
                                  ccAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCAt                                <  1:4427608/70‑1 (MQ=255)
                                  ccAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATg                               >  1:3790523/1‑71 (MQ=255)
                                  ccAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATg                               >  1:3796166/1‑71 (MQ=255)
                                   cAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGttttt                                       <  1:1324183/62‑1 (MQ=255)
                                   cAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGt                              <  1:2685175/71‑1 (MQ=255)
                                    tgCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGtttt                                        <  1:1145915/59‑1 (MQ=255)
                                     gCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTa                            <  1:1974690/71‑1 (MQ=255)
                                     gCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCt                                     >  1:3525384/1‑62 (MQ=255)
                                     gCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTa                            >  1:1810528/1‑71 (MQ=255)
                                       aTCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTAc                           >  1:1780927/1‑70 (MQ=255)
                                       aTCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTAc                           >  1:3554421/1‑70 (MQ=255)
                                       aTCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACa                          <  1:3154266/71‑1 (MQ=255)
                                        tCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         <  1:3489167/55‑1 (MQ=255)
                                        tCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         <  1:4087868/55‑1 (MQ=255)
                                        tCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         <  1:4027972/55‑1 (MQ=255)
                                        tCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         <  1:2175740/55‑1 (MQ=255)
                                        tCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACaa                         <  1:3458465/71‑1 (MQ=255)
                                        tCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACaa                         <  1:3456363/71‑1 (MQ=255)
                                        tCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACaa                         <  1:4615662/71‑1 (MQ=255)
                                        tCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACaa                         <  1:1472561/71‑1 (MQ=255)
                                          aGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACaaa                        <  1:3692048/70‑1 (MQ=255)
                                           gcgcCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCa                                                 <  1:2421634/44‑1 (MQ=255)
                                           gcgcCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCa                                                 <  1:1670476/44‑1 (MQ=255)
                                           gcgcCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCa                                                 <  1:3124629/44‑1 (MQ=255)
                                               cAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATAccc                  <  1:2893970/71‑1 (MQ=255)
                                                agagACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGttttt                                       <  1:4069922/49‑1 (MQ=255)
                                                 gagaCTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCt                >  1:2156087/1‑71 (MQ=255)
                                                  agaCTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGttttt                                       >  1:1512486/1‑47 (MQ=255)
                                                  agaCTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGttttt                                       >  1:1051056/1‑47 (MQ=255)
                                                  agaCTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGttttt                                       >  1:4218185/1‑47 (MQ=255)
                                                  agaCTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACaa                         <  1:4012230/61‑1 (MQ=255)
                                                    aCTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGa              >  1:4601623/1‑70 (MQ=255)
                                                    aCTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGa              >  1:2398505/1‑70 (MQ=255)
                                                           tATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATAAGc      <  1:1539343/71‑1 (MQ=255)
                                                            aTTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACaaaa                       >  1:1837029/1‑53 (MQ=255)
                                                             tttCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATAAGCa     <  1:1193602/70‑1 (MQ=255)
                                                             tttCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCt                                     >  1:3736067/1‑38 (MQ=255)
                                                             tttCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCt                                     >  1:563461/1‑38 (MQ=255)
                                                             tttCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACaaaa                       <  1:2215292/52‑1 (MQ=255)
                                                             tttCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATAAGCAc    <  1:2970288/71‑1 (MQ=255)
                                                               tCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACaaaa                       >  1:690714/1‑50 (MQ=255)
                                                               tCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATAAGCACtt  >  1:3527913/1‑71 (MQ=255)
                                                                ccTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCt                                     >  1:2305299/1‑35 (MQ=255)
                                                                ccTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATg                               >  1:1499026/1‑41 (MQ=255)
                                                                ccTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATg                               >  1:3867605/1‑41 (MQ=255)
                                                                ccTCGCGACGGTTACTTTTCGCAGTTTGTTTTTCTGCCATg                               >  1:4077859/1‑41 (MQ=255)
                                                                 cTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACa                          <  1:1338252/45‑1 (MQ=255)
                                                                   |                                                                  
GCCGTCGTGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATAAGCACTT  >  minE/2526878‑2527011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A