Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,523,255 |
(T)6→7 |
coding (24/2190 nt) |
yfeA ← |
predicted diguanylate cyclase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,523,249 | 1 | . | T | 100.0%
| 133.2
/ NA
| 41 | N10K (AAT→AAA) | yfeA | predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (30/11); total (30/11) |
GGATAGCTGAATAAGTACCACGGTGAGCGTAAACGCTAGTGTGAATATCTTGATA‑TTTTTTATCAGGTTATGCTCCACAAACATGAGTAC > W3110S.gb/2523195‑2523284
|
ggATAGCTGAATAAGTACCACGGTGAGCGTAAACGCTAGTGTGAATATCTTGATA‑ttttttt < 1:2139635/62‑2 (MQ=255)
tAAGTACCACGGTGAGCGTAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATg > 1:887974/1‑62 (MQ=255)
tAAGTACCACGGTGAGCGTAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATg > 1:4438272/1‑62 (MQ=255)
aGTACCACGGTGAGCGTAAACTCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCt < 1:1327679/62‑1 (MQ=255)
aGTACCACGGTGAGCGTAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCt < 1:2945899/62‑1 (MQ=255)
aGTACCACGGTGAGCGTAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCt < 1:4086305/62‑1 (MQ=255)
aGTACCACGGTGAGCGTAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCt < 1:3826427/62‑1 (MQ=255)
aGTACCACGGTGAGCGTAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCt < 1:2919210/62‑1 (MQ=255)
aGTACCACGGTGAGCGTAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCt < 1:258510/62‑1 (MQ=255)
aGTACCACGGTGAGCGTAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCt < 1:197057/62‑1 (MQ=255)
aCCACGGTGAGCGTAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCca < 1:4375576/62‑1 (MQ=255)
aCCACGGTGAGCGTAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCca < 1:3741994/62‑1 (MQ=255)
aCCACGGTGAGCGTAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCca < 1:2021681/62‑1 (MQ=255)
aCCACGGTGAGCGTAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCca < 1:2812966/62‑1 (MQ=255)
gTGAGCGTAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACAAAc > 1:2284783/1‑61 (MQ=255)
gTGAGCGTAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACAAAc > 1:1782631/1‑61 (MQ=255)
gTGAGCGTAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACAAAc > 1:3067956/1‑61 (MQ=255)
gTGAGAGTAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACAAAc > 1:2307557/1‑61 (MQ=255)
tAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTcc > 1:4353486/1‑48 (MQ=39)
tAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACaaa > 1:931000/1‑53 (MQ=255)
tAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACaaa > 1:1430040/1‑53 (MQ=255)
tAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACaaa > 1:608744/1‑53 (MQ=255)
tAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACaaa > 1:4473439/1‑53 (MQ=255)
tAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACaaa > 1:4348020/1‑53 (MQ=255)
tAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACaaa > 1:2356333/1‑53 (MQ=255)
tAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACaaa > 1:1941273/1‑53 (MQ=255)
tAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACaaa > 1:2383947/1‑53 (MQ=255)
tAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACaaa > 1:1097722/1‑53 (MQ=255)
tAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACAAACATGAGTac > 1:3666753/1‑62 (MQ=255)
tAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACAAACATGAGTac > 1:2473793/1‑62 (MQ=255)
tAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACAAACATGAGTac > 1:2341611/1‑62 (MQ=255)
tAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACAAACATGAGTac > 1:4438187/1‑62 (MQ=255)
tAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACAAACATGAGTac > 1:2504409/1‑62 (MQ=255)
tAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACAAACATGAGTac > 1:618147/1‑62 (MQ=255)
tAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACAAACATGAGTac > 1:640817/1‑62 (MQ=255)
tAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACAAACATGAGTac > 1:693088/1‑62 (MQ=255)
tAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACAAACATGAGTac > 1:2654273/1‑62 (MQ=255)
tAAACGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACAAACATGAGTa > 1:3859444/1‑61 (MQ=255)
aCGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACAAACATGAGTa > 1:3666360/1‑58 (MQ=255)
aCGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACAAACATGAGTa > 1:148539/1‑58 (MQ=255)
aCGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTCCACAAACATGAGTa > 1:2671780/1‑58 (MQ=255)
aCGCTAGTGTGAATATCTTGATATTTTTTTATCAGGTTATGCTACACAAACATGAGTa > 1:4422304/1‑58 (MQ=255)
|
GGATAGCTGAATAAGTACCACGGTGAGCGTAAACGCTAGTGTGAATATCTTGATA‑TTTTTTATCAGGTTATGCTCCACAAACATGAGTAC > W3110S.gb/2523195‑2523284
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
