Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,394,475 |
(A)8→9 |
coding (771/900 nt) |
ycjZ → |
predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,394,467 | 1 | . | A | 100.0%
| 62.4
/ NA
| 20 | E255K (GAA→AAA) | ycjZ | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (7/13); total (7/13) |
ATTGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑AAAAAAAACTGATACGTGTTTTGGATAAATTCACACCAGATTTACCC > W3110S.gb/1394410‑1394514
|
aTTGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑aaaa < 1:617332/62‑1 (MQ=255)
aTTGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑aaaa < 1:4186931/62‑1 (MQ=255)
aTTGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑aaaa < 1:1684966/62‑1 (MQ=255)
aTTGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑aaaa < 1:908442/62‑1 (MQ=255)
tGATGGGCTTGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑aaaaaa < 1:2375092/62‑1 (MQ=255)
tGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑aaaaaa < 1:2926224/62‑1 (MQ=255)
tGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑aaaaaa < 1:4060460/62‑1 (MQ=255)
tGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑aaaaaa < 1:3729558/62‑1 (MQ=255)
tGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑aaaaaa < 1:1653431/62‑1 (MQ=255)
gATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑a < 1:3007117/56‑1 (MQ=255)
cGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGttt < 1:997669/61‑1 (MQ=255)
cGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGttt < 1:1481798/61‑1 (MQ=255)
cGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGttt < 1:3983037/61‑1 (MQ=255)
cGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGttt < 1:831088/61‑1 (MQ=255)
tATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTca > 1:1270935/1‑62 (MQ=255)
tATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTca > 1:2861979/1‑62 (MQ=255)
tATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTca > 1:4051197/1‑62 (MQ=255)
tATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTca > 1:1580566/1‑62 (MQ=255)
tGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTcac > 1:2692312/1‑61 (MQ=255)
tGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTcac > 1:2379823/1‑61 (MQ=255)
gATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTcac < 1:1843321/60‑1 (MQ=255)
gATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTcac < 1:4060499/60‑1 (MQ=255)
gATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTcac < 1:2047422/60‑1 (MQ=255)
gATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTcac < 1:4391848/60‑1 (MQ=255)
gATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTcac < 1:2130440/60‑1 (MQ=255)
ggTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTCACACCAGa > 1:3404761/1‑61 (MQ=255)
gAGCGGGCTATTAAAGAAAAAAAAACTGATACGTCttt < 1:3763111/38‑1 (MQ=25)
gCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTCACACCAGATTTAcc < 1:4399271/61‑1 (MQ=255)
cGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTCACACCAGATTTAccc < 1:3619547/61‑1 (MQ=255)
gggCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTCACACCAGATTTAccc < 1:1293320/60‑1 (MQ=255)
|
ATTGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑AAAAAAAACTGATACGTGTTTTGGATAAATTCACACCAGATTTACCC > W3110S.gb/1394410‑1394514
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
