Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,236,063 |
G→A |
7.3% |
V403V (GTG→GTA) |
purH → |
fused IMP cyclohydrolase and phosphoribosylaminoimidazolecarboxamide formyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,236,063 | 0 | G | A | 7.3%
| 246.3
/ 7.3
| 82 | V403V (GTG→GTA) | purH | fused IMP cyclohydrolase and phosphoribosylaminoimidazolecarboxamide formyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (43/33); new base A (4/2); total (47/35) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 |
ACGCGTGAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCGAAGTTT > minE/2235994‑2236132
|
aCGCGTGAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCgtgg > 1:3357476/1‑71 (MQ=255)
cgcgTGAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCgtggt > 1:50957/1‑71 (MQ=255)
cgcgTGAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCgtggt < 1:741185/71‑1 (MQ=255)
gcgTGAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCgtggtg < 1:3752138/71‑1 (MQ=255)
gTGAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGAc > 1:2821905/1‑71 (MQ=255)
gTGAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGAc > 1:87156/1‑71 (MQ=255)
gTGAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGAc > 1:550322/1‑71 (MQ=255)
gAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGAcc > 1:2887376/1‑70 (MQ=255)
gAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGAcc > 1:216843/1‑70 (MQ=255)
gAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGAcc > 1:212180/1‑70 (MQ=255)
aaCGGCGGTCTGCTGGTTCAGGATCGTGACCTTGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCaa < 1:3385548/71‑1 (MQ=255)
aaCGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCaa < 1:2693917/71‑1 (MQ=255)
aaCGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCa < 1:2040977/70‑1 (MQ=255)
aaCGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCa < 1:2967828/70‑1 (MQ=255)
cggcggTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAAc > 1:1105850/1‑71 (MQ=255)
cggcggTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTAGTGACCaaa > 1:522959/1‑70 (MQ=255)
gcggTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGt < 1:3872289/71‑1 (MQ=255)
cggTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCgtgg < 1:1269835/59‑1 (MQ=255)
ggTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCa < 1:2504313/64‑1 (MQ=255)
ggTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCa < 1:1228681/64‑1 (MQ=255)
gTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAg > 1:1945837/1‑71 (MQ=255)
tCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGc > 1:1193593/1‑71 (MQ=255)
ctgctgGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCgtggt > 1:1858228/1‑56 (MQ=255)
tgctgGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGcc > 1:3549680/1‑70 (MQ=255)
tgctgGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGcc > 1:3127356/1‑70 (MQ=255)
ctgGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGa < 1:3789495/55‑1 (MQ=255)
ctgGTTCAGGATCGTGACCTGGGGATGGTCGGTGCAGAAGAACTGCGCGTGGTGa < 1:3448039/55‑1 (MQ=255)
gTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCg < 1:1937503/70‑1 (MQ=255)
gTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCg > 1:897621/1‑70 (MQ=255)
cAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCgtggt > 1:1528728/1‑47 (MQ=255)
cAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGaa > 1:1789760/1‑69 (MQ=255)
cAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGaa > 1:3092543/1‑69 (MQ=255)
cAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGaa > 1:1127567/1‑69 (MQ=255)
aGGATCGTGACCTGTGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAg > 1:1949934/1‑71 (MQ=255)
aGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAAGGTCAGCCGAGCGAAc < 1:2723678/69‑1 (MQ=255)
aGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAg < 1:315723/71‑1 (MQ=255)
ggATCGTGACCTGGGTATGGTCGGTGCGGAAGAACTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAgg < 1:1232419/71‑1 (MQ=255)
ggATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAg < 1:2146291/58‑1 (MQ=255)
gATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAAc > 1:1522764/1‑52 (MQ=255)
gATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAgg > 1:2621728/1‑70 (MQ=255)
aTCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCaaa > 1:3344144/1‑50 (MQ=255)
aTCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCaaa > 1:3691751/1‑50 (MQ=255)
aTCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCaaa > 1:2794586/1‑50 (MQ=255)
tCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAAc > 1:991487/1‑71 (MQ=255)
tGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACt < 1:379868/69‑1 (MQ=255)
tGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGc > 1:3408754/1‑71 (MQ=255)
gACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGc > 1:145741/1‑70 (MQ=255)
aCCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGc > 1:1606149/1‑69 (MQ=255)
aCCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCg < 1:218925/70‑1 (MQ=255)
aCCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGt > 1:207896/1‑71 (MQ=255)
aCCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGt < 1:1881565/71‑1 (MQ=255)
cTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCa > 1:3455739/1‑40 (MQ=255)
cTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTc > 1:2001788/1‑46 (MQ=255)
gggATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATgc > 1:925057/1‑71 (MQ=255)
ggATGGTCGGTGCGGAAGAACTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATgcg > 1:1587681/1‑71 (MQ=255)
gATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATgcgc > 1:1466455/1‑71 (MQ=255)
gATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATgcgc > 1:3738092/1‑71 (MQ=255)
gTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGtt < 1:3675226/71‑1 (MQ=255)
gTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGt < 1:3248666/70‑1 (MQ=255)
tCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGaa < 1:1991946/47‑1 (MQ=255)
tCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTg > 1:2431803/1‑68 (MQ=255)
tCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTc < 1:1861229/71‑1 (MQ=255)
cGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCg > 1:1653622/1‑57 (MQ=255)
cGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTct > 1:1476088/1‑71 (MQ=255)
ggTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTct < 1:1942758/70‑1 (MQ=255)
gCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGaa > 1:2816024/1‑42 (MQ=255)
gCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTctgctg > 1:1508152/1‑71 (MQ=255)
gaagaaCTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGa < 1:2069400/70‑1 (MQ=255)
aagaaCTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAg > 1:2969952/1‑71 (MQ=255)
aagaaCTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAg > 1:114068/1‑71 (MQ=255)
aagaaCTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAg < 1:1734000/71‑1 (MQ=255)
gaaCTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTTGAAGGt < 1:2445000/71‑1 (MQ=255)
gaaCTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGt > 1:476551/1‑71 (MQ=255)
aCTGCGCGTGGTGACCAAACGTCAGCCTAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTgg < 1:3715445/71‑1 (MQ=255)
aCTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTgg > 1:708272/1‑71 (MQ=255)
aCTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTgg > 1:635947/1‑71 (MQ=255)
aCTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTgg < 1:1662669/71‑1 (MQ=255)
aCTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTgg > 1:87885/1‑71 (MQ=255)
aCTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTgg > 1:2461984/1‑71 (MQ=255)
cTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGc < 1:689216/71‑1 (MQ=255)
cTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGc < 1:2347472/71‑1 (MQ=255)
tGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGc > 1:1660803/1‑70 (MQ=255)
cgcgTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCg < 1:3828809/69‑1 (MQ=255)
cgcgTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCg > 1:711674/1‑69 (MQ=255)
cgcgTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCGa < 1:830089/70‑1 (MQ=255)
cgcgTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCg < 1:2542261/69‑1 (MQ=255)
cgTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCGAAGt < 1:2412103/71‑1 (MQ=255)
cgTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCGAAGt < 1:1413554/71‑1 (MQ=255)
cgTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCGAAGt < 1:3531321/71‑1 (MQ=255)
gtggtgACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCGAAGtt < 1:90884/71‑1 (MQ=255)
tggtgACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCGAAGttt < 1:2384842/71‑1 (MQ=255)
tggtgACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCGAAGttt < 1:1799848/71‑1 (MQ=255)
|
ACGCGTGAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCGAAGTTT > minE/2235994‑2236132
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
