Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,862,940 |
(A)5→6 |
coding (79/1656 nt) |
lldP ← |
L‑lactate permease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,862,935 | 1 | . | A | 100.0%
| 24.6
/ NA
| 9 | F28F (TTC→TTT) | lldP | L‑lactate permease |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (0/9); total (0/9) |
AAAGAAG‑AAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCCGGCG > W3110S.gb/3862929‑3862989
|
aaaGAAGAAAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCcggcg < 1:1424699/62‑1 (MQ=255)
aaaGAAGAAAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCcggcg < 1:1580027/62‑1 (MQ=255)
aaaGAAGAAAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCcggcg < 1:1642716/62‑1 (MQ=255)
aaaGAAGAAAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCcggcg < 1:2362793/62‑1 (MQ=255)
aaaGAAGAAAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCcggcg < 1:2516661/62‑1 (MQ=255)
aaaGAAGAAAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCcggcg < 1:2884334/62‑1 (MQ=255)
aaaGAAGAAAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCcggcg < 1:550767/62‑1 (MQ=255)
aaaGAAGAAAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCcggcg < 1:590655/62‑1 (MQ=255)
aaaGAAGAAAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCcggcg < 1:900680/62‑1 (MQ=255)
aagaagaAAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCcggcg < 1:1141031/61‑1 (MQ=255)
aagaagaAAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCcggcg < 1:141602/61‑1 (MQ=255)
|
AAAGAAG‑AAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCCGGCG > W3110S.gb/3862929‑3862989
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
