Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,260,617 |
+A |
intergenic (+20/‑90) |
dgkA → / → lexA |
diacylglycerol kinase/DNA‑binding transcriptional repressor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,260,614 | 1 | . | A | 100.0%
| 17.5
/ NA
| 7 | intergenic (+17/‑93) | dgkA/lexA | diacylglycerol kinase/DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (0/7); total (0/7) |
CCGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑AAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTT > W3110S.gb/4260554‑4260659
|
ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAt > 1:63354/1‑61 (MQ=255)
ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAt > 1:2896994/1‑61 (MQ=255)
ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a > 1:2108041/1‑62 (MQ=255)
ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a > 1:81005/1‑62 (MQ=255)
ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a > 1:366497/1‑62 (MQ=255)
ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a > 1:2728730/1‑62 (MQ=255)
ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a > 1:2628311/1‑62 (MQ=255)
ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a > 1:2575127/1‑62 (MQ=255)
ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a > 1:2218643/1‑62 (MQ=255)
ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a > 1:2109259/1‑62 (MQ=255)
ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a > 1:1623737/1‑62 (MQ=255)
ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a > 1:1528330/1‑62 (MQ=255)
ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a > 1:1463079/1‑62 (MQ=255)
ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a > 1:1337151/1‑62 (MQ=255)
ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a > 1:1241566/1‑62 (MQ=255)
ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a > 1:1066996/1‑62 (MQ=255)
ccGTGATTACCTGGTGCAATCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAt > 1:1751555/1‑61 (MQ=255)
ccTTCCAGAATTCGATAAAATCTCTTGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCttt < 1:1025761/62‑1 (MQ=255)
ccTTCCAGAATTCGATAAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCttt < 1:1018610/62‑1 (MQ=255)
ccTTCCAGAATTCGATAAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCttt < 1:1601249/62‑1 (MQ=255)
ccTTCCAGAATTCGATAAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCttt < 1:2117892/62‑1 (MQ=255)
ccTTCCAGAATTCGATAAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCttt < 1:1508733/62‑1 (MQ=255)
ccTTCCAGAATTCGATAAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCttt < 1:1106725/62‑1 (MQ=255)
ccTTCCAGAATTCGATAAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCttt < 1:780929/62‑1 (MQ=255)
|
CCGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑AAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTT > W3110S.gb/4260554‑4260659
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
