Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 826 0.0 88706 74.7% 66263 57.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,867,165 A→T A324A (GCT→GCA)  mtlA ← fused mannitol‑specific PTS enzyme IIABC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,867,1650AT100.0% 163.6 / NA 90A324A (GCT→GCA) mtlAfused mannitol‑specific PTS enzyme IIABC components
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (0/90);  total (0/90)

AGCAGAGACAACGAAGGAGACAGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAG  >  W3110S.gb/3867144‑3867205
                     |                                        
tgCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:65013/61‑1 (MQ=255)
ggCATAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:64087/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGGACACACACCCGCGATGTTAGCGAAGTAAg  <  1:59249/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:56303/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:65134/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:64498/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:62359/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:58857/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:58010/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:57088/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:56320/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:65759/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:56250/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:55944/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:55924/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:5521/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:54583/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:53026/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:52342/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:51753/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:50135/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:11027/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:77222/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:8829/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:87514/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:85892/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:85879/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:85285/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:8255/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:81308/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:78913/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:78447/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:78299/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:49060/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:74208/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:73342/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:73045/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:71649/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:71622/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:70804/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:69898/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:69759/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:67174/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:66147/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:18754/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:48339/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:28449/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:27445/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:25812/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:25335/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:23988/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:23618/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:23419/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:19330/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:18824/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:29598/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:18040/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:16805/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:15841/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:15817/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:15668/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:15258/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:14943/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:14579/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:14188/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:11488/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:40548/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:47920/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:47835/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:44824/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:44744/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:43680/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:42722/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:42302/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:40979/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:31123/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:3908/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:39019/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:38993/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:38673/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:38486/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:36929/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:36318/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:3474/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:3360/61‑1 (MQ=255)
ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGAGAAGTAAg  <  1:8708/61‑1 (MQ=255)
 gCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:33348/61‑1 (MQ=255)
      gaCAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:51895/56‑1 (MQ=255)
             aaGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:35942/49‑1 (MQ=255)
               ggAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:15474/47‑1 (MQ=255)
                     |                                        
AGCAGAGACAACGAAGGAGACAGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAG  >  W3110S.gb/3867144‑3867205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A