Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,583,739 |
A→G |
F94S (TTC→TCC) |
mcrB ← |
5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,583,739 | 0 | A | G | 100.0%
| 98.4
/ NA
| 30 | F94S (TTC→TCC) | mcrB | 5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrB |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/10); total (20/10) |
GAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGAACTGCCATTGGGCATGTGGTTCATTCG > W3110S.gb/4583691‑4583766
|
gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTggg > 1:1957779/1‑61 (MQ=255)
gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTggg > 1:2391446/1‑61 (MQ=255)
gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTggg > 1:2802980/1‑61 (MQ=255)
gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc > 1:974897/1‑62 (MQ=255)
gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc > 1:1068118/1‑62 (MQ=255)
gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc > 1:785035/1‑62 (MQ=255)
gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc > 1:761710/1‑62 (MQ=255)
gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc > 1:666816/1‑62 (MQ=255)
gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc > 1:663317/1‑62 (MQ=255)
gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc > 1:618895/1‑62 (MQ=255)
gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc > 1:55845/1‑62 (MQ=255)
gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc > 1:483033/1‑62 (MQ=255)
gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc > 1:297154/1‑62 (MQ=255)
gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc > 1:2901177/1‑62 (MQ=255)
gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc > 1:2473939/1‑62 (MQ=255)
gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc > 1:2370124/1‑62 (MQ=255)
gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc > 1:2224956/1‑62 (MQ=255)
gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc > 1:2165872/1‑62 (MQ=255)
gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc > 1:203838/1‑62 (MQ=255)
gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc > 1:174301/1‑62 (MQ=255)
aTACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGCATGTGGTTCATTCg < 1:2696615/62‑1 (MQ=255)
aTACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGCATGTGGTTCATTCg < 1:2913344/62‑1 (MQ=255)
aTACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGCATGTGGTTCATTCg < 1:2319776/62‑1 (MQ=255)
aTACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGCATGTGGTTCATTCg < 1:1671930/62‑1 (MQ=255)
tACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGCATGTGGTTCATTCg < 1:2537558/61‑1 (MQ=255)
tACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGCATGTGGTTCATTCg < 1:2817502/61‑1 (MQ=255)
tACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGCATGTGGTTCATTCg < 1:2197808/61‑1 (MQ=255)
tACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGCATGTGGTTCATTCg < 1:956923/61‑1 (MQ=255)
tACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGCATGTGGTTCATTCg < 1:1163348/61‑1 (MQ=255)
gTCTGAAGAGGACTGCCATTGGGCATGTGGTTCATTCg < 1:2835546/38‑1 (MQ=255)
|
GAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGAACTGCCATTGGGCATGTGGTTCATTCG > W3110S.gb/4583691‑4583766
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
