Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,191,074 |
A→C |
78.2% |
intergenic (+415/+485) |
yeaJ → / ← rnd |
predicted diguanylate cyclase/ribonuclease D |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,191,074 | 0 | A | C | 78.2%
| 116.6
/ 17.4
| 50 | intergenic (+415/+485) | yeaJ/rnd | predicted diguanylate cyclase/ribonuclease D |
| Reads supporting (aligned to +/- strand): ref base A (5/6); new base C (16/23); total (21/29) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTA > minE/1191010‑1191144
|
tGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAgcgc > 1:5570332/1‑70 (MQ=255)
tGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCt < 1:4673634/71‑1 (MQ=255)
tGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCt < 1:2088420/71‑1 (MQ=255)
tGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCAGGCAATGTCTGCGCTTTTTCCATCAgcgc > 1:1267023/1‑70 (MQ=255)
tCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTag > 1:5608914/1‑71 (MQ=255)
cATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTagc < 1:4215690/70‑1 (MQ=255)
cATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTag > 1:3496956/1‑69 (MQ=255)
cATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTag > 1:5306911/1‑69 (MQ=255)
tCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTagcagc < 1:419628/71‑1 (MQ=255)
tCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTagcagc < 1:5386794/71‑1 (MQ=255)
aGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGt > 1:4852254/1‑71 (MQ=255)
tAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTag > 1:787049/1‑61 (MQ=255)
tAAGCATCGGCTACGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTa > 1:4621321/1‑71 (MQ=255)
aTCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGt > 1:42101/1‑70 (MQ=255)
ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGa > 1:157306/1‑69 (MQ=255)
cGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCg < 1:5407908/69‑1 (MQ=255)
tAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTa < 1:3120347/44‑1 (MQ=255)
tAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTa < 1:1226220/44‑1 (MQ=255)
tAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGAttt > 1:4164948/1‑71 (MQ=255)
tAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGAtt > 1:5564887/1‑70 (MQ=255)
aGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCg > 1:1094284/1‑71 (MQ=255)
aGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCg > 1:3610064/1‑71 (MQ=255)
ggCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGc < 1:2876094/71‑1 (MQ=255)
gCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCt < 1:1975552/71‑1 (MQ=255)
cATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTa < 1:5551022/71‑1 (MQ=255)
aTCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTa < 1:4915245/70‑1 (MQ=255)
aTCTTCCGGCAATGTCTGAGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTAc > 1:2068519/1‑71 (MQ=255)
cTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCg < 1:4176068/58‑1 (MQ=255)
cTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCg < 1:5471537/58‑1 (MQ=255)
cTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTc < 1:573205/64‑1 (MQ=255)
cTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTc < 1:4076684/64‑1 (MQ=255)
cTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCg > 1:1647132/1‑71 (MQ=255)
ttCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTa > 1:3476348/1‑35 (MQ=255)
ccGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTAcc < 1:1848379/67‑1 (MQ=255)
ccGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGa < 1:1000166/70‑1 (MQ=255)
ccGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGa < 1:537302/70‑1 (MQ=255)
cGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATa < 1:553018/71‑1 (MQ=255)
ggCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATaa < 1:865928/71‑1 (MQ=255)
aaTGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTc < 1:1859781/56‑1 (MQ=255)
tGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGAtt < 1:2956484/52‑1 (MQ=255)
tCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAg < 1:4070332/70‑1 (MQ=255)
cgcTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTg < 1:2098382/70‑1 (MQ=255)
gcTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTc > 1:4241315/1‑71 (MQ=255)
tttttCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCgg < 1:1532951/40‑1 (MQ=255)
tttttCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCgg < 1:1113457/40‑1 (MQ=255)
tttttCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCgg < 1:86608/40‑1 (MQ=255)
tttCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGt > 1:1646456/1‑71 (MQ=255)
ttCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAg < 1:3671557/69‑1 (MQ=255)
ttCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGc > 1:1228504/1‑44 (MQ=255)
tCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAAccc > 1:207282/1‑57 (MQ=255)
ccaccaGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTc < 1:665912/70‑1 (MQ=255)
aaGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTa < 1:2564804/71‑1 (MQ=255)
|
TGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTA > minE/1191010‑1191144
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
