Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,007,501 |
A→C |
N174K (AAT→AAG) |
ycbX ← |
predicted 2Fe‑2S cluster‑containing protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,007,501 | 0 | A | C | 100.0%
| 214.5
/ NA
| 57 | N174K (AAT→AAG) | ycbX | predicted 2Fe‑2S cluster‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (42/15); total (42/15) |
CAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGATTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGTTGTTGGAG > W3110S.gb/1007443‑1007551
|
cAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTg < 1:2970770/62‑1 (MQ=255)
cAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTg < 1:4168029/62‑1 (MQ=255)
cAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTg < 1:2286204/62‑1 (MQ=255)
tGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGCCGCGCCGGAAACCACCAGCTTgggg > 1:649506/1‑62 (MQ=255)
tGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTgggg > 1:1128917/1‑62 (MQ=255)
tGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTgggg > 1:1349740/1‑62 (MQ=255)
tGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTgggg > 1:612351/1‑62 (MQ=255)
tGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTgggg > 1:2284531/1‑62 (MQ=255)
cGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCtt > 1:1548138/1‑56 (MQ=255)
cGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGc > 1:4654860/1‑61 (MQ=255)
cGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGc > 1:4089518/1‑61 (MQ=255)
cGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGc > 1:54247/1‑61 (MQ=255)
cACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAAtt > 1:2976915/1‑62 (MQ=255)
cTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGc > 1:164998/1‑62 (MQ=255)
cTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGc > 1:1187273/1‑62 (MQ=255)
cTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGc > 1:2823108/1‑62 (MQ=255)
cTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGc > 1:2693470/1‑62 (MQ=255)
cTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGc > 1:2663425/1‑62 (MQ=255)
cTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGc > 1:635642/1‑62 (MQ=255)
cTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGc > 1:2558737/1‑62 (MQ=255)
cTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGc > 1:24627/1‑62 (MQ=255)
cTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGc > 1:1648458/1‑62 (MQ=255)
cTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGc > 1:543615/1‑62 (MQ=255)
tttCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCt < 1:412404/62‑1 (MQ=255)
tttCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCt < 1:1453175/62‑1 (MQ=255)
tttCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCt < 1:4529978/62‑1 (MQ=255)
tttCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCt < 1:1597931/62‑1 (MQ=255)
tttCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCt < 1:344323/62‑1 (MQ=255)
ttCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCt < 1:3450828/61‑1 (MQ=255)
ttCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCt < 1:3445242/61‑1 (MQ=255)
cAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTcc < 1:4113409/60‑1 (MQ=255)
cAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTcc < 1:970182/60‑1 (MQ=255)
cAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCa < 1:3438152/61‑1 (MQ=255)
cAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCa < 1:877994/61‑1 (MQ=255)
cAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAt > 1:2803324/1‑62 (MQ=255)
cAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAt > 1:1049242/1‑62 (MQ=255)
aGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCa < 1:3122343/60‑1 (MQ=255)
ccAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCg > 1:3815709/1‑62 (MQ=255)
ccAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCg > 1:2530233/1‑62 (MQ=255)
ccAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCg > 1:2476553/1‑62 (MQ=255)
ccAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCg > 1:2388185/1‑62 (MQ=255)
cgcgCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAtttt > 1:3481359/1‑42 (MQ=255)
cgcgCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAtttt > 1:3708982/1‑42 (MQ=255)
cgcgCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAtttt > 1:945758/1‑42 (MQ=255)
cgcgCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAtttt > 1:1095966/1‑42 (MQ=255)
cgcgCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAtttt > 1:1404854/1‑42 (MQ=255)
cgcgCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAtttt > 1:2059053/1‑42 (MQ=255)
cgcgCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAtttt > 1:236528/1‑42 (MQ=255)
cgcgCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAtttt > 1:4630305/1‑42 (MQ=255)
cgcgCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAtttt > 1:4257729/1‑42 (MQ=255)
cgcgCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAtttt > 1:2646487/1‑42 (MQ=255)
aaaCCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGTTGTTGgag > 1:4545534/1‑61 (MQ=255)
aaaCCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGTTGTTGgag > 1:1978547/1‑61 (MQ=255)
aaaCCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGTTGTTGgag > 1:1571188/1‑61 (MQ=255)
aaaCCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGTTGTTGgag > 1:4397146/1‑61 (MQ=255)
aaaCCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGTTGTTGgag > 1:3496105/1‑61 (MQ=255)
aaaCCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGTTGTTGgag > 1:2467151/1‑61 (MQ=255)
|
CAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGATTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGTTGTTGGAG > W3110S.gb/1007443‑1007551
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
