Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,550,360 |
A→G |
52.9% |
K77K (AAA→AAG) |
tdh → |
threonine 3‑dehydrogenase, NAD(P)‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,550,360 | 0 | A | G | 52.9%
| 17.6
/ 148.6
| 104 | K77K (AAA→AAG) | tdh | threonine 3‑dehydrogenase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base A (28/21); new base G (28/27); total (56/48) |
| Fisher's exact test for biased strand distribution p-value = 5.59e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 |
Only 100 of 111 total aligned reads displayed.
TCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCGTG > minE/2550293‑2550430
|
tCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGc > 1:3208855/1‑71 (MQ=255)
tCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGc > 1:2109575/1‑71 (MQ=255)
tCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGc > 1:3668368/1‑71 (MQ=255)
tCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGc > 1:3020075/1‑71 (MQ=255)
cccGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGc > 1:2536461/1‑70 (MQ=255)
cccGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGc > 1:2459228/1‑70 (MQ=255)
cccGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGc > 1:2759388/1‑70 (MQ=255)
ccGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGaaa > 1:2794998/1‑66 (MQ=255)
ccGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCt > 1:1479506/1‑70 (MQ=255)
tGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAg < 1:366746/71‑1 (MQ=255)
tGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAg < 1:1779985/71‑1 (MQ=255)
cGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTc > 1:3711232/1‑65 (MQ=255)
tGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCgg > 1:2473631/1‑70 (MQ=255)
ggTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGAt < 1:815201/66‑1 (MQ=255)
ggTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGc < 1:1118491/70‑1 (MQ=255)
tCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGAt > 1:3181797/1‑71 (MQ=255)
tCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGAt > 1:1589485/1‑71 (MQ=255)
tCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGAt > 1:1443340/1‑71 (MQ=255)
tCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGAt > 1:1121668/1‑71 (MQ=255)
ggCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGt > 1:3313833/1‑71 (MQ=255)
gCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGtt < 1:2779441/71‑1 (MQ=255)
ccATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTc < 1:2218290/52‑1 (MQ=255)
ccATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGttt > 1:3030241/1‑71 (MQ=255)
ccATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGttt < 1:1873399/71‑1 (MQ=255)
cATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTc < 1:2376132/71‑1 (MQ=255)
aTGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCt < 1:2905938/71‑1 (MQ=255)
aTGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCt < 1:1551168/71‑1 (MQ=255)
tGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGaaa < 1:2962287/43‑1 (MQ=255)
gAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTgg > 1:2906789/1‑71 (MQ=255)
gAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTgg > 1:2907734/1‑71 (MQ=255)
gAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTgg > 1:2611229/1‑71 (MQ=255)
aaTATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAgg > 1:3112565/1‑43 (MQ=255)
aaTATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTgg < 1:1525019/70‑1 (MQ=255)
aaTATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTgg < 1:483153/70‑1 (MQ=255)
aaTATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGc > 1:1266625/1‑71 (MQ=255)
atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGc > 1:951726/1‑70 (MQ=255)
atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGc > 1:1063634/1‑70 (MQ=255)
atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGc > 1:376162/1‑70 (MQ=255)
atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:349740/71‑1 (MQ=255)
atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:3206729/71‑1 (MQ=255)
atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:278192/71‑1 (MQ=255)
atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:1032341/71‑1 (MQ=255)
atatGTCGGTGAAGTGGTAGGGATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGc > 1:2414956/1‑70 (MQ=255)
tGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGaa < 1:1253636/70‑1 (MQ=255)
tGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGaa < 1:2540188/70‑1 (MQ=255)
gTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCtt > 1:3099619/1‑41 (MQ=255)
gTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCtt > 1:836521/1‑41 (MQ=255)
cGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:2335652/65‑1 (MQ=255)
ggTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTgg < 1:647997/62‑1 (MQ=255)
ggTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGa > 1:3033450/1‑50 (MQ=255)
ggTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGa > 1:2628434/1‑50 (MQ=255)
ggTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGa > 1:2180549/1‑50 (MQ=255)
ggTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCa < 1:1935471/71‑1 (MQ=255)
tGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCa < 1:1910743/69‑1 (MQ=255)
gAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCa < 1:107102/68‑1 (MQ=255)
aGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCa > 1:3112115/1‑71 (MQ=255)
aGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCa > 1:2101227/1‑71 (MQ=255)
aGTGGGAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCg < 1:972241/41‑1 (MQ=38)
gTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGa > 1:2362027/1‑37 (MQ=255)
gTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGAt > 1:2779210/1‑38 (MQ=255)
tGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGaa > 1:1062790/1‑59 (MQ=255)
tGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGaa > 1:2112563/1‑59 (MQ=255)
tGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGGGATCGCGTTTCTGGCGAAGGCCATATCAcc < 1:556714/71‑1 (MQ=255)
gtaggtaTTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCtg > 1:1095541/1‑71 (MQ=255)
gtaggtaTTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCt < 1:3235005/70‑1 (MQ=255)
aggtaTTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCat < 1:1411662/61‑1 (MQ=255)
aggtaTTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCat < 1:2461949/61‑1 (MQ=255)
aggtaTTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCat < 1:1319953/61‑1 (MQ=255)
aggtaTTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCtgtg > 1:1968076/1‑71 (MQ=255)
ggtaTTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGt < 1:172018/44‑1 (MQ=255)
ggtaTTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGt < 1:913117/44‑1 (MQ=255)
ggtaTTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGt < 1:2747493/44‑1 (MQ=255)
ggtaTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCtgtg < 1:2542736/70‑1 (MQ=255)
ggtaTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCtgtg < 1:2864806/70‑1 (MQ=255)
ggtaTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCtgtg < 1:717331/70‑1 (MQ=255)
ggtaTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCtgtg < 1:2417128/70‑1 (MQ=255)
taTTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTc < 1:1438877/71‑1 (MQ=255)
tGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGTCATATCAcc > 1:291177/1‑61 (MQ=255)
tGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGTCATATCACCTGTGGTCAtt > 1:2273538/1‑71 (MQ=255)
tGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGTCATATCACCTGTGGTCAtt > 1:3508681/1‑71 (MQ=255)
tGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGTCATATCACCTGTGGTCAtt > 1:1652872/1‑71 (MQ=255)
tGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGTCATATCACCTGTGGTCAtt > 1:3044974/1‑71 (MQ=255)
ggTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTg > 1:2475803/1‑71 (MQ=255)
ggTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTg > 1:1326206/1‑71 (MQ=255)
ggTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGcc > 1:3247718/1‑52 (MQ=255)
gTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTg < 1:2901054/70‑1 (MQ=255)
gTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGc < 1:2671026/71‑1 (MQ=255)
tCAGGAAGTGAAGGGCTTCAAGATCGGAGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGcc < 1:3462161/71‑1 (MQ=255)
tCAGGAAGTGAAAGGCTTCAAGATCGGCGATcgcg > 1:401675/1‑35 (MQ=255)
tCAGGAAGTGAAAGGCTTCAAGATCGGCGATcgcg > 1:3065968/1‑35 (MQ=255)
tCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGcc < 1:3095215/50‑1 (MQ=255)
tCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGcc > 1:2797887/1‑71 (MQ=255)
cAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCg > 1:378391/1‑71 (MQ=255)
aGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGc < 1:1671248/71‑1 (MQ=255)
ggAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGc > 1:1892454/1‑70 (MQ=255)
gAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:948385/35‑1 (MQ=255)
aaaGGCTTCAAGATCGGCGATCGCGTTTCTGGCGaa > 1:896292/1‑36 (MQ=255)
aaaGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCg > 1:475498/1‑71 (MQ=255)
aaaGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCg > 1:2746950/1‑71 (MQ=255)
aGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCgtg > 1:1022705/1‑71 (MQ=255)
|
TCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCGTG > minE/2550293‑2550430
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
