Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,551,414 |
A→G |
M186V (ATG→GTG) |
fdoG → |
formate dehydrogenase‑O, large subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,551,414 | 0 | A | G | 100.0%
| 18.1
/ NA
| 7 | M186V (ATG→GTG) | fdoG | formate dehydrogenase‑O, large subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (0/7); total (0/7) |
GGCTATTTAACGCAAAAATTCTCCCGCGCGCTGGGTATGCTCGCGGTCGACAACCAGGCGCG > W3110S.gb/3551378‑3551439
|
ggCTATTTAACGCAAAAATTCTCCCGCGCGCTGGGTGTGCTCGCGGTCGACAACCAGGcgcg < 1:1054345/62‑1 (MQ=255)
ggCTATTTAACGCAAAAATTCTCCCGCGCGCTGGGTGTGCTCGCGGTCGACAACCAGGcgcg < 1:1521023/62‑1 (MQ=255)
ggCTATTTAACGCAAAAATTCTCCCGCGCGCTGGGTGTGCTCGCGGTCGACAACCAGGcgcg < 1:2726229/62‑1 (MQ=255)
ggCTATTTAACGCAAAAATTCTCCCGCGCGCTGGGTGTGCTCGCGGTCGACAACCAGGcgcg < 1:595210/62‑1 (MQ=255)
ggCTATTTAACGCAAAAATTCTCCCGCGCGCTGGGTGTGCTCGCGGTCGACAACCAGGcgcg < 1:667936/62‑1 (MQ=255)
ggCTATTTAACGCAAAAATTCTCCCGCGCGCTGGGTGTGCTCGCGGTCGACAACCAGGcgcg < 1:885467/62‑1 (MQ=255)
gCTATTTAACGCAAAAATTCTCCCGCGCGCTGGGTGTGCTCGCGGTCGACAACCAGGcgcg < 1:1435492/61‑1 (MQ=255)
|
GGCTATTTAACGCAAAAATTCTCCCGCGCGCTGGGTATGCTCGCGGTCGACAACCAGGCGCG > W3110S.gb/3551378‑3551439
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
