Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
976,718 |
G→A |
E216E (GAG→GAA) |
mukE → |
protein involved in chromosome partitioning |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 976,718 | 0 | G | A | 100.0%
| 136.3
/ NA
| 42 | E216E (GAG→GAA) | mukE | protein involved in chromosome partitioning |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/29); total (13/29) |
CCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCT > W3110S.gb/976677‑976772
|
ccGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGa < 1:852210/53‑1 (MQ=255)
ccGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGa < 1:1336510/53‑1 (MQ=255)
ccGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGa < 1:297735/53‑1 (MQ=255)
ccGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGa < 1:238524/53‑1 (MQ=255)
ccGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGga < 1:3652930/60‑1 (MQ=255)
ccGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGga < 1:725048/60‑1 (MQ=255)
ccGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGga < 1:2858036/60‑1 (MQ=255)
ccGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGga < 1:809336/60‑1 (MQ=255)
ccGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGga < 1:2735645/60‑1 (MQ=255)
ccGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGga < 1:2617644/60‑1 (MQ=255)
ccGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGga < 1:4978/60‑1 (MQ=255)
ccGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGga < 1:1234477/60‑1 (MQ=255)
cGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGgaga > 1:1809763/1‑61 (MQ=255)
cGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGgaga > 1:1392960/1‑61 (MQ=255)
cGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGgaga > 1:1011751/1‑61 (MQ=255)
ttGAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGGAGAGgaa < 1:3891940/62‑1 (MQ=255)
ttGAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGGAGAGgaa < 1:3784204/62‑1 (MQ=255)
gAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGGAGAGgaag > 1:2247812/1‑61 (MQ=255)
gAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGGAGAGgaag > 1:257995/1‑61 (MQ=255)
gAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGGAGAGgaag > 1:4524376/1‑61 (MQ=255)
gAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGGAGAGgaag > 1:1423608/1‑61 (MQ=255)
gAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGGAGAGgaag > 1:3712043/1‑61 (MQ=255)
aaaaTCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGGAGAGgaaga > 1:3818965/1‑61 (MQ=255)
catcTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATg > 1:2880387/1‑62 (MQ=255)
cAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGaa > 1:521199/1‑62 (MQ=255)
cAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGaa > 1:3422100/1‑62 (MQ=255)
cAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGa > 1:3109812/1‑61 (MQ=255)
aTGAAACCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAAt < 1:3558539/62‑1 (MQ=255)
aTGAAACCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAAt < 1:3446813/62‑1 (MQ=255)
aTGAAACCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAAt < 1:4309082/62‑1 (MQ=255)
aTGAAACCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAAt < 1:1917392/62‑1 (MQ=255)
aaaCCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTc < 1:3009477/62‑1 (MQ=255)
aaaCCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTc < 1:526027/62‑1 (MQ=255)
aaaCCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTc < 1:4329175/62‑1 (MQ=255)
aaaCCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTc < 1:1674783/62‑1 (MQ=255)
aCCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTaaa < 1:723932/56‑1 (MQ=255)
aCCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTaaa < 1:3337075/56‑1 (MQ=255)
aCCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTaaa < 1:421352/56‑1 (MQ=255)
aCCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTaaa < 1:3742892/56‑1 (MQ=255)
aCCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTaaa < 1:3223494/56‑1 (MQ=255)
aCCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTaaa < 1:2758147/56‑1 (MQ=255)
ccGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCt < 1:1209483/62‑1 (MQ=255)
|
CCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCT > W3110S.gb/976677‑976772
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
