Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,602,585 |
T→C |
22.1% |
intergenic (+121/+119) |
yfeW → / ← yfeX |
predicted periplasmic esterase/conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,602,585 | 0 | T | C | 22.1%
| 241.1
/ 67.4
| 131 | intergenic (+121/+119) | yfeW/yfeX | predicted periplasmic esterase/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (46/56); new base C (18/11); total (64/67) |
| Fisher's exact test for biased strand distribution p-value = 1.41e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.77e-01 |
Only 100 of 138 total aligned reads displayed.
CGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGG > minE/1602515‑1602654
|
gCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGAttt < 1:1279961/71‑1 (MQ=255)
gCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGAttt < 1:2185713/71‑1 (MQ=255)
aaaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCg < 1:2659121/71‑1 (MQ=255)
aaaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCg < 1:3419870/71‑1 (MQ=255)
aaaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCg < 1:1513567/71‑1 (MQ=255)
aaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGAttt > 1:867772/1‑68 (MQ=255)
aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGAtt > 1:1973801/1‑65 (MQ=255)
cTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAgg > 1:5153268/1‑71 (MQ=255)
ccTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGcc < 1:641564/70‑1 (MQ=255)
ccTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCg < 1:2982001/71‑1 (MQ=255)
aTGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGAt < 1:2846181/70‑1 (MQ=255)
aTGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGAt < 1:614292/70‑1 (MQ=255)
aTGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATa < 1:5031299/71‑1 (MQ=255)
tGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATa < 1:1430137/70‑1 (MQ=255)
gtggcaaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGc > 1:3298991‑M1/53‑71 (MQ=255)
tgtcaaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCg > 1:1139451‑M1/52‑71 (MQ=255)
tggcaaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCg > 1:2012125‑M1/52‑71 (MQ=255)
gcaaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCg > 1:2927651‑M1/50‑53 (MQ=255)
gcaaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTa < 1:3622469‑M1/6‑1 (MQ=255)
gcaaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGtt < 1:3459302‑M1/22‑1 (MQ=255)
acaaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATa > 1:2427606‑M1/50‑64 (MQ=255)
aCGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGc > 1:616019/1‑58 (MQ=255)
cGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGc < 1:2024242/57‑1 (MQ=255)
cGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGc < 1:4181072/57‑1 (MQ=255)
cGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGtt < 1:3969903/70‑1 (MQ=255)
aaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCa < 1:4205089‑M1/24‑1 (MQ=255)
aaatgcctgatgcgctacgcttatcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGgcgtcca < 1:4713640‑M1/24‑4 (MQ=255)
cTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGcc > 1:4264186/1‑56 (MQ=255)
cTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCg > 1:3117800/1‑57 (MQ=255)
atgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCTTTCAcg > 1:9523‑M1/46‑71 (MQ=255)
atgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCAc > 1:2198300‑M1/46‑70 (MQ=255)
gcctgatgcgctacgcttatcaggcctacagattctcgcgccaCTCGTAGGCCGGATaa < 1:545159‑M1/16‑1 (MQ=255)
tCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCAcgccg > 1:4105072/1‑71 (MQ=255)
cctgatgcgctacgcttatcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCAcgccg > 1:4313221‑M1/43‑71 (MQ=255)
cctgatgcgctacgcttatcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCAcgccg > 1:5410137‑M1/43‑71 (MQ=255)
cAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCAcgccg > 1:3341650/1‑70 (MQ=255)
cAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCAcgccg > 1:4164196/1‑70 (MQ=255)
tgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTa < 1:921575‑M1/6‑1 (MQ=255)
ctgatgcgctacgcttatcaggcctacaattctcgcgccaCTCGTAGGCCGGATAAGGCGTTCACGCCGCa > 1:4208134‑M1/41‑71 (MQ=255)
aGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCAcgccgc < 1:5149331/70‑1 (MQ=255)
gatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCAt < 1:3601042‑M1/32‑1 (MQ=255)
gatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCAt < 1:3279022‑M1/32‑1 (MQ=255)
atgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCATc > 1:1533701‑M1/39‑71 (MQ=255)
atgcgctacgcttatcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCACGCCGCa > 1:884991‑M1/39‑69 (MQ=255)
atgcgctacgcttatcaggcctacaattctcgcgccaCTCGTa < 1:2265395‑M1/6‑1 (MQ=255)
gcgctacgcttatcaggcctacagattcttgcgccaTTCGTa < 1:3864489‑M1/6‑1 (MQ=255)
gcgctacgcttatcaggcctacagattcttgcgccaTTCGTa < 1:1525568‑M1/6‑1 (MQ=255)
gcgctacgcttatcaggcctacagattcttgcgccaTTCGTa < 1:2108340‑M1/6‑1 (MQ=255)
gcgctacgcttatcaggcctacagattcttgcgccaTTCGTa < 1:535221‑M1/6‑1 (MQ=255)
gcgctacgcttatcaggcctacagattctcgcgccaCTCg > 1:1097183‑M1/37‑40 (MQ=255)
gcgctacgcttatcaggcctacagattctcgcgccaCTCg > 1:4861843‑M1/37‑40 (MQ=255)
gcgctacgcttatcaggcctacagattctcgcgccaCTCg > 1:1371043‑M1/37‑40 (MQ=255)
gcgctacgcttatcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCg < 1:534969‑M1/35‑1 (MQ=255)
tACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGtt < 1:2807604/57‑1 (MQ=255)
tACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg > 1:4429880/1‑71 (MQ=255)
cgctacgcttatcaggcctacaattctcgcgccaCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg < 1:3979161‑M1/36‑1 (MQ=255)
gctacgcttatcaggcctacaattctcgcgccaCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc > 1:5620472‑M1/34‑70 (MQ=255)
ctacgcttatcaggcctacagattcttgcgccaTTc > 1:2081101‑M1/34‑36 (MQ=255)
ctacgcttatcaggcctacagattcttgcgccaTTc > 1:2413192‑M1/34‑36 (MQ=255)
cGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCa > 1:2535100/1‑57 (MQ=255)
cGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg > 1:4481191/1‑69 (MQ=255)
tacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCg > 1:5606946‑M1/33‑43 (MQ=255)
tGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCgtg < 1:536118/71‑1 (MQ=255)
cgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGgca < 1:73329‑M1/38‑2 (MQ=255)
gAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCgtgt > 1:2940791/1‑70 (MQ=255)
tatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAcat < 1:768055‑M1/45‑2 (MQ=255)
tatcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCACGCCGCATcc > 1:1181507‑M1/27‑60 (MQ=255)
tatcaggcctacaaattcccgcgccaTCCGTAGGCCGGATAAGGCGTTCAcgccgc > 1:2548375‑M1/27‑56 (MQ=255)
atcaggcctacagattcttgcgccaTTCGTAGGCCgg < 1:4443203‑M1/12‑1 (MQ=255)
tcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAacatg > 1:769987‑M1/25‑68 (MQ=255)
tcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCg < 1:4204713‑M1/35‑1 (MQ=255)
tcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCg < 1:2144493‑M1/35‑1 (MQ=255)
tcaggcctacaaattcccgcaccaTCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc > 1:4522534‑M1/25‑61 (MQ=255)
cacaATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCg < 1:2953306/44‑1 (MQ=255)
cacaATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCg < 1:3730246/44‑1 (MQ=255)
cacaATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCg < 1:5560262/44‑1 (MQ=255)
caATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCg < 1:4705983/42‑1 (MQ=38)
caATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCg < 1:2927814/42‑1 (MQ=38)
caATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGtt > 1:5003444/1‑44 (MQ=38)
caATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGtt > 1:1857770/1‑44 (MQ=38)
caATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGtt > 1:841473/1‑44 (MQ=38)
atatatTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTc > 1:2608166/1‑43 (MQ=255)
tatatTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTc > 1:2734752/1‑42 (MQ=255)
tatatTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCAc > 1:5068593/1‑71 (MQ=255)
tatTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCa > 1:1222205/1‑41 (MQ=255)
tatTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCa < 1:1007690/48‑1 (MQ=255)
tacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCACGCCGCATc > 1:2597924‑M1/18‑50 (MQ=255)
atTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCAcgc < 1:195599/43‑1 (MQ=255)
ttGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg > 1:801883/1‑50 (MQ=255)
ttGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg > 1:280150/1‑50 (MQ=255)
cagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCa < 1:3717949‑M1/24‑1 (MQ=255)
cagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCa < 1:4790849‑M1/31‑1 (MQ=255)
cagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCa < 1:315120‑M1/31‑1 (MQ=255)
cagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCa < 1:3579973‑M1/31‑1 (MQ=255)
gattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCAcgccg < 1:870899‑M1/29‑1 (MQ=255)
gAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCa > 1:1994417/1‑37 (MQ=255)
ttctcacgccaTCCGTAGGCCGGATAAGGCGTTCAcgcc < 1:2525628‑M1/28‑1 (MQ=255)
ttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCa < 1:2060699‑M1/31‑1 (MQ=255)
aCGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCt > 1:2755046/1‑71 (MQ=255)
tttCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAgg < 1:2886075/71‑1 (MQ=255)
|
CGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGG > minE/1602515‑1602654
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
