Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,250,792 |
A→C |
54.1% |
L167L (CTT→CTG) |
ruvC ← |
component of RuvABC resolvasome, endonuclease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,250,792 | 0 | A | C | 54.1%
| 42.2
/ 64.1
| 61 | L167L (CTT→CTG) | ruvC | component of RuvABC resolvasome, endonuclease |
| Reads supporting (aligned to +/- strand): ref base A (12/16); new base C (13/20); total (25/36) |
| Fisher's exact test for biased strand distribution p-value = 8.00e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCA > minE/1250725‑1250862
|
gagggagTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAg < 1:132145/70‑1 (MQ=255)
gggagTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGtt < 1:3376865/71‑1 (MQ=255)
ggagTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTc < 1:1267935/71‑1 (MQ=255)
ggagTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTc < 1:1546080/71‑1 (MQ=255)
gagTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCa < 1:3439476/71‑1 (MQ=255)
aaCGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAgg > 1:3366016/1‑60 (MQ=255)
aaCGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGa > 1:897646/1‑71 (MQ=255)
aCGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGAt > 1:704328/1‑71 (MQ=255)
cGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGAtt < 1:3459006/71‑1 (MQ=255)
cTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAg > 1:1404709/1‑54 (MQ=255)
tCAGCCGGAAGTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCt > 1:1593720/1‑71 (MQ=255)
tCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGAtt < 1:2524925/67‑1 (MQ=255)
tCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCt > 1:949991/1‑71 (MQ=255)
aGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCa > 1:303735/1‑71 (MQ=255)
aGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTc < 1:355047/70‑1 (MQ=255)
gCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCAt > 1:284504/1‑71 (MQ=255)
gCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCAt > 1:2718212/1‑71 (MQ=255)
ccGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCa < 1:3232482/69‑1 (MQ=255)
aaCTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCcgc < 1:271920/55‑1 (MQ=255)
aaCTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCa > 1:2751237/1‑71 (MQ=255)
aaCTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCa < 1:1568360/71‑1 (MQ=255)
aaCTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCa > 1:2158845/1‑71 (MQ=255)
aCCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCg < 1:2894932/69‑1 (MQ=255)
aCCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCg < 1:236572/69‑1 (MQ=255)
ccGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCa < 1:577593/70‑1 (MQ=255)
cGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCAtt < 1:50235/71‑1 (MQ=255)
aGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCt > 1:247452/1‑71 (MQ=255)
gCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCa < 1:135771/60‑1 (MQ=255)
gTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGAtt < 1:1624996/45‑1 (MQ=255)
tataACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGAtt < 1:844813/44‑1 (MQ=255)
tataACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCa < 1:304336/56‑1 (MQ=255)
taACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGc < 1:732766/53‑1 (MQ=255)
taACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAAc < 1:2096687/70‑1 (MQ=255)
ttAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTaa < 1:2111905/65‑1 (MQ=255)
ttAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTaa < 1:137700/65‑1 (MQ=255)
ttAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTaa < 1:866174/65‑1 (MQ=255)
ttAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTaa < 1:1497819/65‑1 (MQ=255)
ttAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGt < 1:717332/68‑1 (MQ=255)
tAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTgg > 1:1777447/1‑69 (MQ=255)
tAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTgg > 1:3292912/1‑69 (MQ=255)
aaCGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCt > 1:1141166/1‑57 (MQ=255)
aaCGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACg > 1:1758371/1‑65 (MQ=255)
aaCGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTg > 1:1792170/1‑67 (MQ=255)
aaCGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGc < 1:2696166/69‑1 (MQ=255)
aCGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGt < 1:3719878/71‑1 (MQ=255)
cGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTg > 1:2908209/1‑71 (MQ=255)
cGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTg > 1:3495484/1‑71 (MQ=255)
cGCAGTCGCCCTCTCGCAAGGTTAAGCCGCGATTCGCTCATCTGCATAGCATTCTGACTAACGTGGCAGTg > 1:1143060/1‑71 (MQ=255)
tCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCa < 1:1266173/41‑1 (MQ=255)
tCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCa < 1:1924988/41‑1 (MQ=255)
tCGCCCTCTCGCCAGCTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTTACTAACGTGGCAGTGGGTGa < 1:1116716/71‑1 (MQ=255)
tCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAAc < 1:785167/57‑1 (MQ=255)
tCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGa < 1:1402318/71‑1 (MQ=255)
gCCCTCTCGCCAGGTTCAGCCGCGATTCGCTAATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGa > 1:1953268/1‑69 (MQ=255)
ccTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAg > 1:1670295/1‑70 (MQ=255)
ccTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGc < 1:1095557/71‑1 (MQ=255)
ctctCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGc > 1:479333/1‑70 (MQ=255)
tcGCCAGGTTCAGCCGCGATTTGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCaa < 1:2091331/69‑1 (MQ=255)
tcGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATa > 1:1565399/1‑65 (MQ=255)
gCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATc < 1:2645832/69‑1 (MQ=255)
cAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGc > 1:3544342/1‑70 (MQ=255)
aaGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGc > 1:1712060/1‑69 (MQ=255)
aaGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGc > 1:851149/1‑69 (MQ=255)
aaGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCa < 1:704963/71‑1 (MQ=255)
|
GAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCA > minE/1250725‑1250862
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
