Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,250,792 |
A→C |
80.4% |
L167L (CTT→CTG) |
ruvC ← |
component of RuvABC resolvasome, endonuclease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,250,792 | 0 | A | C | 80.4%
| 217.1
/ 27.8
| 86 | L167L (CTT→CTG) | ruvC | component of RuvABC resolvasome, endonuclease |
| Reads supporting (aligned to +/- strand): ref base A (11/6); new base C (38/31); total (49/37) |
| Fisher's exact test for biased strand distribution p-value = 5.88e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCG > minE/1250727‑1250859
|
gggagTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGtt > 1:1656491/1‑71 (MQ=255)
ggagTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAg > 1:4034332/1‑67 (MQ=255)
tGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCa < 1:926739/61‑1 (MQ=255)
ggAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCcg > 1:2847345/1‑71 (MQ=255)
gAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTc < 1:2310956/65‑1 (MQ=255)
gAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTc < 1:3858193/65‑1 (MQ=255)
gAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTc < 1:3292639/65‑1 (MQ=255)
gAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTc < 1:5232315/65‑1 (MQ=255)
gAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCcgc > 1:776429/1‑71 (MQ=255)
aaaCGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCcgc < 1:3940179/70‑1 (MQ=255)
aCGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCa < 1:5542831/56‑1 (MQ=255)
gCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTc > 1:2783068/1‑71 (MQ=255)
ccTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCg > 1:3366392/1‑71 (MQ=255)
cTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGc < 1:2606734/71‑1 (MQ=255)
cTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGc > 1:1763296/1‑71 (MQ=255)
cTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGc > 1:1584978/1‑71 (MQ=255)
tCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCa < 1:2921614/51‑1 (MQ=255)
aGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCa > 1:5379213/1‑71 (MQ=255)
aGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCa > 1:1588906/1‑71 (MQ=255)
aGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCa > 1:1139307/1‑56 (MQ=255)
gCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGcc > 1:593392/1‑58 (MQ=255)
gCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCTCTCAt > 1:2521923/1‑71 (MQ=255)
ccGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCAt < 1:2621678/70‑1 (MQ=255)
gAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAgg > 1:3654471/1‑47 (MQ=255)
aCCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCTGCCGCGATTCGCTCATCTGCATCGCa < 1:3995490/71‑1 (MQ=255)
aCCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCg < 1:734720/69‑1 (MQ=255)
aCCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCa < 1:3620735/71‑1 (MQ=255)
aCCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCa < 1:5096119/71‑1 (MQ=255)
aCCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCa > 1:3978096/1‑71 (MQ=255)
aCCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCa < 1:3062776/71‑1 (MQ=255)
aCCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCa < 1:1837034/71‑1 (MQ=255)
aCCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCa > 1:4057136/1‑71 (MQ=255)
ccGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGAtt > 1:5537674/1‑53 (MQ=255)
cGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCAtt > 1:3844930/1‑71 (MQ=255)
aGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGt > 1:3054566/1‑38 (MQ=255)
ggTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGAc > 1:3307743/1‑70 (MQ=255)
ggTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGAc > 1:2327451/1‑70 (MQ=255)
tataACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCcgcg < 1:4703544/41‑1 (MQ=255)
tataACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCcgcg < 1:2878748/41‑1 (MQ=255)
ataACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCAt > 1:3117652/1‑50 (MQ=255)
ataACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCAt > 1:2490159/1‑50 (MQ=255)
ataACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCAt > 1:2192877/1‑50 (MQ=255)
ataACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGa > 1:5252333/1‑66 (MQ=255)
ataACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGa > 1:1742769/1‑66 (MQ=255)
ataACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAAc > 1:4943233/1‑71 (MQ=255)
taACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGcc < 1:4883637/36‑1 (MQ=255)
taACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACg > 1:2442969/1‑71 (MQ=255)
aaCTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGt > 1:2458016/1‑71 (MQ=255)
aaCTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGt > 1:905482/1‑71 (MQ=255)
aaCTTAACACAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGt > 1:3890810/1‑71 (MQ=255)
aCTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGa > 1:477952/1‑38 (MQ=255)
aCTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGAt > 1:5252027/1‑39 (MQ=255)
aCTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCa > 1:2012451/1‑46 (MQ=255)
cTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTgg < 1:2120054/71‑1 (MQ=255)
cTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTgg < 1:3208189/71‑1 (MQ=255)
cTTAACGCAGTCGCCCTCTCGCCAGGTGCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTgg < 1:1684484/71‑1 (MQ=255)
cTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTgg < 1:4063503/71‑1 (MQ=255)
cTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTgg < 1:3337786/71‑1 (MQ=255)
cTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTgg < 1:4846725/71‑1 (MQ=255)
ttAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATc > 1:2089826/1‑46 (MQ=255)
ttAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATc > 1:760888/1‑46 (MQ=255)
ttAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATc > 1:5164677/1‑46 (MQ=255)
aaCGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGc < 1:2961143/69‑1 (MQ=255)
cGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGa > 1:4125567/1‑57 (MQ=255)
gCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTgg > 1:1321803/1‑71 (MQ=255)
cAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACg < 1:854131/61‑1 (MQ=255)
cAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACg < 1:2937428/61‑1 (MQ=255)
cAGTCGCCCTCTCGCCAGGTCCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACg < 1:4306517/61‑1 (MQ=255)
gTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCt < 1:1768167/51‑1 (MQ=255)
gTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTg > 1:1920164/1‑61 (MQ=255)
gTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTg > 1:2940575/1‑61 (MQ=255)
gTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTg > 1:3316480/1‑61 (MQ=255)
gTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGt < 1:493833/66‑1 (MQ=255)
ccTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGa > 1:4146381/1‑48 (MQ=255)
ccTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGa > 1:2300581/1‑48 (MQ=255)
ccTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGa > 1:5046921/1‑48 (MQ=255)
ccTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTACCGTGGCAGTGGGTGATAg > 1:857045/1‑70 (MQ=255)
ccTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAg < 1:2038286/70‑1 (MQ=255)
ctctCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCa < 1:83791/71‑1 (MQ=255)
tctcGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTc < 1:3947265/43‑1 (MQ=255)
tctcGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTc < 1:4308801/43‑1 (MQ=255)
tctcGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCt < 1:879897/44‑1 (MQ=255)
tctcGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTgg > 1:2410675/1‑55 (MQ=255)
tctcGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTg < 1:3427451/64‑1 (MQ=255)
tctcGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTg < 1:3884033/64‑1 (MQ=255)
tctcGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTg < 1:2008479/64‑1 (MQ=255)
tctcGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCaa > 1:676366/1‑71 (MQ=255)
cGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCg > 1:3392074/1‑71 (MQ=255)
gCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCg < 1:1763235/70‑1 (MQ=255)
aaGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGt < 1:3947955/47‑1 (MQ=255)
|
GGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCG > minE/1250727‑1250859
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
