Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,358,400 |
G→A |
A362T (GCT→ACT) |
glpA → |
sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,358,400 | 0 | G | A | 100.0%
| 138.0
/ NA
| 42 | A362T (GCT→ACT) | glpA | sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (7/35); total (7/35) |
GGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGAC > W3110S.gb/2358352‑2358458
|
ggATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAc < 1:1189464/62‑1 (MQ=255)
ggATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAc < 1:456297/62‑1 (MQ=255)
ggATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAc < 1:647027/62‑1 (MQ=255)
ggATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAc < 1:282834/62‑1 (MQ=255)
ggATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAc < 1:757555/62‑1 (MQ=255)
ggATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAc < 1:248292/62‑1 (MQ=255)
ggATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAc < 1:2366680/62‑1 (MQ=255)
gATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAcc < 1:1963660/62‑1 (MQ=255)
gATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAcc < 1:1326014/62‑1 (MQ=255)
gATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAcc < 1:2862870/62‑1 (MQ=255)
tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcgc < 1:831357/61‑1 (MQ=255)
tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcgc < 1:2290987/61‑1 (MQ=255)
tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcgc < 1:1648664/61‑1 (MQ=255)
tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcgc < 1:146275/61‑1 (MQ=255)
tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcgc < 1:27175/61‑1 (MQ=255)
tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcgc < 1:3546114/61‑1 (MQ=255)
tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcgc < 1:974756/61‑1 (MQ=255)
tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTAATGACTGAATGGGCTACCGAcgc < 1:2866768/61‑1 (MQ=255)
ccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAcc < 1:3978713/53‑1 (MQ=255)
accGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAcc < 1:3644510/48‑1 (MQ=255)
accGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAcc < 1:1823670/48‑1 (MQ=255)
accGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAcc < 1:4671143/48‑1 (MQ=255)
accGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAcc < 1:96743/48‑1 (MQ=255)
accGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAcc < 1:1875152/48‑1 (MQ=255)
gCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcgc < 1:990599/46‑1 (MQ=255)
aaaCTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcg < 1:1018153/43‑1 (MQ=255)
aaaCTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcg < 1:3848561/43‑1 (MQ=255)
aaaCTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcg < 1:1290296/43‑1 (MQ=255)
aaaCTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcg < 1:4117312/43‑1 (MQ=255)
aaaCTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcg < 1:1837661/43‑1 (MQ=255)
aaCTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcg < 1:3176548/42‑1 (MQ=255)
aaCTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcg < 1:2911210/42‑1 (MQ=255)
aCTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcg < 1:965191/41‑1 (MQ=255)
cTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTa < 1:3707039/45‑1 (MQ=255)
cTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTa < 1:4120563/45‑1 (MQ=255)
tgaCCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACAcg > 1:3302929/1‑62 (MQ=255)
tgaCCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACAcg > 1:532953/1‑62 (MQ=255)
tgaCCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACAcg > 1:1191361/1‑62 (MQ=255)
ggCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGt > 1:3317598/1‑62 (MQ=255)
aTGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGAc > 1:4134231/1‑62 (MQ=255)
aTGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGAc > 1:2701046/1‑62 (MQ=255)
aTGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGAc > 1:3758574/1‑62 (MQ=255)
|
GGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGAC > W3110S.gb/2358352‑2358458
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
