Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 826 0.0 88706 74.7% 66263 57.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,442,292 G→A G69G (GGC→GGT)  ydbK ← fused predicted Fe‑S subunit of pyruvate‑flavodoxin oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,442,2920GA100.0% 142.7 / NA 79G69G (GGC→GGT) ydbKfused predicted Fe‑S subunit of pyruvate‑flavodoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (79/0);  total (79/0)

GCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGT  >  W3110S.gb/1442285‑1442343
       |                                                   
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGCCTGCATTTCAACCAcgcg   >  1:11115/1‑58 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTg                  >  1:53375/1‑43 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCAcgcgc  >  1:23583/1‑58 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCAcgcg   >  1:60188/1‑58 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCAcgcg   >  1:18163/1‑58 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:65213/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:7072/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:64009/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:63937/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:63936/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:63912/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:63841/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:6327/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:62960/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:62493/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:6045/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:58598/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:55916/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:55704/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:5570/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:55008/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:54452/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:77293/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:87612/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:87124/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:83414/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:82136/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:81127/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:79754/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:78476/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:77668/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:77476/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:68354/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:75622/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:75508/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:7547/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:74997/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:74587/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:71552/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:5129/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:6865/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:20957/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:37901/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:37274/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:35929/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:34905/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:33074/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:32206/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:31965/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:3004/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:22921/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:38168/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:16930/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:16910/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:13528/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:12846/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:11829/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:1164/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:11304/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:10945/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:45053/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:51641/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:10727/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:4932/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:49206/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:47302/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:46221/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:46145/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:45828/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:52539/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:4502/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:41837/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:40219/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:40080/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:4/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:39996/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:39963/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:39858/1‑59 (MQ=255)
gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACAACGCgt  >  1:50808/1‑59 (MQ=255)
       |                                                   
GCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGT  >  W3110S.gb/1442285‑1442343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A