Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,493,033 |
C→T |
Q1072* (CAG→TAG) |
evgS → |
hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,493,033 | 0 | C | T | 100.0%
| 337.0
/ NA
| 101 | Q1072* (CAG→TAG) | evgS | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (45/56); total (45/56) |
Only 100 of 101 total aligned reads displayed.
GTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGAT > W3110S.gb/2492986‑2493080
|
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACAcc > 1:4274860/1‑57 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAg < 1:4183555/60‑1 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAg < 1:2201197/60‑1 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAg < 1:211419/60‑1 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAg < 1:629422/60‑1 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAg < 1:4666343/60‑1 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAg < 1:3268067/60‑1 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAg < 1:115035/60‑1 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAg < 1:3702139/60‑1 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAg < 1:3707367/60‑1 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt > 1:3679524/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt > 1:3503653/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt > 1:3292686/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt > 1:3269835/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt > 1:3252779/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt > 1:315281/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt > 1:3901512/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt > 1:4069829/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt > 1:2992267/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt > 1:2914383/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt > 1:555451/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt > 1:2582645/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt > 1:1427428/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt > 1:105504/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt > 1:2573494/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt > 1:1383106/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt > 1:1147925/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGt > 1:2407928/1‑61 (MQ=255)
gtatgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAg < 1:572255/57‑1 (MQ=255)
tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt < 1:4299348/48‑1 (MQ=255)
tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt < 1:1498852/48‑1 (MQ=255)
tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt < 1:3323090/48‑1 (MQ=255)
tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt < 1:3484112/48‑1 (MQ=255)
tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt < 1:3152444/48‑1 (MQ=255)
tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt < 1:1319096/48‑1 (MQ=255)
tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt < 1:604463/48‑1 (MQ=255)
tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt < 1:1087115/48‑1 (MQ=255)
tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt < 1:2813813/48‑1 (MQ=255)
tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt < 1:3827847/48‑1 (MQ=255)
tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt < 1:581654/48‑1 (MQ=255)
tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt < 1:3846399/48‑1 (MQ=255)
tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt < 1:2453619/48‑1 (MQ=255)
ttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTgcgc < 1:4455501/61‑1 (MQ=255)
ttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTgcgc < 1:4189801/61‑1 (MQ=255)
aCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCa > 1:3845615/1‑48 (MQ=255)
aCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCa > 1:4036306/1‑48 (MQ=255)
aCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCa > 1:310422/1‑48 (MQ=255)
aCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCa > 1:3595912/1‑48 (MQ=255)
aCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCa > 1:2622686/1‑48 (MQ=255)
aCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCa > 1:2623995/1‑48 (MQ=255)
aCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCa > 1:3693286/1‑48 (MQ=255)
aCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCa > 1:4488416/1‑48 (MQ=255)
ggATGTACTGAAGACACATTTAAGTTAGTTACACCAAGTTg < 1:4254050/41‑1 (MQ=255)
ggATGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTg < 1:3888047/41‑1 (MQ=255)
ggATGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTg < 1:1726831/41‑1 (MQ=255)
gATGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATc > 1:771458/1‑61 (MQ=255)
gATGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATc > 1:2963593/1‑61 (MQ=255)
gATGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATc > 1:1732361/1‑61 (MQ=255)
gATGTAATGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATc > 1:1065781/1‑61 (MQ=255)
tttACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGcc < 1:709917/60‑1 (MQ=255)
tGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGcc < 1:4459254/62‑1 (MQ=255)
tGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGcc < 1:515395/62‑1 (MQ=255)
tGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGcc < 1:48092/62‑1 (MQ=255)
tGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGcc < 1:2853131/62‑1 (MQ=255)
tGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGcc < 1:3644918/62‑1 (MQ=255)
tGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGcc < 1:3639454/62‑1 (MQ=255)
tGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGcc < 1:752550/62‑1 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat > 1:4406607/1‑41 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat > 1:4154983/1‑41 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat > 1:3604743/1‑41 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat > 1:3809163/1‑41 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat > 1:2584932/1‑41 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat > 1:3740687/1‑41 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat > 1:3718362/1‑41 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat > 1:102002/1‑41 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat > 1:3362177/1‑41 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat > 1:2778445/1‑41 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat > 1:3082409/1‑41 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat > 1:3047117/1‑41 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat > 1:190014/1‑41 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc < 1:2071254/61‑1 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc < 1:712485/61‑1 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc < 1:229491/61‑1 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc < 1:2436913/61‑1 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc < 1:2712344/61‑1 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc < 1:4876/61‑1 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc < 1:1127660/61‑1 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc < 1:3352255/61‑1 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc < 1:444526/61‑1 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc < 1:974025/61‑1 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc < 1:3754498/61‑1 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc < 1:4220757/61‑1 (MQ=255)
cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc < 1:978474/61‑1 (MQ=255)
aaCACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGat < 1:2844023/61‑1 (MQ=255)
aaCACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGat < 1:1941350/61‑1 (MQ=255)
aaCACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGat < 1:621170/61‑1 (MQ=255)
aaCACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGat < 1:4065960/61‑1 (MQ=255)
aaCACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGat < 1:4638225/61‑1 (MQ=255)
aaCACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGat < 1:4262120/61‑1 (MQ=255)
aaCACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGa < 1:4002287/60‑1 (MQ=255)
|
GTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGAT > W3110S.gb/2492986‑2493080
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
