Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F17 I0 R2
|
159 |
90.8 |
4763082 |
92.7% |
4415377 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,550,360 |
A→G |
5.2% |
K77K (AAA→AAG) |
tdh → |
threonine 3‑dehydrogenase, NAD(P)‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,550,360 | 0 | A | G | 5.2%
| 355.6
/ 8.1
| 115 | K77K (AAA→AAG) | tdh | threonine 3‑dehydrogenase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base A (46/63); new base G (2/4); total (48/67) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01 |
Only 100 of 131 total aligned reads displayed.
CCATCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCGTG > minE/2550290‑2550430
|
ccATCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGaaa < 1:682186/71‑1 (MQ=255)
cATCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGaaa < 1:3125199/70‑1 (MQ=255)
tCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGc > 1:801471/1‑71 (MQ=255)
tCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGc > 1:528096/1‑71 (MQ=255)
tCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGc > 1:4342717/1‑71 (MQ=255)
ccGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCtt < 1:1642766/71‑1 (MQ=255)
ccGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCt < 1:3003499/70‑1 (MQ=255)
cGGTGCCGATGGTCGTGTGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTc < 1:2977184/71‑1 (MQ=255)
ccGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTc > 1:3066377/1‑66 (MQ=255)
ccGATGGTCGT‑GGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGAt < 1:2151049/70‑1 (MQ=255)
aTGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCgg > 1:957461/1‑71 (MQ=255)
aTGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCgg > 1:1364808/1‑71 (MQ=255)
tGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGc > 1:4652203/1‑71 (MQ=255)
ggTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGc > 1:3393361/1‑70 (MQ=255)
gTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGa < 1:2646126/71‑1 (MQ=255)
tCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGAt > 1:2743746/1‑64 (MQ=255)
tCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGAt > 1:1942026/1‑64 (MQ=255)
cGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGAt > 1:306313/1‑70 (MQ=255)
cGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATc > 1:2263092/1‑71 (MQ=255)
cGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATc > 1:2595138/1‑71 (MQ=255)
gTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGTAAGTGAAAGGCTTCAAGATCGGCGATcg < 1:2116906/71‑1 (MQ=255)
gTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATcg > 1:2823804/1‑71 (MQ=255)
gTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATcg < 1:2956457/71‑1 (MQ=255)
gTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATcg < 1:2966847/71‑1 (MQ=255)
gTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATcg < 1:1671984/71‑1 (MQ=255)
gTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATcg < 1:516544/71‑1 (MQ=255)
gTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATcg < 1:3342940/71‑1 (MQ=255)
cATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCaa > 1:4102270/1‑53 (MQ=255)
cATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCaa > 1:1153003/1‑53 (MQ=255)
cATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTc < 1:4536235/71‑1 (MQ=255)
cATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTc < 1:4385435/71‑1 (MQ=255)
cATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTc < 1:4608995/71‑1 (MQ=255)
cATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTc < 1:667324/71‑1 (MQ=255)
cATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTc < 1:1995717/71‑1 (MQ=255)
cATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTc < 1:2854795/71‑1 (MQ=255)
aTGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTc < 1:1152654/70‑1 (MQ=255)
tGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTg > 1:2715460/1‑71 (MQ=255)
gAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGCTCGCGTTTCTg > 1:3272736/1‑70 (MQ=255)
gAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGAt < 1:1577877/60‑1 (MQ=255)
gAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGAt < 1:2482900/60‑1 (MQ=255)
aaTATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGaaa > 1:3111811/1‑41 (MQ=255)
atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGAt > 1:3099506/1‑58 (MQ=255)
atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGAt > 1:2637789/1‑58 (MQ=255)
atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:3303431/71‑1 (MQ=255)
atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:1767327/71‑1 (MQ=255)
atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:3309076/71‑1 (MQ=255)
atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:2596376/71‑1 (MQ=255)
tatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:2751996/70‑1 (MQ=255)
tatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:1967012/70‑1 (MQ=255)
tGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGaa < 1:4587990/70‑1 (MQ=255)
gTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCaa < 1:3247171/44‑1 (MQ=255)
gTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCaa < 1:3298511/44‑1 (MQ=255)
gTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCaa < 1:1856269/44‑1 (MQ=255)
gTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCaa < 1:2593733/44‑1 (MQ=255)
tCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGc < 1:4322422/71‑1 (MQ=255)
tCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGc < 1:1762859/71‑1 (MQ=255)
tGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCata > 1:4508543/1‑71 (MQ=255)
tGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCg < 1:4196183/42‑1 (MQ=255)
tGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCg < 1:4199082/42‑1 (MQ=255)
gtaggtaTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCtg < 1:4513393/71‑1 (MQ=255)
taggtaTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:3277762/54‑1 (MQ=255)
ggtaTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTgg > 1:135233/1‑71 (MQ=255)
ggtaTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTgg > 1:3695607/1‑71 (MQ=255)
ggtaTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGACATATCACCTGTgg > 1:2704223/1‑71 (MQ=255)
gtaTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGt < 1:2853615/71‑1 (MQ=255)
taTTGGTCAGGTAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATc < 1:3922620/61‑1 (MQ=255)
taTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATc < 1:1275630/61‑1 (MQ=255)
taTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATc < 1:2437388/61‑1 (MQ=255)
ttGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAgg < 1:860678/52‑1 (MQ=255)
ttGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCa > 1:1980668/1‑60 (MQ=255)
ttGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCAc > 1:2414054/1‑61 (MQ=255)
ttGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCAc > 1:3555846/1‑61 (MQ=255)
ttGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCAc > 1:1570136/1‑61 (MQ=255)
ttGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCAt > 1:2218610/1‑71 (MQ=255)
ttGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCAt > 1:2621222/1‑71 (MQ=255)
tGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCt < 1:4638172/43‑1 (MQ=255)
tGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCt < 1:2344619/43‑1 (MQ=255)
tGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCt < 1:1620687/43‑1 (MQ=255)
ggTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCAt > 1:3794158/1‑69 (MQ=255)
gTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTg > 1:1655344/1‑70 (MQ=255)
cAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCg > 1:3954261/1‑71 (MQ=255)
cAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCg > 1:4259778/1‑71 (MQ=255)
cAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCg > 1:3515754/1‑71 (MQ=255)
aGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCt < 1:4714567/38‑1 (MQ=255)
aGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCt < 1:2348375/38‑1 (MQ=255)
aGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCt < 1:4246565/38‑1 (MQ=255)
aGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCg > 1:201027/1‑70 (MQ=255)
aGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGc < 1:3535518/71‑1 (MQ=255)
aGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGc < 1:121590/71‑1 (MQ=255)
aGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGc < 1:108448/71‑1 (MQ=255)
aGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACt > 1:4440097/1‑71 (MQ=255)
aGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACt < 1:3819946/71‑1 (MQ=255)
aaaGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCg < 1:4298120/71‑1 (MQ=255)
aaaGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCg > 1:3363864/1‑71 (MQ=255)
aaGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCg < 1:235979/70‑1 (MQ=255)
aGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCgtg > 1:2353570/1‑71 (MQ=255)
aGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCgtg > 1:4282902/1‑71 (MQ=255)
aGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCgtg > 1:3247909/1‑71 (MQ=255)
aGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCgtg < 1:66885/71‑1 (MQ=255)
aGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCgtg > 1:1758254/1‑71 (MQ=255)
|
CCATCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCGTG > minE/2550290‑2550430
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
