Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F17 I0 R2
|
159 |
90.8 |
4763082 |
92.7% |
4415377 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,811,028 |
G→A |
13.1% |
A573V (GCA→GTA) |
uvrA ← |
ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,811,028 | 0 | G | A | 13.1%
| 210.0
/ 20.4
| 76 | A573V (GCA→GTA) | uvrA | ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC |
| Reads supporting (aligned to +/- strand): ref base G (30/36); new base A (6/4); total (36/40) |
| Fisher's exact test for biased strand distribution p-value = 5.03e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.71e-01 |
GCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAA > minE/2810958‑2811094
|
gCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTg > 1:2821435/1‑71 (MQ=255)
gTTTGCCGCTCATGTACTTCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCg < 1:1671840/71‑1 (MQ=255)
gTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCg < 1:2958654/71‑1 (MQ=255)
gCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGAcc < 1:3164834/70‑1 (MQ=255)
gCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGAcc < 1:2810563/70‑1 (MQ=255)
cGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACt > 1:69885/1‑71 (MQ=255)
gCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACtt < 1:944687/71‑1 (MQ=255)
cTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACtt < 1:700441/70‑1 (MQ=255)
aTGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTc < 1:3133535/68‑1 (MQ=255)
tGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGAc < 1:2249868/61‑1 (MQ=255)
cTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGt < 1:985600/71‑1 (MQ=255)
cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTg < 1:1528356/69‑1 (MQ=255)
cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTg < 1:4376664/69‑1 (MQ=255)
cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTg < 1:3960458/69‑1 (MQ=255)
cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTg < 1:356380/69‑1 (MQ=255)
cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGaa > 1:4706847/1‑71 (MQ=255)
cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGaa > 1:1552059/1‑71 (MQ=255)
cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGaa > 1:2548150/1‑71 (MQ=255)
gTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTg < 1:710204/46‑1 (MQ=255)
gTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTg < 1:3153264/46‑1 (MQ=255)
gTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTg < 1:3143012/46‑1 (MQ=255)
gTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTg < 1:64300/46‑1 (MQ=255)
gTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTg < 1:508406/65‑1 (MQ=255)
gACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGcc > 1:611635/1‑71 (MQ=255)
aCTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCgc > 1:2328244/1‑41 (MQ=255)
aCTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCAc > 1:4052554/1‑46 (MQ=255)
aCTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa > 1:4364087/1‑60 (MQ=255)
aCTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa > 1:4496908/1‑60 (MQ=255)
aCTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa > 1:4146945/1‑60 (MQ=255)
aCTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGccc > 1:4406767/1‑71 (MQ=255)
cTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCg < 1:2342041/71‑1 (MQ=255)
cTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCg < 1:1976424/71‑1 (MQ=255)
cTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCg < 1:3667529/71‑1 (MQ=255)
ccGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGAc < 1:2202489/40‑1 (MQ=255)
cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCa > 1:685526/1‑41 (MQ=255)
cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCTTGAACACCTGCGCCCGGGc > 1:3558788/1‑71 (MQ=255)
cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCggg < 1:1635068/70‑1 (MQ=255)
cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGc > 1:4694610/1‑71 (MQ=255)
cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGc > 1:84760/1‑71 (MQ=255)
cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGc > 1:3666394/1‑71 (MQ=255)
ccGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATg > 1:285668/1‑71 (MQ=255)
cATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGa > 1:1811450/1‑71 (MQ=255)
tAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCaa < 1:381765/63‑1 (MQ=255)
aTCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATca < 1:2273588/70‑1 (MQ=255)
aTCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATca < 1:3930825/70‑1 (MQ=255)
tCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAacac > 1:4510782/1‑45 (MQ=255)
gcgttCCGGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACAt < 1:2095690/68‑1 (MQ=255)
gcgttCCGGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACAt < 1:49177/68‑1 (MQ=255)
gcgttCCGGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACAt < 1:1172885/68‑1 (MQ=255)
gcgttCCGGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACAt < 1:1796270/68‑1 (MQ=255)
gCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa > 1:337159/1‑40 (MQ=255)
gCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa > 1:2544100/1‑40 (MQ=255)
gCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa > 1:2087801/1‑40 (MQ=255)
gCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa > 1:159636/1‑40 (MQ=255)
gCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGa > 1:2294396/1‑39 (MQ=255)
gCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATg < 1:2058151/71‑1 (MQ=255)
gCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATg < 1:3586600/71‑1 (MQ=255)
cTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATgg < 1:1444641/71‑1 (MQ=255)
ccAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCATATGGTc < 1:610894/70‑1 (MQ=255)
ccAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTc < 1:3458192/70‑1 (MQ=255)
ccAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTc < 1:633815/70‑1 (MQ=255)
ccAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTc < 1:2639407/70‑1 (MQ=255)
cAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCa < 1:3724831/70‑1 (MQ=255)
cAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAg < 1:200863/71‑1 (MQ=255)
cAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAg > 1:2714584/1‑71 (MQ=255)
gCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCa < 1:1343331/50‑1 (MQ=255)
cGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTgcg < 1:4066906/40‑1 (MQ=255)
gACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGt > 1:1755507/1‑51 (MQ=255)
gACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAgcggcg < 1:2876388/71‑1 (MQ=255)
aCCTTCTGCGACCACTTCACCGCCGTGAACACCTTCGCCCGGGCCAATGTCGATcac > 1:737291/1‑57 (MQ=255)
aCCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATcac > 1:4045459/1‑57 (MQ=255)
aCCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATcac > 1:4239326/1‑57 (MQ=255)
cTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGcgcg > 1:1821695/1‑70 (MQ=255)
cTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGcgcg > 1:4003757/1‑70 (MQ=255)
cTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGcgcg > 1:2520869/1‑70 (MQ=255)
cTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGcgcg > 1:2515282/1‑70 (MQ=255)
tCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAg > 1:726747/1‑61 (MQ=255)
tCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAg > 1:1728319/1‑61 (MQ=255)
cTGCGACCACTTCACCGCCGTGAACACCTGCGCCCggg > 1:357440/1‑38 (MQ=255)
cTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGaa < 1:3729604/69‑1 (MQ=255)
cTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGaa < 1:618551/69‑1 (MQ=255)
|
GCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAA > minE/2810958‑2811094
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
