Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,811,028 |
G→A |
54.9% |
A573V (GCA→GTA) |
uvrA ← |
ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,811,028 | 0 | G | A | 54.9%
| 18.7
/ 94.6
| 71 | A573V (GCA→GTA) | uvrA | ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC |
| Reads supporting (aligned to +/- strand): ref base G (25/7); new base A (27/12); total (52/19) |
| Fisher's exact test for biased strand distribution p-value = 4.33e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 |
TGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGC > minE/2810963‑2811089
|
tGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGAc < 1:1840529/70‑1 (MQ=255)
cGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCAc < 1:1049163/70‑1 (MQ=255)
tCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCa > 1:1105284/1‑71 (MQ=255)
tCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCa > 1:3482445/1‑71 (MQ=255)
aTGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCAcc > 1:2684273/1‑71 (MQ=255)
tGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCAccg < 1:2909782/71‑1 (MQ=255)
tGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGAcc < 1:225661/62‑1 (MQ=255)
tACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGc > 1:2651324/1‑56 (MQ=255)
tACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCAccgcc < 1:3296725/71‑1 (MQ=255)
tACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCAccgcc < 1:511513/71‑1 (MQ=255)
aCTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCAccgcc < 1:783463/70‑1 (MQ=255)
cTGCCCGGGCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCAccgccg < 1:1714828/70‑1 (MQ=255)
cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa > 1:3091605/1‑71 (MQ=255)
ccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGaa > 1:408434/1‑70 (MQ=255)
ggTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAacac < 1:167422/71‑1 (MQ=255)
ggTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAacac < 1:1477054/71‑1 (MQ=255)
tCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCt > 1:488330/1‑71 (MQ=255)
tCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCt < 1:1095384/71‑1 (MQ=255)
cAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACtt > 1:1940305/1‑53 (MQ=255)
cAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACtt > 1:2488173/1‑53 (MQ=255)
cAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTg > 1:2098093/1‑71 (MQ=255)
aaaCGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAACAcc > 1:852358/2‑69 (MQ=255)
aaCGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTgc > 1:1061951/1‑71 (MQ=255)
aCGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTgcg > 1:205210/1‑71 (MQ=255)
aCGACTCCGGCACCGCCATAATCGCTTCAAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTgcg > 1:3496016/1‑71 (MQ=255)
cGACTCCGGCACCGCCATAATCGCTTACAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTgcg < 1:2119332/70‑1 (MQ=255)
gACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAac > 1:3070300/1‑62 (MQ=255)
gACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGAc < 1:913364/44‑1 (MQ=255)
gACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGAc < 1:732347/44‑1 (MQ=255)
tCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCg > 1:855989/1‑70 (MQ=255)
tCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCg > 1:3421776/1‑70 (MQ=255)
tCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGAc < 1:93966/41‑1 (MQ=255)
tCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGa > 1:239750/1‑57 (MQ=255)
tCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCgg > 1:3101480/1‑71 (MQ=255)
cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTgc > 1:285407/1‑63 (MQ=255)
cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCgg < 1:2915442/69‑1 (MQ=255)
cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTc > 1:2070475/1‑45 (MQ=255)
cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGc > 1:234621/1‑71 (MQ=255)
ggCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCa > 1:3483597/1‑40 (MQ=255)
gCACCGCCATAATCGCTTCCAGCGGACCTTGTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGcc > 1:3410679/1‑70 (MQ=255)
gCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGcc > 1:3342375/1‑70 (MQ=255)
gCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAac > 1:2639996/1‑55 (MQ=255)
gCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACAGCCGTGAac > 1:3698724/1‑55 (MQ=255)
cGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTc < 1:2039100/39‑1 (MQ=255)
gCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTc > 1:3680066/1‑71 (MQ=255)
ccATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa > 1:2569344/1‑48 (MQ=255)
ccATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa > 1:270076/1‑48 (MQ=255)
ccATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTc > 1:1180594/1‑70 (MQ=255)
aTCGCTTCCAGCGGACCTTCTGCGCCCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATca > 1:322035/1‑70 (MQ=255)
aTCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGcc > 1:1165009/1‑53 (MQ=255)
aTCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATca > 1:3116267/1‑70 (MQ=255)
aTCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATca < 1:2831757/70‑1 (MQ=255)
aTCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATca > 1:3487182/1‑70 (MQ=255)
cGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACAt > 1:3073070/1‑71 (MQ=255)
cGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACAt > 1:185811/1‑71 (MQ=255)
gCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGaa > 1:3524395/1‑40 (MQ=255)
gCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGaa > 1:1687958/1‑40 (MQ=255)
gCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGaa > 1:1691105/1‑40 (MQ=255)
gCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGaa > 1:3160824/1‑40 (MQ=255)
gCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGaa > 1:514976/1‑40 (MQ=255)
gCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATg > 1:2705992/1‑71 (MQ=255)
ccAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGt > 1:1783828/1‑69 (MQ=255)
ccAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGt > 1:2762187/1‑69 (MQ=255)
ccAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGt > 1:1708295/1‑69 (MQ=255)
ccAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGt > 1:3033727/1‑69 (MQ=255)
ggACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAgcggc > 1:1883795/1‑71 (MQ=255)
ggACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAgcgg > 1:1326201/1‑70 (MQ=255)
ggACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAgcgg < 1:2939328/70‑1 (MQ=255)
tCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCg > 1:3526066/1‑48 (MQ=255)
tCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCg > 1:2974053/1‑48 (MQ=255)
tCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAgcgg > 1:1236172/1‑64 (MQ=255)
|
TGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGC > minE/2810963‑2811089
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
