Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,084,947 |
A→G |
K384E (AAA→GAA) |
ycdB → |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,084,947 | 0 | A | G | 100.0%
| 78.5
/ NA
| 22 | K384E (AAA→GAA) | ycdB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (4/18); total (4/18) |
GTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTT > W3110S.gb/1084902‑1085001
|
gTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGa > 1:4548798/1‑62 (MQ=255)
aaCACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTgga > 1:1446359/1‑61 (MQ=255)
aaCACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTgga > 1:1557847/1‑61 (MQ=255)
aaCACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTgga > 1:2008522/1‑61 (MQ=255)
aaaaGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAAc < 1:1210521/62‑1 (MQ=255)
aaaaGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAAc < 1:3272552/62‑1 (MQ=255)
aaaaGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAAc < 1:1127358/62‑1 (MQ=255)
aaaaGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAAc < 1:1859636/62‑1 (MQ=255)
aaaaGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAAc < 1:2827535/62‑1 (MQ=255)
aaaGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAAc < 1:465951/61‑1 (MQ=255)
tGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATcggcg < 1:4344981/60‑1 (MQ=255)
tGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATcggcg < 1:3102668/60‑1 (MQ=255)
tGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATcggcg < 1:306819/60‑1 (MQ=255)
tGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATcggcg < 1:2340766/60‑1 (MQ=255)
tGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATcggcg < 1:1470124/60‑1 (MQ=255)
tGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATcggcg < 1:1279829/60‑1 (MQ=255)
aGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGtt < 1:468920/62‑1 (MQ=255)
aGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGtt < 1:840947/62‑1 (MQ=255)
aGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGtt < 1:4318526/62‑1 (MQ=255)
aGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGtt < 1:2524886/62‑1 (MQ=255)
aGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGtt < 1:2008835/62‑1 (MQ=255)
aGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGtt < 1:1826826/62‑1 (MQ=255)
|
GTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTT > W3110S.gb/1084902‑1085001
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
