Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,030,578 |
2 bp→AC |
coding (93‑94/279 nt) |
yccX → |
predicted acylphosphatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,030,578 | 0 | T | A | 86.1%
| 116.1
/ 5.2
| 43 | G31G (GGT→GGA) | yccX | predicted acylphosphatase |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base A (0/37); total (0/43) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| * | W3110S.gb | 1,030,579 | 0 | T | C | 86.1%
| 103.5
/ 7.9
| 43 | L32L (TTA→CTA) | yccX | predicted acylphosphatase |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base C (0/37); total (0/43) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCG > W3110S.gb/1030537‑1030633
||
ggATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCaaaaa < 1:1037539/62‑1 (MQ=255)
ggATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCaaaaa < 1:2204525/62‑1 (MQ=255)
ggATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCaaaaa < 1:2830012/62‑1 (MQ=255)
ggATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCaaaaa < 1:316242/62‑1 (MQ=255)
ggATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCaaaaa < 1:384998/62‑1 (MQ=255)
ggATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCaaaaa < 1:812608/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:2813776/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:2376187/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:2453148/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:875584/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:2457185/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:2480185/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:2534812/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:2543124/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:2578003/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:751393/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:1003779/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:297411/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:84603/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:468806/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:542204/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:659056/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:725780/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:2343724/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:1152937/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:1159283/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:1363348/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:1473703/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:1517720/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:1552107/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:1585650/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:1905620/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:2016205/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:2053923/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:2130164/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:2133533/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:2135281/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:2152235/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:2161739/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:2362141/62‑1 (MQ=255)
aCTGGGACTAACCGGGTACACCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:167299/62‑1 (MQ=255)
aCTGGGACTAACCGGGGACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:185429/62‑1 (MQ=255)
cTGGGACTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCCTGCg < 1:1814548/61‑1 (MQ=255)
||
GGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCG > W3110S.gb/1030537‑1030633
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
