Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I3 R1
|
26 |
437.0 |
1038814 |
65.5% |
680423 |
57.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,236,063 |
G→A |
V403V (GTG→GTA) |
purH → |
fused IMP cyclohydrolase and phosphoribosylaminoimidazolecarboxamide formyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,236,063 | 0 | G | A | 100.0%
| 25.7
/ NA
| 9 | V403V (GTG→GTA) | purH | fused IMP cyclohydrolase and phosphoribosylaminoimidazolecarboxamide formyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (0/9); total (0/9) |
CGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGAT > minE/2236042‑2236102
|
cGGTGCGGAAGAACTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGAt < 1:1005514/61‑1 (MQ=255)
cGGTGCGGAAGAACTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGAt < 1:1022810/61‑1 (MQ=255)
cGGTGCGGAAGAACTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGAt < 1:104739/61‑1 (MQ=255)
cGGTGCGGAAGAACTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGAt < 1:396966/61‑1 (MQ=255)
cGGTGCGGAAGAACTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGAt < 1:502255/61‑1 (MQ=255)
cGGTGCGGAAGAACTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGAt < 1:512654/61‑1 (MQ=255)
cGGTGCGGAAGAACTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGAt < 1:597139/61‑1 (MQ=255)
cGGTGCGGAAGAACTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGGGAt < 1:898561/61‑1 (MQ=255)
tGCGGAAGAACTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGAt < 1:971902/58‑1 (MQ=255)
|
CGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGAT > minE/2236042‑2236102
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
