Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I3 R1
|
26 |
437.0 |
1038814 |
65.5% |
680423 |
57.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,906,397 |
C→A |
G390C (GGC→TGC) |
treB ← |
fused trehalose(maltose)‑specific enzyme IIBC component of PTS |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,906,397 | 0 | C | A | 100.0%
| 189.7
/ NA
| 55 | G390C (GGC→TGC) | treB | fused trehalose(maltose)‑specific enzyme IIBC component of PTS |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (0/55); total (0/55) |
CAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAG > minE/2906346‑2906408
|
cagaACCAATCATCGCGCACAGCATC‑GGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:961207/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:76900/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:510334/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:1003857/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:539421/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:572118/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:603935/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:610854/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:614267/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:61842/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:624091/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:646654/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:715797/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:742244/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:529743/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:776481/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:780738/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:790730/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:838507/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:876379/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:887112/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:925195/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:964937/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:983177/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:991010/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:268387/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:1009876/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:114206/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:136789/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:145184/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:155472/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:156388/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:162263/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:163068/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:186966/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:309207/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:320922/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:453335/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:449341/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:447474/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:44330/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:439713/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:356942/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:341936/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:337918/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGAAGTACATTGCAg < 1:184894/62‑1 (MQ=255)
agaACCAATCATCGCGCACAGCATCGGGAAGCGAAATTTCAGGTTGATGCAGTACATTGCAg < 1:975272/62‑1 (MQ=255)
gaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:869882/61‑1 (MQ=255)
gaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:435659/61‑1 (MQ=255)
gaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:940297/61‑1 (MQ=255)
gaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:468269/61‑1 (MQ=255)
gaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:28822/61‑1 (MQ=255)
gaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:721514/61‑1 (MQ=255)
gaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:663215/61‑1 (MQ=255)
gcgcACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg < 1:192279/49‑1 (MQ=255)
|
CAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAG > minE/2906346‑2906408
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
