Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,490,667 |
T→C |
F283S (TTT→TCT) |
evgS → |
hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,490,667 | 0 | T | C | 100.0%
| 21.3
/ NA
| 8 | F283S (TTT→TCT) | evgS | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (8/0); total (8/0) |
GCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAAC > W3110S.gb/2490644‑2490705
|
gCTTGATACAGGAAACCTGGCCTCTCTGAACAAACCATTAGAACTCACTGAACATGaaaaa > 1:1576732/1‑61 (MQ=255)
gCTTGATACAGGAAACCTGGCCTCTCTGAACAAACCATTAGAACTCACTGAACATGAAAAAc > 1:1825616/1‑62 (MQ=255)
gCTTGATACAGGAAACCTGGCCTCTCTGAACAAACCATTAGAACTCACTGAACATGAAAAAc > 1:2064348/1‑62 (MQ=255)
gCTTGATACAGGAAACCTGGCCTCTCTGAACAAACCATTAGAACTCACTGAACATGAAAAAc > 1:2321007/1‑62 (MQ=255)
gCTTGATACAGGAAACCTGGCCTCTCTGAACAAACCATTAGAACTCACTGAACATGAAAAAc > 1:2577038/1‑62 (MQ=255)
gCTTGATACAGGAAACCTGGCCTCTCTGAACAAACCATTAGAACTCACTGAACATGAAAAAc > 1:2635018/1‑62 (MQ=255)
gCTTGATACAGGAAACCTGGCCTCTCTGAACAAACCATTAGAACTCACTGAACATGAAAAAc > 1:606803/1‑62 (MQ=255)
gCTTGATACAGGAAACCTGGCCTCTCTGAACAAAACATTAGAACTCACTGAACATGAAAAAc > 1:686193/1‑62 (MQ=255)
|
GCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAAC > W3110S.gb/2490644‑2490705
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
