Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
983,505 |
T→C |
16.3% |
intergenic (+417/‑151) |
ydfC → / → dicB |
hypothetical protein/cell division inhibition protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 983,505 | 0 | T | C | 16.3%
| 94.7
/ 11.6
| 43 | intergenic (+417/‑151) | ydfC/dicB | hypothetical protein/cell division inhibition protein |
| Reads supporting (aligned to +/- strand): ref base T (20/16); new base C (2/5); total (22/21) |
| Fisher's exact test for biased strand distribution p-value = 2.40e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GACGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTAT > minE/983437‑983574
|
gACGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATgtgg > 1:2317026/1‑71 (MQ=255)
tGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAAt < 1:181709/70‑1 (MQ=255)
tGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATg > 1:3943719/1‑71 (MQ=255)
gTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGAtgtg > 1:1075518/1‑59 (MQ=255)
gTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCt < 1:552185/71‑1 (MQ=255)
gTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCt > 1:1225726/1‑71 (MQ=255)
tATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCt > 1:2312566/1‑70 (MQ=255)
cAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAgc > 1:2105614/1‑71 (MQ=255)
gTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGa < 1:3869120/57‑1 (MQ=255)
ttttACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATgtggtg < 1:799446/55‑1 (MQ=255)
tttACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCAc > 1:2003373/1‑71 (MQ=255)
ttACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGaa > 1:1981797/1‑55 (MQ=255)
ttACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCAcg > 1:1446336/1‑71 (MQ=255)
ttACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCAcg > 1:3508298/1‑71 (MQ=255)
ttACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCAc < 1:1242156/70‑1 (MQ=255)
tACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCAcg < 1:1626949/70‑1 (MQ=255)
tCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCgg > 1:703380/1‑70 (MQ=255)
cGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGaa > 1:1744422/1‑70 (MQ=255)
tGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGATTGCGGCTGTGCGCACGCGGAACa < 1:3273197/70‑1 (MQ=255)
cTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGGACGCGGAACAGTTa < 1:1872369/71‑1 (MQ=255)
ctctGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTaaaa > 1:2045059/1‑71 (MQ=255)
ctctGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTaa < 1:1253196/69‑1 (MQ=255)
ctctGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTaa < 1:3112833/69‑1 (MQ=255)
gccgccCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCa > 1:2303951/1‑70 (MQ=255)
cccTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCAcgc < 1:3592838/50‑1 (MQ=255)
ccTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaaa > 1:3708794/1‑69 (MQ=255)
ccTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaaa > 1:2745282/1‑69 (MQ=255)
cTTATTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTaa < 1:2741076/59‑1 (MQ=255)
tttttAAAGTGAATTTTGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaaaa < 1:2502227/60‑1 (MQ=255)
tttttAAAGTGAATTTTGTCATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaaaa < 1:1748936/60‑1 (MQ=255)
ttttAAAGTGAATTTTGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaaaagt < 1:447940/61‑3 (MQ=255)
tttAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGAGCACGCgg > 1:3257778/1‑47 (MQ=255)
tttAAAGTGAATTTTGTGATGCGGTGAATGCGGCTAAGCGCACGTggc > 1:800831/1‑47 (MQ=37)
tAAAGTGAATTTTGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGt < 1:113572/51‑1 (MQ=37)
aaGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTAt < 1:608316/70‑1 (MQ=255)
aaGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTAt < 1:368105/70‑1 (MQ=255)
aaGTGAATTTTGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTa > 1:1951489/1‑51 (MQ=37)
tGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCgg < 1:991038/40‑1 (MQ=255)
tGAATTTTGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAg < 1:2499119/45‑1 (MQ=25)
aaTTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATgg < 1:77106/67‑1 (MQ=255)
aaTTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTg < 1:2978353/70‑1 (MQ=255)
ttttGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGAt > 1:3282629/1‑71 (MQ=255)
ttGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTa > 1:459098/1‑60 (MQ=255)
gtgATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATtctc > 1:1177374/1‑71 (MQ=255)
tgATGTGGTGAATGCGGCTGAGCGCACGCGGAACa > 1:2955667/1‑35 (MQ=255)
gATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTg > 1:1738694/1‑62 (MQ=255)
tgtgGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTa < 1:1642272/71‑1 (MQ=255)
tggtgAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTAt < 1:2617353/70‑1 (MQ=255)
tggtgAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTAt < 1:2612737/70‑1 (MQ=255)
|
GACGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTAT > minE/983437‑983574
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
