Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
639,164 |
G→A |
P63P (CCG→CCA) |
ahpF → |
alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 639,164 | 0 | G | A | 100.0%
| 166.8
/ NA
| 52 | P63P (CCG→CCA) | ahpF | alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (45/7); total (45/7) |
GCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGT > W3110S.gb/639105‑639206
|
gCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATc < 1:2717391/62‑1 (MQ=255)
gCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATc < 1:1343321/62‑1 (MQ=255)
gCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATc < 1:3981175/62‑1 (MQ=255)
gCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATc < 1:4012784/62‑1 (MQ=255)
aGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATCtt < 1:3719368/62‑1 (MQ=255)
aCTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATCTTTc > 1:2002758/1‑61 (MQ=255)
gTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTGa < 1:1854766/62‑1 (MQ=255)
tAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATc < 1:605431/36‑1 (MQ=255)
aagaagATAACAGCTTGCCGGTGCGTAAGCCATCt > 1:1832169/1‑35 (MQ=255)
aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTg > 1:330983/1‑41 (MQ=255)
aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTg > 1:3813814/1‑41 (MQ=255)
aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTg > 1:4121495/1‑41 (MQ=255)
aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTg > 1:4201119/1‑41 (MQ=255)
aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTg > 1:1619527/1‑41 (MQ=255)
aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTg > 1:550810/1‑41 (MQ=255)
aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCAGATcgg > 1:4124989/1‑44 (MQ=38)
aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCAGATcgg > 1:4113302/1‑44 (MQ=38)
aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCAGATcgg > 1:428485/1‑44 (MQ=38)
aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCAGATcgg > 1:450985/1‑44 (MQ=38)
aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCAGATcgg > 1:375286/1‑44 (MQ=38)
aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCAGATcgg > 1:3746373/1‑44 (MQ=38)
aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCAGATcgg > 1:3601471/1‑44 (MQ=38)
aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCAGATcgg > 1:1167804/1‑44 (MQ=38)
aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCAGATcgg > 1:702338/1‑44 (MQ=38)
aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCAGATcgg > 1:2759817/1‑44 (MQ=38)
aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCAAGATcg > 1:2126314/1‑44 (MQ=37)
gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCa > 1:1220764/1‑48 (MQ=255)
gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCa > 1:1233591/1‑48 (MQ=255)
gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCa > 1:4269178/1‑48 (MQ=255)
gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCa > 1:2074665/1‑48 (MQ=255)
gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCa > 1:2241341/1‑48 (MQ=255)
gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCa > 1:3978238/1‑48 (MQ=255)
gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCa > 1:3100105/1‑48 (MQ=255)
gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCa > 1:2285835/1‑48 (MQ=255)
gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCa > 1:2399774/1‑48 (MQ=255)
gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACg > 1:219852/1‑61 (MQ=255)
gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGt > 1:273176/1‑62 (MQ=255)
gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGt > 1:2555469/1‑62 (MQ=255)
gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGt > 1:3764696/1‑62 (MQ=255)
gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGt > 1:1691977/1‑62 (MQ=255)
gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGt > 1:1768027/1‑62 (MQ=255)
gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGt > 1:4457451/1‑62 (MQ=255)
ggTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc > 1:3464039/1‑44 (MQ=255)
ggTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc > 1:1331299/1‑44 (MQ=255)
ggTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc > 1:4597964/1‑44 (MQ=255)
ggTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc > 1:4568443/1‑44 (MQ=255)
ggTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc > 1:4276531/1‑44 (MQ=255)
ggTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc > 1:1937280/1‑44 (MQ=255)
ggTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc > 1:195727/1‑44 (MQ=255)
ggTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc > 1:2714060/1‑44 (MQ=255)
ggTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc > 1:3984092/1‑44 (MQ=255)
ggTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc > 1:227015/1‑44 (MQ=255)
|
GCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGT > W3110S.gb/639105‑639206
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
