Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 826 0.0 88706 74.7% 66263 57.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,844,165 C→T A321A (GCG→GCA)  hycE ← hydrogenase 3, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,844,1650CT100.0% 143.2 / NA 80A321A (GCG→GCA) hycEhydrogenase 3, large subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/80);  total (0/80)

GGTTTTACGCGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGGAAGA  >  W3110S.gb/2844156‑2844217
         |                                                    
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATTGAGGTTTCACGCACGCGgaaga  <  1:4519/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:5215/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:62336/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:62123/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:61868/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:60275/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:59495/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:58860/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:58143/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:55497/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:52734/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:63352/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:51864/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:51748/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:50907/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:50813/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:50647/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:49986/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:4934/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:46789/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:76322/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:9127/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:85647/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:84111/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:79330/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:7918/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:78085/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:77947/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:77843/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:76747/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:46681/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:74803/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:72284/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:72096/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:71509/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:71459/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:70826/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:7015/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:69400/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:63572/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:19158/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:10644/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:25979/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:25744/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:25403/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:24464/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:23397/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:22337/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:20962/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:19248/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:26109/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:18979/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:18943/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:18397/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:18371/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:17764/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:17047/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:15489/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:15290/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:11300/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:39801/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:46626/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:46435/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:46398/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:44023/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:4332/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:43073/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:41598/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:40927/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:26664/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:39476/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:39421/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:39411/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:36099/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:35617/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:33498/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:29495/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:28201/62‑1 (MQ=255)
ggTTTTACGTGCACCGGTAAGGATCTCTGCCATCTTCATGGAGGTTTCACGCACGCGgaaga  <  1:79235/62‑1 (MQ=255)
    ttACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:28831/58‑1 (MQ=255)
         |                                                    
GGTTTTACGCGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGGAAGA  >  W3110S.gb/2844156‑2844217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A