Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R1
|
826 |
0.0 |
88706 |
74.7% |
66263 |
57.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,343,430 |
+C |
coding (1775/2271 nt) |
adiA ← |
biodegradative arginine decarboxylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,343,426 | 1 | . | C | 100.0%
| 13.5
/ NA
| 10 | G593G (GGA→GGG) | adiA | biodegradative arginine decarboxylase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (10/0); total (10/0) |
CAAAGGGTGTTAACCAGAGTT‑CCCCATTTCCCACGGGTTACGCCCATAGAGAACAGGAAC > W3110S.gb/4343406‑4343465
|
cAAAGGGTGTTAACCAGAGTTCCCCCATTTCCCACGGGTTACGccc > 1:56662/1‑46 (MQ=38)
cAAAGGGTGTTAACCAGAGTTCCCCCATTTCCCACGGGTTACGCCCATAGAGAACAGGaa > 1:30746/1‑60 (MQ=255)
cAAAGGGTGTTAACCAGAGTTCCCCCATTTCCCACGGGTTACGCCCATAGAGAACAGGAAc > 1:47348/1‑61 (MQ=255)
cAAAGGGTGTTAACCAGAGTTCCCCCATTTCCCACGGGTTACGCCCATAGAGAACAGGAAc > 1:54051/1‑61 (MQ=255)
cAAAGGGTGTTAACCAGAGTTCCCCCATTTCCCACGGGTTACGCCCATAGAGAACAGGAAc > 1:73404/1‑61 (MQ=255)
cAAAGGGTGTTAACCAGAGTTCCCCCATTTCCCACGGGTTACGCCCATAGAGAACAGGAAc > 1:74590/1‑61 (MQ=255)
cAAAGGGTGTTAACCAGAGTTCCCCCATTTCCCACGGGTTACGCCCATAGAGAACAGGAAc > 1:80801/1‑61 (MQ=255)
cAAAGGGTGTTAACCAGAGTTCCCCCATTTCCCACGGGTTACGCCCATAGAGAACAGGAAc > 1:81913/1‑61 (MQ=255)
cAAAGGGTGTTAACCAGAGTTCCCCCATTTCCCACGGGTTACGCCCATAGAGAACAGGAAc > 1:83315/1‑61 (MQ=255)
cAAAGGGTGTTAACCAGAGTTCCCCCATTTCCCACGGGTTACGCCCATAGAGAACAGGAAc > 1:8941/1‑61 (MQ=255)
|
CAAAGGGTGTTAACCAGAGTT‑CCCCATTTCCCACGGGTTACGCCCATAGAGAACAGGAAC > W3110S.gb/4343406‑4343465
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
