Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I2 R1	52	300.6	3297834	97.8%	3225281	57.7

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	710,816	G→A	A200V (GCG→GTG)	solA ←	N‑methyltryptophan oxidase, FAD‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	710,816	0	G	A	100.0%	67.2 / NA	22	A200V (GCG→GTG)	solA	N‑methyltryptophan oxidase, FAD‑binding
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (11/11); total (11/11)

CGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATA  >  minE/710769‑710866

cGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCACGCAGACAATCGCt                                      >  1:1031751/1‑62 (MQ=255)

cGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCACGCAGACAATCGCt                                      >  1:13265/1‑62 (MQ=255)

cGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCACGCAGACAATCGCt                                      >  1:1586428/1‑62 (MQ=255)

         cAGGCAGCTCCGGGAGCAGGTCTTTTATCCATGTTCCCACGCAGACAATCGCTTTTTTCg                               <  1:934713/60‑1 (MQ=255)

         cAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTACCCACGCAGACAATCGCTTTTTTCg                               <  1:1009138/60‑1 (MQ=255)

                             tCTTTTACCCATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGt         <  1:1012674/62‑1 (MQ=255)

                             tCTTTTACCCATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGt         <  1:979760/62‑1 (MQ=255)

                             tCTTTTACCCATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGt         <  1:560126/62‑1 (MQ=255)

                             tCTTTTACCCATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGt         <  1:50449/62‑1 (MQ=255)

                             tCTTTTACCCATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGt         <  1:445384/62‑1 (MQ=255)

                             tCTTTTACCCATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGt         <  1:360079/62‑1 (MQ=255)

                             tCTTTTACCCATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGt         <  1:3097078/62‑1 (MQ=255)

                             tCTTTTACCCATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGt         <  1:1794359/62‑1 (MQ=255)

                             tCTTTTACCCATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGt         <  1:1118488/62‑1 (MQ=255)

                                     ccATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATa  >  1:3185149/1‑61 (MQ=255)

                                     ccATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATa  >  1:3215204/1‑61 (MQ=255)

                                     ccATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATa  >  1:2867151/1‑61 (MQ=255)

                                     ccATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATa  >  1:2072631/1‑61 (MQ=255)

                                     ccATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATa  >  1:1186542/1‑61 (MQ=255)

                                     ccATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATa  >  1:784778/1‑61 (MQ=255)

                                     ccATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATa  >  1:1140530/1‑61 (MQ=255)

                                     ccATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATa  >  1:99001/1‑61 (MQ=255)

CGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATA  >  minE/710769‑710866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A