Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R1
|
826 |
0.0 |
88706 |
74.7% |
66263 |
57.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
302,241 |
G→A |
P9P (CCG→CCA) |
yagU → |
conserved inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 302,241 | 0 | G | A | 97.1%
| 57.6
/ ‑6.7
| 34 | P9P (CCG→CCA) | yagU | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (0/33); minor base T (0/1); total (0/34) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TAGATATGAATATATTTGAACAAACTCCACCGAACCGCA > W3110S.gb/302210‑302248
|
tAGATATGAATATATTTGAACAAACTCCACCTAACCGCa < 1:74186/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:69910/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:16411/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:49310/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:51558/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:54894/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:56345/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:6136/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:68389/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:69341/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:49051/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:73204/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:76184/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:80504/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:83873/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:84252/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:84345/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:27311/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:16504/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:16513/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:17586/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:231/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:25825/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:26898/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:43809/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:30598/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:32355/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:33428/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:34843/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:38443/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:38886/39‑1 (MQ=255)
tAGATATGAATATATTTGAACAAACTCAACCAAACCGCa < 1:23537/39‑1 (MQ=37)
aGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:59378/38‑1 (MQ=255)
aGATATGAATATATTTGAACAAACTCCACCAAACCGCa < 1:20706/38‑1 (MQ=255)
|
TAGATATGAATATATTTGAACAAACTCCACCGAACCGCA > W3110S.gb/302210‑302248
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
