Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R1
|
826 |
0.0 |
88706 |
74.7% |
66263 |
57.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,997,113 |
G→A |
D128D (GAC→GAT) |
uvrY ← |
DNA‑binding response regulator in two‑component regulatory system with BarA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,997,113 | 0 | G | A | 100.0%
| 26.3
/ NA
| 17 | D128D (GAC→GAT) | uvrY | DNA‑binding response regulator in two‑component regulatory system with BarA |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (0/17); total (0/17) |
TGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGA > W3110S.gb/1997077‑1997118
|
tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa < 1:64754/42‑1 (MQ=255)
tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa < 1:88229/42‑1 (MQ=255)
tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa < 1:86387/42‑1 (MQ=255)
tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa < 1:83428/42‑1 (MQ=255)
tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa < 1:75514/42‑1 (MQ=255)
tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa < 1:71746/42‑1 (MQ=255)
tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa < 1:71695/42‑1 (MQ=255)
tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa < 1:71265/42‑1 (MQ=255)
tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa < 1:66486/42‑1 (MQ=255)
tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa < 1:19968/42‑1 (MQ=255)
tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa < 1:55837/42‑1 (MQ=255)
tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa < 1:54179/42‑1 (MQ=255)
tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa < 1:49462/42‑1 (MQ=255)
tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa < 1:23592/42‑1 (MQ=255)
tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa < 1:22007/42‑1 (MQ=255)
tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa < 1:20845/42‑1 (MQ=255)
tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa < 1:20042/42‑1 (MQ=255)
|
TGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGA > W3110S.gb/1997077‑1997118
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
