Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,224,919 |
G→A |
intergenic (‑423/‑123) |
ymgG ← / → ymgH |
hypothetical protein/hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,224,919 | 0 | G | A | 100.0%
| 83.8
/ NA
| 26 | intergenic (‑423/‑123) | ymgG/ymgH | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (4/22); total (4/22) |
ATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTAC > W3110S.gb/1224873‑1224962
|
aTTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGtattat < 1:913916/61‑1 (MQ=255)
aTTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGtattat < 1:853109/61‑1 (MQ=255)
aTTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGtattat < 1:1744085/61‑1 (MQ=255)
aTTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGtattat < 1:4424908/61‑1 (MQ=255)
aTTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGtattat < 1:4240871/61‑1 (MQ=255)
aTTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGtattat < 1:1973363/61‑1 (MQ=255)
aTTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGtattat < 1:3950069/61‑1 (MQ=255)
aTTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGtattat < 1:1288464/61‑1 (MQ=255)
ttAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGtattat < 1:3230000/60‑1 (MQ=255)
ttAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGcattat < 1:1741502/60‑1 (MQ=21)
cATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa < 1:3619646/62‑1 (MQ=255)
cATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa < 1:716857/62‑1 (MQ=255)
cATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa < 1:4487621/62‑1 (MQ=255)
cATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa < 1:4223623/62‑1 (MQ=255)
cATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa < 1:3885945/62‑1 (MQ=255)
cATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa < 1:2492335/62‑1 (MQ=255)
cATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa < 1:188261/62‑1 (MQ=255)
cATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa < 1:186721/62‑1 (MQ=255)
cATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa < 1:1668393/62‑1 (MQ=255)
cATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa < 1:1645533/62‑1 (MQ=255)
cATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa < 1:1624981/62‑1 (MQ=255)
attttattttGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa < 1:1728450/61‑1 (MQ=255)
ttattttGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGt > 1:741566/1‑62 (MQ=255)
gATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTac > 1:4142484/1‑51 (MQ=255)
gATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTac > 1:2045729/1‑51 (MQ=255)
gATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTac > 1:4403186/1‑51 (MQ=255)
|
ATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTAC > W3110S.gb/1224873‑1224962
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
