Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R1
|
826 |
0.0 |
88706 |
74.7% |
66263 |
57.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,625,182 |
2 bp→AT |
coding (423‑424/900 nt) |
yigM ← |
predicted inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,625,182 | 0 | G | A | 100.0%
| 74.2
/ NA
| 42 | L142L (CTG→TTG) | yigM | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (0/42); total (0/42) |
| * | W3110S.gb | 3,625,183 | 0 | C | T | 100.0%
| 71.1
/ NA
| 42 | L141L (TTG→TTA) | yigM | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (0/42); total (0/42) |
TGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATG > W3110S.gb/3625164‑3625200
||
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:74139/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:1020/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:39935/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:49309/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:49432/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:50642/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:5207/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:69145/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:69510/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:69576/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:72314/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:35992/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:74359/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:75696/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:75839/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:76492/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:78778/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:78862/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:83583/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:86654/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:8782/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:31216/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:13129/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:14424/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:15003/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:15402/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:16748/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:18671/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:1966/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:19667/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:22307/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:23113/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:23434/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:26025/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:26699/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:30421/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:30618/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:3353/37‑1 (MQ=25)
tGGAGAGTTGCACCAGCAATAAGCAAGTCCAGAAATg < 1:1670/37‑1 (MQ=21)
ggAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:15534/36‑1 (MQ=25)
ggAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:46241/36‑1 (MQ=25)
ggAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATg < 1:10787/36‑1 (MQ=25)
||
TGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATG > W3110S.gb/3625164‑3625200
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
