Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,607,958 |
G→A |
G49S (GGT→AGT) |
lsrF → |
predicted aldolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,607,958 | 0 | G | A | 100.0%
| 76.9
/ NA
| 24 | G49S (GGT→AGT) | lsrF | predicted aldolase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (8/16); total (8/16) |
TTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGG > W3110S.gb/1607912‑1608013
|
ttGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGAGTAAAACCGTGATg < 1:1617401/61‑1 (MQ=255)
ttGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGAGTAAAACCGTGATg < 1:2405914/61‑1 (MQ=255)
ttGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGAGTAAAACCGTGATg < 1:2557993/61‑1 (MQ=255)
ttGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGAGTAAAACCGTGATg < 1:416586/61‑1 (MQ=255)
ttGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGAGTAAAACCGTGATg < 1:3073246/61‑1 (MQ=255)
ttGGGGAATGCAGTCACCCTTATCGCGGATATTTAATCCGAAAACGAGTAAAACCGTGATg < 1:1353622/61‑1 (MQ=255)
tCGCGGATATTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGtt > 1:1783405/1‑58 (MQ=255)
gcgGATATTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTc < 1:2565459/62‑1 (MQ=255)
gcgGATATTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTc < 1:3941635/62‑1 (MQ=255)
ggATATTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCa > 1:4693767/1‑61 (MQ=255)
ggATATTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCa > 1:461506/1‑61 (MQ=255)
tatTTAATCCGAAAACGAGTAAAACCGTGATTCTGGCTTTTGACCATGGTTATTTTCAGGGa < 1:2965539/62‑1 (MQ=255)
tatTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGa < 1:915298/62‑1 (MQ=255)
tatTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGa < 1:1014130/62‑1 (MQ=255)
tatTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGa < 1:53982/62‑1 (MQ=255)
tatTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGa < 1:3432276/62‑1 (MQ=255)
tatTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGa < 1:1528080/62‑1 (MQ=255)
tatTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGa < 1:1173428/62‑1 (MQ=255)
atTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGa < 1:3139066/61‑1 (MQ=255)
cGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTAc > 1:290964/1‑61 (MQ=255)
cGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTAc > 1:4480099/1‑61 (MQ=255)
cGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTAc > 1:2517771/1‑61 (MQ=255)
aaaaCGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGa > 1:2328033/1‑51 (MQ=255)
aaaaCGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCgg > 1:4575829/1‑62 (MQ=255)
|
TTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGG > W3110S.gb/1607912‑1608013
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
