Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
527,659 |
A→G |
K163E (AAA→GAA) |
ylbH → |
conserved hypothetical protein, rhs‑like |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 527,659 | 0 | A | G | 100.0%
| 27.8
/ NA
| 9 | K163E (AAA→GAA) | ylbH | conserved hypothetical protein, rhs‑like |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (0/9); total (0/9) |
GTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGAAAA > W3110S.gb/527626‑527709
|
gtTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATc < 1:3985410/61‑1 (MQ=255)
gtTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCt < 1:1037032/62‑1 (MQ=255)
gtTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCt < 1:3222579/62‑1 (MQ=255)
gtTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCt < 1:539594/62‑1 (MQ=255)
cGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATc < 1:1310220/51‑1 (MQ=255)
cGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATc < 1:1355732/51‑1 (MQ=255)
cGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATc < 1:3336192/51‑1 (MQ=255)
tGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGaaaa < 1:222711/62‑1 (MQ=255)
atATGAAGAAGAGATTAGAGATTTCGGGTTAAATc < 1:2952999/34‑1 (MQ=25)
|
GTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGAAAA > W3110S.gb/527626‑527709
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
