Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
345,099 |
C→T |
A155T (GCC→ACC) |
yahN ← |
neutral amino‑acid efflux system |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 345,099 | 0 | C | T | 100.0%
| 155.9
/ NA
| 47 | A155T (GCC→ACC) | yahN | neutral amino‑acid efflux system |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (31/16); total (31/16) |
AATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTA > W3110S.gb/345040‑345151
|
aaTTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTAt < 1:2908439/62‑1 (MQ=255)
aTTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTAtt < 1:4082574/62‑1 (MQ=255)
aTTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTAtt < 1:256399/62‑1 (MQ=255)
cacaATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt < 1:422023/61‑1 (MQ=255)
caATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa < 1:403753/52‑1 (MQ=255)
caATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa < 1:3201538/52‑1 (MQ=255)
caATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa < 1:4190591/52‑1 (MQ=255)
caATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa < 1:24915/52‑1 (MQ=255)
caATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa < 1:4070409/52‑1 (MQ=255)
caATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa < 1:3583806/52‑1 (MQ=255)
caATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa < 1:3703460/52‑1 (MQ=255)
caATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa < 1:3704351/52‑1 (MQ=255)
caATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa < 1:3782940/52‑1 (MQ=255)
caATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa < 1:823937/52‑1 (MQ=255)
aaTCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa < 1:3065757/51‑1 (MQ=255)
cccGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAAt > 1:1611622/1‑62 (MQ=255)
cccGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAAt > 1:3906346/1‑62 (MQ=255)
cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt > 1:3584998/1‑52 (MQ=255)
cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt > 1:3308236/1‑52 (MQ=255)
cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt > 1:3255419/1‑52 (MQ=255)
cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt > 1:3234027/1‑52 (MQ=255)
cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt > 1:3213816/1‑52 (MQ=255)
cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt > 1:1027497/1‑52 (MQ=255)
cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt > 1:2979078/1‑52 (MQ=255)
cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt > 1:2726047/1‑52 (MQ=255)
cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt > 1:57338/1‑52 (MQ=255)
cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt > 1:604478/1‑52 (MQ=255)
cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt > 1:2381079/1‑52 (MQ=255)
cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt > 1:2269043/1‑52 (MQ=255)
cAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGa > 1:1573600/1‑61 (MQ=255)
cccATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGtt > 1:2408057/1‑62 (MQ=255)
cccATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGtt > 1:1786855/1‑62 (MQ=255)
cccATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGtt > 1:753501/1‑62 (MQ=255)
gTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACgg > 1:4060164/1‑55 (MQ=255)
gTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCgg > 1:752549/1‑62 (MQ=255)
gTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCgg > 1:930859/1‑62 (MQ=255)
gTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCgg > 1:347276/1‑62 (MQ=255)
gTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCgg > 1:2976525/1‑62 (MQ=255)
gTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCgg > 1:2519864/1‑62 (MQ=255)
gTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCgg > 1:2410649/1‑62 (MQ=255)
gTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCgg > 1:2090255/1‑62 (MQ=255)
gTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCgg > 1:1566764/1‑62 (MQ=255)
gTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCgg > 1:1293199/1‑62 (MQ=255)
ggTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGt < 1:1646036/61‑1 (MQ=255)
ttCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTa > 1:311526/1‑58 (MQ=255)
ttCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTa > 1:4124965/1‑58 (MQ=255)
ttCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTa > 1:2766904/1‑58 (MQ=255)
|
AATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTA > W3110S.gb/345040‑345151
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
