Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I2 R1
|
52 |
300.6 |
3297834 |
97.8% |
3225281 |
57.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,520,486 |
+C |
coding (403/813 nt) |
truA ← |
pseudouridylate synthase I |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,520,482 | 1 | . | C | 100.0%
| 72.2
/ NA
| 20 | V136G (GTA→GGA) | truA | pseudouridylate synthase I |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (0/20); total (0/20) |
TGCATCCGTTCAGCGTCCAGCGGTTCGTAAAAATGGGTTA‑CCCCTTTACTCAGTACCGCCG > minE/1520443‑1520503
|
tGCATCCGTTCAGCGTCCAGCGGTTCGTAAAAATGGGTTACCCCCTTTACTCAGTAccgccg < 1:248548/62‑1 (MQ=255)
tGCATCCGTTCAGCGTCCAGCGGTTCGTAAAAATGGGTTACCCCCTTTACTCAGTAccgccg < 1:941783/62‑1 (MQ=255)
tGCATCCGTTCAGCGTCCAGCGGTTCGTAAAAATGGGTTACCCCCTTTACTCAGTAccgccg < 1:911243/62‑1 (MQ=255)
tGCATCCGTTCAGCGTCCAGCGGTTCGTAAAAATGGGTTACCCCCTTTACTCAGTAccgccg < 1:907453/62‑1 (MQ=255)
tGCATCCGTTCAGCGTCCAGCGGTTCGTAAAAATGGGTTACCCCCTTTACTCAGTAccgccg < 1:879742/62‑1 (MQ=255)
tGCATCCGTTCAGCGTCCAGCGGTTCGTAAAAATGGGTTACCCCCTTTACTCAGTAccgccg < 1:831976/62‑1 (MQ=255)
tGCATCCGTTCAGCGTCCAGCGGTTCGTAAAAATGGGTTACCCCCTTTACTCAGTAccgccg < 1:781151/62‑1 (MQ=255)
tGCATCCGTTCAGCGTCCAGCGGTTCGTAAAAATGGGTTACCCCCTTTACTCAGTAccgccg < 1:756052/62‑1 (MQ=255)
tGCATCCGTTCAGCGTCCAGCGGTTCGTAAAAATGGGTTACCCCCTTTACTCAGTAccgccg < 1:385804/62‑1 (MQ=255)
tGCATCCGTTCAGCGTCCAGCGGTTCGTAAAAATGGGTTACCCCCTTTACTCAGTAccgccg < 1:2737826/62‑1 (MQ=255)
tGCATCCGTTCAGCGTCCAGCGGTTCGTAAAAATGGGTTACCCCCTTTACTCAGTAccgccg < 1:1040913/62‑1 (MQ=255)
tGCATCCGTTCAGCGTCCAGCGGTTCGTAAAAATGGGTTACCCCCTTTACTCAGTAccgccg < 1:2183687/62‑1 (MQ=255)
tGCATCCGTTCAGCGTCCAGCGGTTCGTAAAAATGGGTTACCCCCTTTACTCAGTAccgccg < 1:2150289/62‑1 (MQ=255)
tGCATCCGTTCAGCGTCCAGCGGTTCGTAAAAATGGGTTACCCCCTTTACTCAGTAccgccg < 1:1835900/62‑1 (MQ=255)
tGCATCCGTTCAGCGTCCAGCGGTTCGTAAAAATGGGTTACCCCCTTTACTCAGTAccgccg < 1:1799668/62‑1 (MQ=255)
tGCATCCGTTCAGCGTCCAGCGGTTCGTAAAAATGGGTTACCCCCTTTACTCAGTAccgccg < 1:1624132/62‑1 (MQ=255)
tGCATCCGTTCAGCGTCCAGCGGTTCGTAAAAATGGGTTACCCCCTTTACTCAGTAccgccg < 1:157995/62‑1 (MQ=255)
tGCATCCGTTCAGCGTCCAGCGGTTCGTAAAAATGGGTTACCCCCTTTACTCAGTAccgccg < 1:1255898/62‑1 (MQ=255)
tGCATCCGTTCAGCGTCCAGCGGTTCGTAAAAATGGGTTACCCCCTTTACTCAGTAccgccg < 1:1141306/62‑1 (MQ=255)
gCATCCGTTCAGCGTCCAGCGGTTCGTAAAAATGGGTTACCCCCTTTACTCAGTAccgccg < 1:2404912/61‑1 (MQ=255)
|
TGCATCCGTTCAGCGTCCAGCGGTTCGTAAAAATGGGTTA‑CCCCTTTACTCAGTACCGCCG > minE/1520443‑1520503
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
