Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
983,505 |
T→C |
23.6% |
intergenic (+417/‑151) |
ydfC → / → dicB |
hypothetical protein/cell division inhibition protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 983,505 | 0 | T | C | 23.6%
| 94.7
/ 27.1
| 55 | intergenic (+417/‑151) | ydfC/dicB | hypothetical protein/cell division inhibition protein |
| Reads supporting (aligned to +/- strand): ref base T (24/18); new base C (7/6); total (31/24) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTGACGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCT > minE/983435‑983568
|
gTGACGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGAtgt > 1:3649234/1‑71 (MQ=255)
gACGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATgtgg > 1:1174929/1‑71 (MQ=255)
cGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATgtggtg < 1:3495389/71‑1 (MQ=255)
cGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATgtggtg < 1:1767904/71‑1 (MQ=255)
cGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATgtggt > 1:176120/1‑70 (MQ=255)
tttGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGaa < 1:3659306/71‑1 (MQ=255)
ttGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAAt > 1:4636548/1‑71 (MQ=255)
tGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAAt < 1:4825560/70‑1 (MQ=255)
tGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATg > 1:1136924/1‑71 (MQ=255)
gCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGc < 1:4054426/70‑1 (MQ=255)
tCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAg > 1:3054534/1‑71 (MQ=255)
cAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAgc > 1:1048103/1‑71 (MQ=255)
cAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAgc > 1:3741604/1‑71 (MQ=255)
ttttACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATgtgg < 1:3132195/53‑1 (MQ=255)
ttACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGAtgt > 1:598682/1‑49 (MQ=255)
aCTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCAcgc < 1:3643948/70‑1 (MQ=255)
cGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGaa > 1:3093459/1‑70 (MQ=255)
tGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATgtggtg < 1:974370/45‑1 (MQ=255)
tGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATgtggtg < 1:1066898/45‑1 (MQ=255)
tGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAg > 1:3683709/1‑71 (MQ=255)
aCTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGt > 1:3041768/1‑70 (MQ=255)
tGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCg < 1:4819895/47‑1 (MQ=255)
ctctGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTaaaa > 1:44310/1‑71 (MQ=255)
ctGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAAc > 1:3472261/1‑70 (MQ=255)
tGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGaa > 1:2422739/1‑38 (MQ=255)
tGCCGCCCTTTTTAAAGTGAATTTTGGGATGTGGTGaa > 1:5619713/1‑38 (MQ=255)
ccgccCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTg < 1:3661696/44‑1 (MQ=255)
ccgccCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaa < 1:701072/71‑1 (MQ=255)
gccCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaaa > 1:2859085/1‑71 (MQ=255)
gccCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaaa > 1:3810154/1‑71 (MQ=255)
cccTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaaa > 1:3801619/1‑70 (MQ=255)
cccTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaa < 1:2318810/67‑1 (MQ=255)
cccTTTTTAAAGTGAATTTTGTGATGCGGTGAATGCGGCTAAGCGCAcg > 1:209816/1‑49 (MQ=38)
cccTTTTTAAAGTGAATTTTGTGATGCGGTGAATGCGGCTAAGCGCAcg > 1:5018031/1‑49 (MQ=38)
cccTTTTTAAAGTGAATTTTGTGATGCGGTGAATGCGGCTAAGCGCAcg > 1:4553311/1‑49 (MQ=38)
ccTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAg > 1:2177498/1‑56 (MQ=255)
tttttAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCgg < 1:1431139/49‑1 (MQ=255)
tttttAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCgg < 1:2351362/49‑1 (MQ=255)
tttttAAAGTGAATTTTGTGATGCGGTGAATGCGGCta < 1:1015920/38‑2 (MQ=38)
tttAAAGTGAATTTTGTGATGCGGTGAATGCGGCTAAg > 1:5355539/1‑38 (MQ=37)
tttAAAGTGAATTTTGTGATGCGGTGAATGCGGCTAAGCGCACGTggca > 1:2288969/1‑47 (MQ=37)
ttAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaa < 1:1153227/63‑1 (MQ=255)
ttAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAgtgt < 1:3432727/70‑1 (MQ=255)
ttAAAGAGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAgtgt > 1:60501/1‑70 (MQ=255)
tAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGaa > 1:3522728/1‑47 (MQ=255)
tAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGaa > 1:4790084/1‑47 (MQ=255)
tAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGaa > 1:2777883/1‑47 (MQ=255)
tAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGtt < 1:4752168/70‑1 (MQ=255)
aaaGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAAc > 1:4377844/1‑63 (MQ=255)
aaaGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAAc > 1:5115125/1‑63 (MQ=255)
aaGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTAt < 1:1607623/70‑1 (MQ=255)
tGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCAcg < 1:3577234/37‑1 (MQ=255)
tGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCAcg < 1:4286088/37‑1 (MQ=255)
tGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCAcg < 1:4858309/37‑1 (MQ=255)
gAATTTTGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaaaa > 1:4340373/1‑50 (MQ=255)
gAATTTTGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaaaa > 1:1109611/1‑50 (MQ=255)
aaTTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTg > 1:1791464/1‑70 (MQ=255)
ttttGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaa < 1:1661474/45‑1 (MQ=25)
ttttGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaa < 1:5535740/45‑1 (MQ=25)
ttttGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaa < 1:3726905/45‑1 (MQ=25)
ttGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGAtt > 1:5099483/1‑70 (MQ=255)
gtgATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATtct < 1:3279245/70‑1 (MQ=255)
gATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaa < 1:4210935/39‑1 (MQ=25)
gATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaa < 1:902769/39‑1 (MQ=25)
tggtgAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGtt < 1:1989506/51‑1 (MQ=255)
tggtgAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGtt < 1:4610845/51‑1 (MQ=255)
|
GTGACGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCT > minE/983435‑983568
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
