Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,971,622 |
A→G |
F299F (TTT→TTC) |
ygeD ← |
predicted inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,971,622 | 0 | A | G | 99.1%
| 355.9
/ ‑4.9
| 105 | F299F (TTT→TTC) | ygeD | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (104/0); total (105/0) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 |
Only 100 of 163 total aligned reads displayed.
CCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCA > W3110S.gb/2971563‑2971662
|
cccATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa < 1:2239184/62‑1 (MQ=255)
cccATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa < 1:1116619/62‑1 (MQ=255)
cccATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa < 1:424770/62‑1 (MQ=255)
cccATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa < 1:74819/62‑1 (MQ=255)
cccATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa < 1:631266/62‑1 (MQ=255)
cATTACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaaaa < 1:1603474/62‑1 (MQ=255)
cATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaaaa < 1:488011/62‑1 (MQ=255)
cATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaaaa < 1:2757818/62‑1 (MQ=255)
cATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaaaa < 1:2146771/62‑1 (MQ=255)
cATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGAAGCTCGTGTTGCAGGGaaaaaa < 1:1120927/62‑1 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:305707/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:1864173/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:1963639/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:178145/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:21719/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:1757968/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:2209183/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:1724240/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:2252709/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:2323133/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:2586659/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:96723/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:314813/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:359883/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:443774/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:597664/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:839956/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:922881/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:1007972/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:1093465/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:1667670/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:1303260/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:1133932/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:1156125/1‑49 (MQ=255)
tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:1332340/1‑49 (MQ=255)
gCATCAACAAAGCATAGGCTGGCCGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2909360/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATc > 1:2740355/1‑50 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2732259/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2919176/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2906844/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2887865/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2797036/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:274501/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1287996/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2651624/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2647026/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2621838/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1352769/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1692578/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:296463/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1323819/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2579065/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:375815/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1269867/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1262895/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1238738/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:494613/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:502325/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:539403/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:546162/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1224383/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:789816/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:804431/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1030023/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:214312/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1697152/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1668101/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1762666/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1621264/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:159572/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:187563/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:190575/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1931860/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1961400/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1589289/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2032268/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2068313/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2073996/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2532691/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1554654/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:233646/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2527058/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:247932/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:241119/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2405612/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2370818/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2343810/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1543528/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1423526/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2269736/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2258779/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:146338/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:15002/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:153230/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2195448/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacc > 1:471771/1‑56 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTAccaccaag > 1:1871627/1‑60 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTAccaccaag > 1:924669/1‑60 (MQ=255)
gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTACAGGGAGAAAATCAGTacca > 1:2326583/1‑57 (MQ=255)
gCATCAACAAAGCATAGGCTCGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1071658/1‑57 (MQ=255)
|
CCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCA > W3110S.gb/2971563‑2971662
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
