Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R2
|
251 |
90.0 |
5200563 |
86.9% |
4519289 |
59.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,191,074 |
A→C |
9.6% |
intergenic (+415/+485) |
yeaJ → / ← rnd |
predicted diguanylate cyclase/ribonuclease D |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,191,074 | 0 | A | C | 9.6%
| 200.6
/ 15.7
| 83 | intergenic (+415/+485) | yeaJ/rnd | predicted diguanylate cyclase/ribonuclease D |
| Reads supporting (aligned to +/- strand): ref base A (46/29); new base C (3/5); total (49/34) |
| Fisher's exact test for biased strand distribution p-value = 2.63e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTA > minE/1191009‑1191144
|
aTGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAgcgc < 1:385864/71‑1 (MQ=255)
tCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTag > 1:2439360/1‑71 (MQ=255)
tCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTag > 1:3836259/1‑71 (MQ=255)
aGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGt < 1:3838548/71‑1 (MQ=255)
aGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGt > 1:3144890/1‑71 (MQ=255)
aGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGt > 1:1128827/1‑71 (MQ=255)
aGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGt > 1:2823985/1‑71 (MQ=255)
aGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGt > 1:1188212/1‑71 (MQ=255)
aGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGt > 1:2374440/1‑71 (MQ=255)
tAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAgcg > 1:4502783/1‑57 (MQ=255)
tAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAgcg > 1:4607299/1‑57 (MQ=255)
aaGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTAc > 1:1468829/1‑71 (MQ=255)
ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACaa > 1:2030400/1‑46 (MQ=255)
ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTa < 1:1466175/52‑1 (MQ=255)
ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTa < 1:3648065/52‑1 (MQ=255)
ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTa < 1:1200429/52‑1 (MQ=255)
ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTa < 1:4803076/52‑1 (MQ=255)
ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTa < 1:2743847/52‑1 (MQ=255)
ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTa < 1:390046/52‑1 (MQ=255)
ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGt > 1:2649070/1‑67 (MQ=255)
ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGa > 1:1627004/1‑69 (MQ=255)
ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGa > 1:3282461/1‑69 (MQ=255)
ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGa > 1:3774901/1‑69 (MQ=255)
ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGa > 1:3705979/1‑69 (MQ=255)
cTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGc < 1:3967781/71‑1 (MQ=255)
cTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGc < 1:3952399/71‑1 (MQ=255)
cTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGc < 1:1277437/71‑1 (MQ=255)
cTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGc < 1:1429474/71‑1 (MQ=255)
cTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGc < 1:3707596/71‑1 (MQ=255)
cTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGc < 1:2613300/71‑1 (MQ=255)
cTGCGGTAAGGCATCATCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGc < 1:2607085/71‑1 (MQ=255)
gTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAg < 1:1159833/65‑1 (MQ=255)
aaGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACaa > 1:2766268/1‑37 (MQ=255)
aaGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACaa > 1:3016226/1‑37 (MQ=255)
aaGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACaa > 1:4752435/1‑37 (MQ=255)
aaGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACaa > 1:4000622/1‑37 (MQ=255)
gCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCt < 1:2908969/71‑1 (MQ=255)
tCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTc > 1:1259917/1‑65 (MQ=255)
tCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTc > 1:2206013/1‑65 (MQ=255)
tCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTc > 1:1266074/1‑65 (MQ=255)
tCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTc > 1:3186529/1‑65 (MQ=255)
tCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTc > 1:1051114/1‑65 (MQ=255)
ttCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTag > 1:5132314/1‑36 (MQ=255)
ttCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCg > 1:981970/1‑70 (MQ=255)
ttCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCg > 1:3257066/1‑70 (MQ=255)
ttCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCg > 1:1102630/1‑70 (MQ=255)
ttCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCg > 1:2718790/1‑70 (MQ=255)
ttCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCg > 1:1458252/1‑70 (MQ=255)
ttCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCg > 1:1254692/1‑70 (MQ=255)
ttCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCg > 1:2336976/1‑70 (MQ=255)
ttCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCg > 1:2415069/1‑70 (MQ=255)
ttCCGG‑AATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGa > 1:4001034/1‑71 (MQ=255)
ccGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGa > 1:4171470/1‑50 (MQ=255)
ccGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGa > 1:2861723/1‑70 (MQ=255)
ccGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGa > 1:268247/1‑70 (MQ=255)
ccGGCAATGTCTGCGCTTTTTCCACAAGCGCTagc > 1:4031149/1‑35 (MQ=255)
ccGGCAATGTCTGCGCTTTTTCCACAAGCGCTagc > 1:1473988/1‑35 (MQ=255)
ccGGCAATGTCTGCGCTTTTTCCACAAGCGCTagc > 1:3364821/1‑35 (MQ=255)
ccGGCAATGTCTGCGCTTTTTCCACAAGCGCTagc > 1:3189356/1‑35 (MQ=255)
cGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATa < 1:589503/71‑1 (MQ=255)
cGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATa < 1:1582787/71‑1 (MQ=255)
ggCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATa > 1:3361730/1‑70 (MQ=255)
cAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAAc > 1:1946850/1‑71 (MQ=255)
cAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAAc > 1:88019/1‑71 (MQ=255)
aaTGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTg < 1:3835397/44‑1 (MQ=255)
aaTGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTg < 1:4981982/44‑1 (MQ=255)
aaTGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTg < 1:3110853/44‑1 (MQ=255)
aaTGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATaaa > 1:1187337/1‑69 (MQ=255)
aaTGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATaaa > 1:3333972/1‑69 (MQ=255)
aaTGTCTGCGCTGTTTCCACAAGCGCTAGCAGCGTTTTACCGTg < 1:3407316/44‑1 (MQ=255)
gTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAg > 1:3171586/1‑71 (MQ=255)
gTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAg > 1:2164819/1‑71 (MQ=255)
tCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGc > 1:4199398/1‑54 (MQ=255)
tCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGc > 1:1611879/1‑54 (MQ=255)
tCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGc > 1:2532299/1‑54 (MQ=255)
cgcTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCt < 1:4176423/69‑1 (MQ=255)
cgcTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCt < 1:1849054/69‑1 (MQ=255)
cgcTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTg < 1:31917/70‑1 (MQ=255)
cgcTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTg < 1:542936/70‑1 (MQ=255)
cgcTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTCAAAGCGGATTTCGCTACCGGATAAACCCAGGCTg < 1:4693089/70‑1 (MQ=255)
cTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCg < 1:2983510/40‑1 (MQ=255)
cTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCg < 1:774652/40‑1 (MQ=255)
cTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCg < 1:739165/40‑1 (MQ=255)
cTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCg < 1:3924470/40‑1 (MQ=255)
cTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCg < 1:1825618/40‑1 (MQ=255)
cTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCg < 1:4205098/40‑1 (MQ=255)
tttCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGt > 1:5066483/1‑71 (MQ=255)
tttCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGt > 1:2343796/1‑71 (MQ=255)
cacaAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCg > 1:4803888/1‑70 (MQ=255)
cacaAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGc > 1:4401485/1‑71 (MQ=255)
aaGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTa < 1:2212229/71‑1 (MQ=255)
|
ATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTA > minE/1191009‑1191144
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
