Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,757,552 |
T→G |
I47M (ATT→ATG) |
pykF → |
pyruvate kinase I |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,757,552 | 0 | T | G | 100.0%
| 181.4
/ NA
| 53 | I47M (ATT→ATG) | pykF | pyruvate kinase I |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (5/48); total (5/48) |
TATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAA > W3110S.gb/1757501‑1757579
|
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:287241/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:2130083/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:2212687/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:225210/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:2498112/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:2514842/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:2539927/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:2549509/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:2550561/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:2581834/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:2643200/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:2734479/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:2216678/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:518361/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:55284/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:633935/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:649179/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:687608/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:707486/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:883711/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:899908/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:930853/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:955934/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:986601/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:1577544/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:1220213/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:1317720/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:1365476/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:1393391/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:1399396/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:1451682/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:1511481/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:1538906/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:1562693/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:1569327/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:1574266/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:2112723/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:1689398/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:1726626/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:1750953/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:1765866/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:1768394/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:1777437/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:1873765/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:1928005/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:2053359/62‑1 (MQ=255)
tATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:2056591/62‑1 (MQ=255)
aTGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATGCAGAATCTgc < 1:9617/61‑1 (MQ=255)
ttcAGAACACGGTCAGCGCATGCAGAATCTGCGCAACGTGATGAGCaaa > 1:1129688/3‑49 (MQ=255)
tGCAGAACACGGTCAGCGCATGCAGAATCTGCGCAACGTGATGAGCaaa > 1:2650819/1‑49 (MQ=255)
tGCAGAACACGGTCAGCGCATGCAGAATCTGCGCAACGTGATGAGCaaa > 1:72005/1‑49 (MQ=255)
tGCAGAACACGGTCAGCGCATGCAGAATCTGCGCAACGTGATGAGCaaa > 1:2299932/1‑49 (MQ=255)
tGCAGAACACGGTCAGCGCATGCAGAATCTGCGCAACGTGATGAGCaaa > 1:906926/1‑49 (MQ=255)
|
TATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAA > W3110S.gb/1757501‑1757579
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
